Crucial role of hypoxia inducible factor in embryo implantation

2015 ◽  
Vol 112 ◽  
pp. 124
Author(s):  
Leona Matsumoto ◽  
Yasushi Hirota ◽  
Tomoko Saito-Fujita ◽  
Hirofumi Haraguchi ◽  
Mahiro Egashira ◽  
...  
Keyword(s):  
Crucial Role ◽  
Embryo Implantation ◽  
Hypoxia Inducible Factor

Stathmin 1 plays a role in endometrial decidualisation by regulating hypoxia inducible factor-1α and vascular endothelial growth factor during embryo implantation

2017 ◽  
Vol 29 (8) ◽  
pp. 1530 ◽  
Author(s):  
Jinhai Gou ◽  
Jia Jia ◽  
Juntao Feng ◽  
Xia Zhao ◽  
Tao Yi ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Embryo Implantation ◽  
Hypoxia Inducible Factor ◽  
Implantation Site ◽  
Vascular Endothelial ◽  
Stathmin 1 ◽  
Endothelial Growth Factor

The aim of the present study was to explore the potential mechanism underlying stathmin 1 (Stmn1) regulation of embryo implantation, as a continuation of previous proteomic research. Adult healthy female mice were mated naturally with fertile males. Murine uterine tissue was collected during the peri-implantation period. Local expression of Stmn1 during embryo implantation was detected by immunohistochemistry (IHC), which showed that Stmn1 was extensively expressed in endometrial glandular epithelium, vascular endothelium, luminal epithelium and the underlying stromal cells at the implantation site on Day 5. The role of Stmn1 during embryo implantation was evaluated by transient knockdown of Stmn1 in vivo using short interference (si) RNA, and some associated factors including Akt, phosphorylated (p-) Akt, hypoxia-inducible factor (HIF)-1α, prolactin (PRL), insulin-like growth factor binding protein (IGFBP) 1 and vascular endothelial growth factor (VEGF) were examined by western blotting analysis and ELISA. The number of embryos implanted after Stmn1-siRNA infusion into the lumen of one uterine horn was lower than that with normal pregnancies (2.2 ± 1.5 vs 8.6 ± 0.5 respectively; P < 0.05). The expression of VEGF, HIF-1α, p-Akt and the decidualisation biomarkers PRL and IGFBP 1 was upregulated at the implantation site on Day 5, but downregulated after Stmn1-siRNA infusion. These findings suggest that during embryo implantation, knockdown of Stmn1 suppresses decidualisation by inhibiting the expression of p-Akt, HIF-1α and VEGF, thus leading to impaired embryo implantation. These findings provide clues for understanding the complicated process of embryo implantation and the potential role of Stmn1 during embryo implantation.

scholarly journals Hypoxia-Inducible Factor 1-Regulated Lysyl Oxidase Is Involved in Staphylococcus aureus Abscess Formation

2013 ◽  
Vol 81 (7) ◽  
pp. 2562-2573 ◽  
Author(s):  
Christiane Beerlage ◽  
Jessica Greb ◽  
Dorothee Kretschmer ◽  
Mohammad Assaggaf ◽  
Philip C. Trackman ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Crucial Role ◽  
Lysyl Oxidase ◽  
Bacterial Load ◽  
Hypoxia Inducible Factor ◽  
Abscess Formation ◽  
Content Type ◽  
Hypoxia Inducible Factor 1 ◽  
Extracellular Matrix Components

ABSTRACTHypoxia-inducible factor 1 (HIF-1) is the key transcription factor involved in the adaptation of mammals to hypoxia and plays a crucial role in cancer angiogenesis. Recent evidence suggests a leading role for HIF-1 in various inflammatory and infectious diseases. Here we describe the role of HIF-1 inStaphylococcus aureusinfections by investigating the HIF-1-dependent host cell response. For this purpose, transcriptional profiling of HIF-1α-deficient HepG2 and control cells, both infected withStaphylococcus aureus, was performed. Four hours after infection, the expression of 190 genes, 24 of which were regulated via HIF-1, was influenced.LOX(encoding lysyl oxidase) was one of the upregulated genes with a potential impact on the course ofS. aureusinfection. LOX is an amine oxidase required for biosynthetic cross-linking of extracellular matrix components. LOX was upregulatedin vitroin different cell cultures infected withS. aureusand alsoin vivo, in kidney abscesses of mice intravenously infected withS. aureusand in clinical skin samples from patients withS. aureusinfections. Inhibition of LOX by β-aminopropionitrile (BAPN) did not affect the bacterial load in kidneys or blood but significantly influenced abscess morphology and collagenization. Our data provide evidence for a crucial role of HIF-1-regulated LOX in abscess formation.

scholarly journals Role of extracellular signal-regulated kinase and AKT cascades in regulating hypoxia-induced angiogenic factors produced by a trophoblast-derived cell line

