Heat shock protein 70 (HSP70) expression in uNK cells of normal pregnant mice uterus and under stress induced by embryo lesion

2007 ◽  
Vol 75 (1) ◽  
pp. A13
Author(s):  
P.D.A. Lima ◽  
A.T. Yamada
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Hsp70 Expression ◽  
Unk Cells ◽  
Pregnant Mice

scholarly journals Effects ofHelicobacter pyloriand Heat Shock Protein 70 on the Proliferation of Human Gastric Epithelial Cells

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Liping Tao ◽  
Hai Zou ◽  
Zhimin Huang
Keyword(s):  
Helicobacter Pylori ◽  
Heat Shock ◽  
Epithelial Cells ◽  
Heat Shock Protein ◽  
Mtt Assay ◽  
Heat Shock Protein 70 ◽  
Hsp70 Expression ◽  
Gastric Epithelial Cells ◽  
Ags Cells ◽  
H Pylori

Infection ofHelicobacter pylori (H. pylori)changed the proliferation of gastric epithelial cells and decreased the expression of heat shock protein 70 (HSP70). However, the effects ofH. pylorion the proliferation of gastric epithelial cells and the roles of HSP70 during the progress need further investigation.Objective.To investigate the effects ofHelicobacter pylori (H. pylori)and heat shock protein 70 (HSP70) on the proliferation of human gastric epithelial cells.Methods. H. pyloriand a human gastric epithelial cell line (AGS) were cocultured. The proliferation of AGS cells was quantitated by an MTT assay, and the expression of HSP70 in AGS cells was detected by Western blotting. HSP70 expression in AGS cells was silenced by small interfering RNA (siRNA) to investigate the role of HSP70. ThesiRNA-treated AGS cells were cocultured withH. pyloriand cell proliferation was measured by an MTT assay.Results.The proliferation of AGS cells was accelerated by coculturing withH. pylorifor 4 and 8 h, but was suppressed at 24 and 48 h. HSP70 expression was decreased in AGS cells infected byH. pylorifor 48 h. The proliferation in HSP70-silenced AGS cells was inhibited after coculturing withH. pylorifor 24 and 48 h compared with the control group.Conclusions.Coculture ofH. pylorialtered the proliferation of gastric epithelial cells and decreased HSP70 expression. HSP70 knockdown supplemented the inhibitory effect ofH. pylorion proliferation of epithelial cells. These results indicate that the effects ofH. pylorion the proliferation of gastric epithelial cells at least partially depend on the decreased expression of HSP70 induced by the bacterium.

Effects of ferulic acid on oxidative stress, heat shock protein 70, connexin 43, and monoamines in the hippocampus of pentylenetetrazole-kindled rats

2017 ◽  
Vol 95 (6) ◽  
pp. 732-742 ◽  
Author(s):  
Abdelaziz M. Hussein ◽  
Khaled M. Abbas ◽  
Osama A. Abulseoud ◽  
El-Hussainy M.A. El-Hussainy
Keyword(s):  
Oxidative Stress ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Ferulic Acid ◽  
Group Treatment ◽  
Heat Shock Protein 70 ◽  
Connexin 43 ◽  
Hsp70 Expression ◽  
Neuroprotective Effects ◽  
Cx43 Expression

The present study investigated the effects of ferulic acid (FA) on pentylenetetrazole (PTZ)-induced seizures, oxidative stress markers (malondialdehyde (MDA), catalase, and reduced glutathione (GSH)), connexin (Cx) 43, heat shock protein 70 (Hsp 70), and monoamines (serotonin (5-HT) and norepinephrine (NE)) levels in a rat model of PTZ-induced kindling. Sixty Sprague Dawley rats were divided into 5 equal groups: (a) normal group; (b) FA group: normal rats received FA at a dose of 40 mg/kg daily; (c) PTZ group: normal rats received PTZ at a dose of 50 mg/kg i.p. on alternate days for 15 days; (d) FA-before group: treatment was the same as for the PTZ group, except rats received FA; and (e) FA-after group: rats received FA from sixth dose of PTZ. PTZ caused a significant increase in MDA, Cx43, and Hsp70 along with a significant decrease in GSH, 5-HT, and NE levels and CAT activity in the hippocampus (p < 0.05). Pre- and post-treatment with FA caused significant improvement in behavioral parameters, MDA, CAT, GSH, 5-HT, NE, Cx43 expression, and Hsp70 expression in the hippocampal region (p < 0.05). We conclude that FA has neuroprotective effects in PTZ-induced epilepsy, which might be due to attenuation of oxidative stress and Cx43 expression and upregulation of neuroprotective Hsp70 and neurotransmitters (5-HT and NE).

