Immunogold localisation of microcystins in cryosectioned cells of Microcystis

2005 ◽  
Vol 151 (2) ◽  
pp. 208-214 ◽  
Author(s):  
Fiona M. Young ◽  
Calum Thomson ◽  
James S. Metcalf ◽  
John M. Lucocq ◽  
Geoffrey A. Codd
Keyword(s):  
Immunogold Localisation

Immunogold localisation of endogenous immunoglobulin-G in ultrathin frozen sections of the human placenta

1989 ◽  
Vol 257 (3) ◽  
pp. 603-607 ◽  
Author(s):  
Lopa Leach ◽  
Bryan M. Eaton ◽  
J. Anthony Firth ◽  
Soli F. Contractor
Keyword(s):  
Human Placenta ◽  
Immunoglobulin G ◽  
Frozen Sections ◽  
Immunogold Localisation ◽  
Ultrathin Frozen Sections

Endometrial modifications during early pregnancy in bonnet monkeys (Macaca radiata)

2008 ◽  
Vol 20 (2) ◽  
pp. 281 ◽  
Author(s):  
Gracy X. Rosario ◽  
Serena J. D'Souza ◽  
Dhananjay D. Manjramkar ◽  
Vipul Parmar ◽  
Chander P. Puri ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Early Pregnancy ◽  
Oestrogen Receptor ◽  
Glandular Epithelium ◽  
Luminal Epithelium ◽  
Macaca Radiata ◽  
Transmission Electron ◽  
Immunogold Localisation ◽  
Distinct Features

The present study was undertaken to investigate endometrial modifications that occur before embryo invasion in bonnet monkeys (Macaca radiata). These changes were analysed in luminal epithelium, glandular epithelium and stroma of endometrial functionalis on Day 6 post ovulation from pregnant and non-pregnant animals (n = 4 each) by transmission electron microscopy. Distinct features (i.e. loss of columnar shape by epithelial cells, changes in mitochondrial size and diffused apicolateral gap junctions) were observed in the luminal and glandular epithelium in pregnant animals. Stromal compaction was also observed in pregnant animals. Further, immunogold localisation studies demonstrated significantly higher expression (P < 0.05) of oestrogen receptor α, an oestrogen-regulated gene, in the glandular epithelium and stroma of the endometrium in pregnant animals compared with non-pregnant animals. Expression of two other genes known to be regulated by oestradiol, namely β-actin and cyclo-oxygenase-1, were also significantly higher (P < 0.05) in the endometria of pregnant animals. These studies demonstrate marked changes in the endometrium before embryo invasion in bonnet monkeys. These studies also indicate altered oestrogenic activity in the uterine milieu before embryo invasion.

scholarly journals Spatiotemporal Changes in Xylan-1/Xyloglucan and Xyloglucan Xyloglucosyl Transferase (XTH-Xet5) as a Step-In of Ultrastructural Cell Wall Remodelling in Potato–Potato Virus Y (PVYNTN) Hypersensitive and Susceptible Reaction

2018 ◽  
Vol 19 (8) ◽  
pp. 2287 ◽  
Author(s):  
Katarzyna Otulak-Kozieł ◽  
Edmund Kozieł ◽  
Józef Bujarski
Keyword(s):  
Cell Wall ◽  
Molecular Mechanisms ◽  
Hypersensitive Reaction ◽  
Stress Factors ◽  
Susceptible Host ◽  
Immunogold Localisation ◽  
Defence Responses ◽  
Controlled Structure ◽  
Virus Interactions ◽  
Golgi Network

One type of monitoring system in a plant cell is the cell wall, which intensively changes its structure during interaction with pathogen-stress factors. The wall plays a role as a dynamic and controlled structure, although it is not fully understood how relevant these modifications are to the molecular mechanisms during plant–virus interactions. In this work we localise the non-cellulosic polysaccharides such as xyloglucan, xylan (xylan-1) and xyloglucosyl transferase (XTH-Xet5), the enzyme that participates in the metabolism of xyloglucan. This provided us with information about the in situ distribution of the components of the hemicellulotic cell wall matrix in hypersensitive and susceptible potato–PVYNTN interactions. The loosening of the cell wall was accompanied by an increase in xylan depositions during susceptible interactions, whereas, during the hypersensitive response, when the cell wall was reinforced, the xylan content decreased. Moreover, the PVY inoculation significantly redirected XTH-Xet5 depositions, regardless of types of interactions, compared to mock-inoculated tissues. Furthermore, the immunogold localisation clearly revealed the domination of Xet5 in the cell wall and in vesicles in the susceptible host. In contrast, in the resistant host increased levels of Xet5 were observed in cytoplasm, in the cell wall and in the trans-Golgi network. These findings show that the hypersensitive reaction activated XTH-Xet5 in the areas of xyloglucan endo-transglycosylase (XET) synthesis, which was then actively transported to cytoplasm, cell wall and to vacuoles. Our results provide novel insight into cell wall reorganisation during PVYNTN infection as a response to biotic stress factors. These novel findings help us to understand the mechanisms of defence responses that are incorporated into the cell wall signalling network.

scholarly journals Immunogold localisation of laminin in normal and exfoliative iris.

1990 ◽  
Vol 74 (8) ◽  
pp. 450-457 ◽  
Author(s):  
A. G. Konstas ◽  
G. E. Marshall ◽  
W. R. Lee
Keyword(s):  
Immunogold Localisation

Immunogold localisation of hemoglobin inCasuarina root nodules

PROTOPLASMA ◽  
1997 ◽  
Vol 198 (3-4) ◽  
pp. 130-134 ◽  
Author(s):  
D. J. Goodchild ◽  
C. Miller
Keyword(s):  
Root Nodules ◽  
Immunogold Localisation
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