scholarly journals Lipid domain formation and dynamics in giant unilamellar vesicles explored by fluorescence correlation spectroscopy

2004 ◽  
Vol 147 (1) ◽  
pp. 77-89 ◽  
Author(s):  
Nicoletta Kahya ◽  
Dag Scherfeld ◽  
Kirsten Bacia ◽  
Petra Schwille
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Domain Formation ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Lipid Domain ◽  
Fluorescence Correlation

Confidence Intervals for Estimation of the Concentration and Brightness of Multiple Diffusing Species

2012 ◽  
Author(s):  
Xi Rong ◽  
Kenneth M. Pryse ◽  
Jordan A. Whisler ◽  
Yanfei Jiang ◽  
William B. McConnaughey ◽  
...  
Keyword(s):  
Confidence Intervals ◽  
Significant Role ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Membrane Rafts ◽  
Giant Unilamellar Vesicles ◽  
Enveloped Viruses ◽  
Fluorescence Correlation ◽  
Pathological Conditions ◽  
Signaling Mechanisms

Lipid nanodomains in cell membranes are believed to play a significant role in replication of enveloped viruses such as bird flu and HIV and signaling mechanisms underlying pathological conditions such as cancer. However, the forces that govern the formation and availability of these “membrane rafts” are uncertain, and even their existence is questioned. The central challenge is that no suitable imaging modalities exist (Elson, et al., 2010). We are developing tools to characterize and visualize dynamics of lipid nanodomains on idealized systems called giant unilamellar vesicles (GUVs) using fluorescence correlation spectroscopy (FCS) (Figure 1).

scholarly journals Nano-antenna enhanced two-focus fluorescence correlation spectroscopy

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Lutz Langguth ◽  
Agata Szuba ◽  
Sander A. Mann ◽  
Erik C. Garnett ◽  
Gijsje H. Koenderink ◽  
...  
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation
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