scholarly journals Inflammation influences steroid hormone receptors targeted by progestins in endometrial stromal cells from women with endometriosis

2016 ◽  
Vol 117 ◽  
pp. 30-38 ◽  
Author(s):  
Giovanni Grandi ◽  
Michael D. Mueller ◽  
Andrea Papadia ◽  
Vida Kocbek ◽  
Nick A. Bersinger ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Hormone Receptors ◽  
Steroid Hormone ◽  
Steroid Hormone Receptors ◽  
Endometrial Stromal Cells

Immunolocalization of sex steroid hormone receptors in the canine uterine tube and their relation to sex steroid hormone levels

2002 ◽  
Vol 14 (4) ◽  
pp. 241 ◽  
Author(s):  
Hilde Vermeirsch ◽  
Wim Van Den Broeck ◽  
Mark Coryn ◽  
Paul Simoens
Keyword(s):  
Epithelial Cells ◽  
Estrous Cycle ◽  
Stromal Cells ◽  
Hormone Receptors ◽  
Steroid Hormone ◽  
Steroid Hormone Receptors ◽  
Sex Steroid ◽  
Nuclear Staining ◽  
Sex Steroid Hormone ◽  
Uterine Tube

The aim of this immunohistochemical study was to describe the cellular distribution of the estrogen receptor-α (ERα), progesterone receptor (PR) and androgen receptor (AR) in canine uterine tubes. Samples of uterine tubes were taken from dogs in different stages of the estrous cycle, and dogs that were pregnant or had just delivered. Nuclear staining for sex steroid hormone receptors was observed in the surface epithelium, stromal cells and smooth muscle cells of the muscular layer. Only slight differences in staining pattern were observed between the ampulla and fimbriae. The staining for ERα and PR showed changes throughout the estrous cycle. Some of these changes were related to changing concentrations of sex steroid hormones. High staining scores for ERα and PR were found during proestrus and low scores during early metestrus. The staining for AR showed only minor cyclic changes. However, during proestrus and estrus, cytoplasmic staining for AR was observed in differentiated secretory epithelial cells, when nuclear staining in these cells was nearly absent. For the three hormone receptors, stromal cells generally stained with a higher intensity than epithelial cells. It is likely that many steroid hormone actions on the epithelium are mediated through stromal cells. During pregnancy, rather high staining scores were found for ERα and AR in the uterine tube. This is in contrast to observations in the canine pregnant uterus.

scholarly journals Ontogeny and expression profiles of steroid hormone receptors in a mouse model of endometriosis

2019 ◽  
Author(s):  
Anuradha Mishra ◽  
Mosami Galvankar ◽  
Neha Singh ◽  
Shantashri Vaidya ◽  
Uddhav Chaudhari ◽  
...  
Keyword(s):  
Mouse Model ◽  
Stromal Cells ◽  
Hormone Receptors ◽  
Steroid Hormone ◽  
Expression Profiles ◽  
Steroid Hormone Receptors ◽  
Reproductive Age ◽  
Unknown Etiology ◽  
Mrna Levels ◽  
Post Surgery

