scholarly journals Crystal structure of NAD+-dependent Peptoniphilus asaccharolyticus glutamate dehydrogenase reveals determinants of cofactor specificity

2012 ◽  
Vol 177 (2) ◽  
pp. 543-552 ◽  
Author(s):  
Tânia Oliveira ◽  
Santosh Panjikar ◽  
John B. Carrigan ◽  
Muaawia Hamza ◽  
Michael A. Sharkey ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Glutamate Dehydrogenase ◽  
Cofactor Specificity

scholarly journals Crystal structure of a chimaeric bacterial glutamate dehydrogenase

2016 ◽  
Vol 72 (6) ◽  
pp. 462-466 ◽  
Author(s):  
Tânia Oliveira ◽  
Michael A. Sharkey ◽  
Paul C. Engel ◽  
Amir R. Khan
Keyword(s):  
Crystal Structure ◽  
Glutamate Dehydrogenase ◽  
Rigid Bodies ◽  
Nucleotide Binding Domain ◽  
Oxidative Deamination ◽  
E Coli ◽  
Cofactor Binding ◽  
Signature Sequences ◽  
Clostridium Symbiosum ◽  
Domain I

Glutamate dehydrogenases (EC 1.4.1.2–4) catalyse the oxidative deamination of L-glutamate to α-ketoglutarate using NAD(P)+as a cofactor. The bacterial enzymes are hexameric, arranged with 32 symmetry, and each polypeptide consists of an N-terminal substrate-binding segment (domain I) followed by a C-terminal cofactor-binding segment (domain II). The catalytic reaction takes place in the cleft formed at the junction of the two domains. Distinct signature sequences in the nucleotide-binding domain have been linked to the binding of NAD+versusNADP+, but they are not unambiguous predictors of cofactor preference. In the absence of substrate, the two domains move apart as rigid bodies, as shown by the apo structure of glutamate dehydrogenase fromClostridium symbiosum. Here, the crystal structure of a chimaeric clostridial/Escherichia colienzyme has been determined in the apo state. The enzyme is fully functional and reveals possible determinants of interdomain flexibility at a hinge region following the pivot helix. The enzyme retains the preference for NADP+cofactor from the parentE. colidomain II, although there are subtle differences in catalytic activity.

scholarly journals Crystal structure of glutamate dehydrogenase 2, a positively selected novel human enzyme involved in brain biology and cancer pathophysiology

2021 ◽  
Author(s):  
Christina Dimovasili ◽  
Vasiliki E. Fadouloglou ◽  
Aikaterini Kefala ◽  
Mary Providaki ◽  
Dina Kotsifaki ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Glutamate Dehydrogenase ◽  
Human Enzyme

Crystal structure of glutamate dehydrogenase 3 from Candida albicans

2021 ◽  
Vol 570 ◽  
pp. 15-20
Author(s):  
Na Li ◽  
Wenfeng Wang ◽  
Xue Zeng ◽  
Mingjie Liu ◽  
Mengyu Li ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Candida Albicans ◽  
Glutamate Dehydrogenase

scholarly journals Crystal structure of the 2-iminoglutarate-bound complex of glutamate dehydrogenase fromCorynebacterium glutamicum

FEBS Letters ◽  
2017 ◽  
Vol 591 (11) ◽  
pp. 1611-1622 ◽  
Author(s):  
Takeo Tomita ◽  
Lulu Yin ◽  
Shugo Nakamura ◽  
Saori Kosono ◽  
Tomohisa Kuzuyama ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Glutamate Dehydrogenase

The First Crystal Structure of Hyperthermostable NAD-dependent Glutamate Dehydrogenase from Pyrobaculum islandicum

2005 ◽  
Vol 345 (2) ◽  
pp. 325-337 ◽  
Author(s):  
Mohammad W. Bhuiya ◽  
Haruhiko Sakuraba ◽  
Toshihisa Ohshima ◽  
Takahito Imagawa ◽  
Nobuhiko Katunuma ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Glutamate Dehydrogenase ◽  
Pyrobaculum Islandicum
Sign in / Sign up

Export Citation Format

Share Document