2010 ◽  
Vol 206 (1) ◽  
pp. 131-140 ◽  
Author(s):  
Daisuke Fujita ◽  
Akiko Tanabe ◽  
Tatsuharu Sekijima ◽  
Hekiko Soen ◽  
Keijirou Narahara ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Kinase Inhibitors ◽  
Transforming Growth Factor ◽  
Embryo Implantation ◽  
Hypoxia Inducible Factor ◽  
Transforming Growth Factor Β1 ◽  
Angiogenic Factors ◽  
Placental Development

During human pregnancy, trophoblasts play an important role in embryo implantation and placental development. Cytotrophoblast cells invade the uterine spiral arteries and differentiate into extravillous trophoblasts, resulting in the remodeling of the uterine vessels and fetoplacental vasculature. During early pregnancy, a physiologically hypoxic environment induces the production of angiogenic factors, such as vascular endothelial growth factor (VEGF), which are suggested to locally control the vascular remodeling. Endoglin, a cell-surface coreceptor for transforming growth factor-β1, is highly expressed in endothelial cells and syncytiotrophoblasts, and can be associated with endothelial nitric oxide synthase and vascular homeostasis. Several studies have recently suggested that some pregnancy-related complications, such as preeclampsia, have their origins early in pregnancy as a result of abnormalities in implantation and placental development. Although angiogenic factors are recognized as key molecules in placental development, little is known about the mechanism(s) of their regulation in trophoblasts. In this study, we elucidated the mechanisms underlying the regulation of VEGF and endoglin production under hypoxic conditions in the trophoblast-derived cell line, BeWo. We evaluated the role of the AKT–MTOR cascade and ERK kinase in the expression of VEGF and endoglin in response to hypoxia using various kinase inhibitors and small interfering RNA targeted against hypoxia-inducible factor (HIF)-1α (listed as HIF1A in Hugo Database). Our results suggest that both the phosphatidylinositol 3-kinase–AKT–MTOR–HIF-1α and ERK–HIF-1α signaling pathways are crucial for increasing VEGF and endoglin expression in response to hypoxia in BeWo cells.

scholarly journals The Role of Hypoxia-Inducible Factor Post-Translational Modifications in Regulating its Localisation, Stability and Activity

2020 ◽  
Author(s):  
Adam Albanese ◽  
Leonard A. Daly ◽  
Daniela Mennerich ◽  
Thomas Kietzmann ◽  
Violaine Sée
Keyword(s):  
Transcription Factors ◽  
Crucial Role ◽  
Hypoxia Inducible Factor ◽  
Oxygen Availability ◽  
Signalling Pathway ◽  
Hypoxia Inducible Factors ◽  
Cellular Homeostasis ◽  
Post Translational Modifications ◽  
Alpha Subunits

The hypoxia signalling pathway enables adaptation of cells to decreased oxygen availability. When oxygen becomes limiting, the central transcription factors of the pathway, hypoxia-inducible factors (HIFs), are stabilised and activated to induce the expression of hypoxia-regulated genes, thereby maintaining cellular homeostasis. Whilst hydroxylation has been thoroughly described as the major and canonical modification of the HIF-&alpha; subunits, regulating both HIF stability and activity, a range of other post-translational modifications decorating the entire protein play also a crucial role in altering HIF localisation, stability, and activity. These modifications, their conservation throughout evolution and their effects on HIF-dependent signalling are discussed in this review.

scholarly journals The Role of Hypoxia-Inducible Factor Post-Translational Modifications in Regulating Its Localisation, Stability, and Activity

2020 ◽  
Vol 22 (1) ◽  
pp. 268
Author(s):  
Adam Albanese ◽  
Leonard A. Daly ◽  
Daniela Mennerich ◽  
Thomas Kietzmann ◽  
Violaine Sée
Keyword(s):  
Transcription Factors ◽  
Crucial Role ◽  
Hypoxia Inducible Factor ◽  
Oxygen Availability ◽  
Signalling Pathway ◽  
Hypoxia Inducible Factors ◽  
Cellular Homeostasis ◽  
Post Translational Modifications ◽  
Α Subunits

The hypoxia signalling pathway enables adaptation of cells to decreased oxygen availability. When oxygen becomes limiting, the central transcription factors of the pathway, hypoxia-inducible factors (HIFs), are stabilised and activated to induce the expression of hypoxia-regulated genes, thereby maintaining cellular homeostasis. Whilst hydroxylation has been thoroughly described as the major and canonical modification of the HIF-α subunits, regulating both HIF stability and activity, a range of other post-translational modifications decorating the entire protein play also a crucial role in altering HIF localisation, stability, and activity. These modifications, their conservation throughout evolution, and their effects on HIF-dependent signalling are discussed in this review.

Epitaxial YBa2Cu3O7-8 films : Crucial role of growth-induced linear defects in microwave properties

2001 ◽  
Vol 11 (PR11) ◽  
pp. Pr11-47-Pr11-52
Author(s):  
V. M. Pan ◽  
V. S. Flis ◽  
V. A. Komashko ◽  
O. G. Plys ◽  
C. G. Tretiatchenko ◽  
...  
Keyword(s):  
Crucial Role ◽  
Microwave Properties ◽  
Linear Defects
Sign in / Sign up

Export Citation Format

Share Document