Whole blood flow cytometric analysis of Ureaplasma-stimulated monocytes from pregnant women

2015 ◽  
Vol 109 ◽  
pp. 84-88 ◽  
Author(s):  
Yael D. Friedland ◽  
Tracey F. Lee-Pullen ◽  
Elizabeth Nathan ◽  
Rory Watts ◽  
Jeffrey A. Keelan ◽  
...  
Keyword(s):  
Blood Flow ◽  
Pregnant Women ◽  
Whole Blood ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric

scholarly journals Whole-Blood Flow-Cytometric Analysis of Antigen-Specific CD4 T-Cell Cytokine Profiles Distinguishes Active Tuberculosis from Non-Active States

PLoS ONE ◽  
2011 ◽  
Vol 6 (3) ◽  
pp. e17813 ◽  
Author(s):  
Urban Sester ◽  
Mathias Fousse ◽  
Jan Dirks ◽  
Ulrich Mack ◽  
Antje Prasse ◽  
...  
Keyword(s):  
Blood Flow ◽  
T Cell ◽  
Whole Blood ◽  
Flow Cytometric Analysis ◽  
Active Tuberculosis ◽  
Cd4 T Cell ◽  
Cytokine Profiles ◽  
Flow Cytometric

Whole-Blood Flow-Cytometric Analysis of Eosinophil EG2 Expression as a Marker of the Pathological Conditions of Asthma

1998 ◽  
Vol 117 (1) ◽  
pp. 77-80 ◽  
Author(s):  
Tomoe Kuwasaki ◽  
Junichi Chihara ◽  
Hiroyuki Kayaba ◽  
Yumiko Kamata ◽  
Hazime Oyamada ◽  
...  
Keyword(s):  
Blood Flow ◽  
Whole Blood ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Pathological Conditions

Whole-Blood Flow-Cytometric Analysis of Induction of Adhesion Molecules Expression on Eosinophils Stimulated by Phorbol-12-Myristate-13-Acetate/Ionomycin

1999 ◽  
Vol 120 (1) ◽  
pp. 27-29 ◽  
Author(s):  
Norihiro Saito ◽  
Hiroyuki Kayaba ◽  
Kohei Honda ◽  
Yoshiyuki Yamada ◽  
Chang-Hao Cui ◽  
...  
Keyword(s):  
Blood Flow ◽  
Adhesion Molecules ◽  
Whole Blood ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric

Flow Cytometric Detection of Activated Platelets in Pregnant Women Prior to the Development of Pre-Eclampsia

1995 ◽  
Vol 74 (04) ◽  
pp. 1059-1063 ◽  
Author(s):  
Sarah L Janes ◽  
Phillipa M Kyle ◽  
Christopher Redman ◽  
Alison H Goodall
Keyword(s):  
Blood Flow ◽  
Pregnant Women ◽  
Whole Blood ◽  
Ex Vivo ◽  
Critical Role ◽  
Antigen Expression ◽  
Normal Pregnancy ◽  
Flow Cytometric Method ◽  
Flow Cytometric ◽  
Activated Platelets

SummaryPre-eclampsia is a common complication of pregnancy, in which platelets may have an early pathogenetic role. In this prospective study a whole blood flow cytometric method has been used to detect circulating activated platelets in pregnant women prior to the development of pre-eclampsia. Activated platelets were identified by bound fibrinogen or by CD63 antigen expression. Of 121 healthy primiparous women studied at 28 weeks of pregnancy, 18 (15%) developed clinical pre-eclampsia six to thirteen weeks later. The platelets of these women showed increased fibrinogen binding ex vivo (5.1% platelets positive, compared with 3.4% in those who completed a normal pregnancy, p <0.02), and increased CD63 antigen expression (0.73% positive compared to 0.45%, p = 0.01). In contrast, no differences between the women with different outcomes were detected at 28 weeks in platelet counts, or plasma ß-thromboglobulin levels. These findings confirm that whole blood flow cytometry is a sensitive technique for investigating platelet activation in a clinical setting and support the hypothesis that platelets have a critical role in the pathogenesis of pre-eclampsia

Evaluation of Whole Blood Flow Cytometric Detection of Platelet Bound Fibrinogen on Normal Subjects and Patients with Activated Platelets

1993 ◽  
Vol 70 (04) ◽  
pp. 659-666 ◽  
Author(s):  
Sarah L Janes ◽  
Darren J Wilson ◽  
Nicolas Chronos ◽  
Alison H Goodall
Keyword(s):  
Blood Flow ◽  
Whole Blood ◽  
Flow Cytometric Analysis ◽  
Normal Subjects ◽  
Cell Counting ◽  
Clinical Samples ◽  
Median Percentage ◽  
Flow Cytometric ◽  
Activated Platelets ◽  
Fibrinogen Binding

SummaryActivated platelets can be detected by measuring platelet-bound fibrinogen in a whole blood, flow cytometric assay, using a fluorescently-conjugated polyclonal antibody.Fibrinogen binding to unstimulated platelets from normal subjects was low in this assay, as was expression of the CD63 antigen. Single cell counting of samples prepared for flow cytometric analysis showed platelet aggregates do not form during the assay procedure. Immune complexes were not seen, and fibrinogen binding to the platelets was unaffected by the CD32 MAb, IV.3. Artefactual activation of the unfixed samples could be minimised by control of phlebotomy, time and temperature of incubation. Variations in platelet count in the range 140–430 × 109 1-1 and in plasma fibrinogen in the range 2–6 g 1-1 did not affect the assay results.Comparison of fibrinogen binding with expression of CD63 antigen on normal platelets, stimulated with agonists in vitro, demonstrated that fibrinogen binding detects an earlier stage of platelet activation.Platelet bound fibrinogen was shown to be sensitive in detecting small numbers of activated platelets in clinical samples in twelve patients on intensive care, four undergoing haemofiltration. The patients had a significantly higher median percentage of circulating platelets with bound fibrinogen (p <0.005), but fibrinogen binding was significantly lower (p <0.02) in response to 10-5 M ADP, compared to twelve age-matched normal Controls.

HLA class I expression on the erythrocytes of cord and maternal blood — Flow cytometric analysis

1996 ◽  
Vol 47 (1-2) ◽  
pp. 110
Author(s):  
G. Paterakis ◽  
A. Germenis ◽  
D. Skoumi ◽  
N. Koutsodimas ◽  
C. Stavropoulos-Giokas
Keyword(s):  
Blood Flow ◽  
Flow Cytometric Analysis ◽  
Hla Class I ◽  
Maternal Blood ◽  
Class I ◽  
Flow Cytometric
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