Multifocal two-photon laser scanning microscopy combined with photo-activatable GFP for in vivo monitoring of intracellular protein dynamics in real time

2007 ◽  
Vol 158 (3) ◽  
pp. 401-409 ◽  
Author(s):  
Joerg Martini ◽  
Katja Schmied ◽  
Ralf Palmisano ◽  
Katja Toensing ◽  
Dario Anselmetti ◽  
...  
Keyword(s):  
Real Time ◽  
Protein Dynamics ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Intracellular Protein ◽  
In Vivo Monitoring ◽  
Two Photon

scholarly journals CdSe/CdS-quantum rods: fluorescent probes for in vivo two-photon laser scanning microscopy

Nanoscale ◽  
2014 ◽  
Vol 6 (17) ◽  
pp. 10413-10422 ◽  
Author(s):  
Jelena Dimitrijevic ◽  
Lisa Krapf ◽  
Christopher Wolter ◽  
Christian Schmidtke ◽  
Jan-Philip Merkl ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Laser Scanning ◽  
Aqueous Media ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Cds Quantum Dots ◽  
Two Photon ◽  
Quantum Rods ◽  
And Performance

CdSe/CdS-Quantum-dots-quantum-rods are encapsulated by PI-b-PEG shells and transferred into various aqueous media to study their stability and performance as probes for two-photon laser scanning microscopy.

scholarly journals In VivoTime-Course Imaging of Tumor Angiogenesis in Colorectal Liver Metastases in the Same Living Mice Using Two-Photon Laser Scanning Microscopy

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Koji Tanaka ◽  
Yuhki Morimoto ◽  
Yuji Toiyama ◽  
Kohei Matsushita ◽  
Mikio Kawamura ◽  
...  
Keyword(s):  
Time Series ◽  
Cancer Cells ◽  
Tumor Angiogenesis ◽  
Laser Scanning ◽  
Fluorescent Protein ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Interindividual Variation ◽  
Two Photon

In vivoreal-time visualization of the process of angiogenesis in secondary tumors in the same living animals presents a major challenge in metastasis research. We developed a technique for intravital imaging of colorectal liver metastasis development in live mice using two-photon laser scanning microscopy (TPLSM). We also developed time-series TPLSM in which intravital TPLSM procedures were performed several times over periods of days to months. Red fluorescent protein-expressing colorectal cancer cells were inoculated into the spleens of green fluorescent protein-expressing mice. First- and second-round intravital TPLSM allowed visualization of viable cancer cells (red) in hepatic sinusoids or the space of Disse. Third-round intravital TPLSM demonstrated liver metastatic colonies consisting of viable cancer cells and surrounding stroma with tumor vessels (green).In vivotime-course imaging of tumor angiogenesis in the same living mice using time-series TPLSM could be an ideal tool for antiangiogenic drug evaluation, reducing the effects of interindividual variation.

scholarly journals Optimizing pulse compressibility in completely all-fibered Ytterbium chirped pulse amplifiers for in vivo two photon laser scanning microscopy

2017 ◽  
Vol 8 (8) ◽  
pp. 3526 ◽  
Author(s):  
A. Fernández ◽  
L. Grüner-Nielsen ◽  
M. Andreana ◽  
M. Stadler ◽  
S. Kirchberger ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Chirped Pulse ◽  
Two Photon ◽  
Chirped Pulse Amplifiers

scholarly journals In vivo high-resolution structural imaging of large arteries in small rodents using two-photon laser scanning microscopy

2010 ◽  
Vol 15 (1) ◽  
pp. 011108 ◽  
Author(s):  
Remco T. A. Megens ◽  
Sietze Reitsma ◽  
Lenneke Prinzen ◽  
Mirjam G. A. oude Egbrink ◽  
Wim Engels ◽  
...  
Keyword(s):  
High Resolution ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Structural Imaging ◽  
Small Rodents ◽  
Large Arteries ◽  
Two Photon
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