ABSTRACTEndometriosis is a chronic incurable disorder of unknown etiology affecting a large proportion of women in reproductive age. In order to understand the pathogenesis and preclinical testing of drugs,animal models that recapitulate the key features of the disorder are highly desirous. Herein, we describe the ontogeny of the ectopic endometrial lesion in a mouse model where uterine tissue was ligated to the intestinal mesentery and the animals were followed up from day 5 to day 60 post-surgery. Out of 60 animals that underwent surgery, 58 developed endometriosis using this strategy. Most lesions were pale, fluid filled while red lesions were seen in ~10% of animals. Histologically, in most animals there was one large cystic gland with well differentiated epithelium, in 13% of animals there was mixed phenotype (well and poorly differentiated). There was extensive stromal compaction and increased number of macrophages in ectopic lesions. During the course of endometriosis, there was an increase in number of PCNA positive epithelial and stromal cells. The epithelial cells at all the time point were cytokeratin positive and the stroma was vimentin positive. However, at day 30 and 60, the stromal cells were also cytokeratin positive. The mRNA levels of estrogen receptorsEsr1andGper1were reduced while those ofEsr2were elevated as compared to normal endometrium, the levels of progesterone receptor (Pgr) were found to be downregulated in ectopic lesions as compared to control. However, these differences were not statistically significant due to high biological variability. Low abundance ofCyp19a1transcripts (aromatase gene) were only detected in the ectopic endometrium. Immunohistochemically, the expression of ERα and ERβ was significantly reduced only in stromal cells; the epithelial cell staining was maintained. GPER1 and PR immunoreactivity was significantly low in both epithelial and stromal cells. The immunostaining of all the steroid receptors was highly heterogeneous in the ectopic tissues with some areas of sections had stained intensely while others had negligible staining. We propose that temporal and spatial difference in the expression of steroid hormone receptors during the course of endometriosis development coupled with micro-heterogeneity may alter the effectiveness of steroid hormone analogues resulting in variable outcomes and often failure of therapy.

Can hormone receptors prevent metastatic spread of gastric cancer?

2019 ◽  
Vol 37 (15_suppl) ◽  
pp. e15527-e15527
Author(s):  
Irina V. Kaplieva ◽  
Oleg I. Kit ◽  
Elena M. Frantsiyants ◽  
Valeria A. Bandovkina ◽  
Yuriy A. Gevorkyan ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Extracellular Matrix ◽  
Immune Cells ◽  
Stromal Cells ◽  
Hormone Receptors ◽  
Comparison Group ◽  
Steroid Hormone ◽  
Steroid Hormone Receptors ◽  
Greater Omentum ◽  
Control Group

e15527 Background: The primary tumor and associated but distinct from it premetastatic niches include a number of important participants: immune cells, stromal cells, extracellular matrix and associated effectors, in particular hormone receptors. Cross-interaction between these components is key to tumor progression. The greater omentum and peritoneum are pre-metastatic niches for gastric cancer. The purpose of the study was to analyze levels of steroid hormone receptors in tissues of gastric cancer (GC) and its pre-metastatic niches: the peritoneum (P) and omentum (O). Methods: The main group included 21 patients with metastatic GC T3-4аN0-3M1; the comparison group – 24 patients with non-metastatic GC T3-4аN0-3M0. Tissues of tumors, intact gastric tissues, omentum and peritoneum tissues were studied. The control group included 17 non-cancer patients; omental and peritoneal tissues were studied. Levels of receptors of estrogens (RE-α, RE-β), androgens (RA) and progesterone (RP4) were measured by ELISA. Results: Levels of RE-α in tissue of GC T3-4аN0-3M1 were decreased by 1.7 times (P˂0.05) compared to controls, while in T3-4аN0-3M0 they were increased by 1.2 times (P˂0.05). RE-β and RА did not change in both cases. RР4 in T3-4аN0-3M1 was similar to control levels, and in T3-4аN0-3M0 – was increased by 3.5 times. Levels of RE-α and β, RА and RР4 in O and P in T3-4аN0-3M1 were similar to control levels, and in T3-4аN0-3M0 they were higher: RE-α - by 3.9 and 2.4 times, RE-β – by 2.5 and 1.5 times (P˂0.05), RР4 – by 2.2 and 1.5 times (P˂0.05). RA in O and P in T3-4аN0-3M0 was similar to control levels. Conclusions: Elevated levels of RE-α., RE-β and RR4 in peritoneal and omental tissues can be considered a factor associated with some characteristics of GC metastasis, and protective one at the same time. Obviously, levels of RE-α and RР in O and P correlate with the levels in tissues of GC T3-4аN0-3M0. However, this statement is disputable and requires further confirmation.

Sign in / Sign up

Export Citation Format

Share Document