scholarly journals The effect of red-fleshed pitaya (Hylocereus polyrhizus) on heat shock protein 70 and cortisol expression in strenuous exercise induced rats

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 130
Author(s):  
Novita Sari Harahap ◽  
Aznan Lelo ◽  
Ambrosius Purba ◽  
Awaluddin Sibuea ◽  
Rina Amelia ◽  
...  
Keyword(s):  
Body Weight ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
The Body ◽  
Male Rats ◽  
Strenuous Exercise ◽  
Hsp70 Expression ◽  
Average Weight ◽  
Significant Difference

Background: Oxidative stress from exercise can contribute to damaging cells, increasing heat shock protein 70 (HSP70) and suppressing the immune system in the body. This research aimed to determine the antioxidant potential of red-fleshed pitaya extract on HSP70 and cortisol expression in rats which were subjected to strenuous exercise. Methods: The subjects of this research were 32 Sprague Dawley male rats, aged 3 months, with an average weight of 200 g. Red-fleshed pitaya extract was obtained from methanol extraction process; a maceration technique was performed and the extract was concentrated using an air-drying method. Rats were randomly divided into four groups. Group 1 were subjected to strenuous exercise and treated with distilled water only; while Groups 2, 3 and 4 were subjected to strenuous exercise and treated with 100 mg/kg body weight, 200 mg/kg body weight and 300 mg/kg body weight of red-fleshed pitaya extract, respectively. Strenuous exercises in rats was performed by intense swimming of 20 min/day, 3 days a week for 3 weeks. HSP70 expression and cortisol were measured with Enzyme-Linked Immune Sorbent Assay (ELISA) method. Results: There was a significant reduction of HSP70 (p=0.000) and cortisol expression (p=0.000) between the groups. Also, there was a significant difference in the average decreasing of HSP70 expression between group 4 and either groups 1 or 2 (p=0.000). However, a significant difference between groups 4 and 3 was not observed (p=0.813). Lastly, a significant difference was found in the average decrease of cortisol expression between groups 4 and 1 (p=0.000), 2 (p=0.000), and 3 (p=0.000) respectively. Conclusion: Red-fleshed pitaya is potential to be utilized as antioxidant to decrease the HSP70 and cortisol expression.

scholarly journals Expression of heat shock protein 70 is insufficient to extend Drosophila melanogaster longevity

2019 ◽  
Author(s):  
Chengfeng Xiao ◽  
Danna Hull ◽  
Shuang Qiu ◽  
Joanna Yeung ◽  
Jie Zheng ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Stress Resistance ◽  
Heat Shock Protein 70 ◽  
Biological Effect ◽  
Hsp70 Expression ◽  
Gene Encoding ◽  
Shock Proteins

AbstractIt has been known for over 20 years that Drosophila melanogaster flies with twelve additional copies of the hsp70 gene encoding the 70 kDa heat shock protein lives longer after a non-lethal heat treatment. Since the heat treatment also induces the expression of additional heat shock proteins, the biological effect can be due either to HSP70 acting alone or in combination. This study used the UAS/GAL4 system to determine whether hsp70 is sufficient to affect the longevity and the resistance to thermal, oxidative or desiccation stresses of the whole organism. We observed that HSP70 expression in the nervous system or muscles has no effect on longevity or stress resistance but ubiquitous expression reduces the life span of males. We also observed that the down-regulation of Hsp70 using RNAi did not affect longevity.

scholarly journals miR-16-1 Promotes the Aberrantα-Synuclein Accumulation in Parkinson Disease via Targeting Heat Shock Protein 70

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Zhelin Zhang ◽  
Yan Cheng
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Parkinson Disease ◽  
Heat Shock Protein 70 ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cell Line ◽  
Hsp70 Expression ◽  
Human Neuroblastoma Cell ◽  
Human Neuroblastoma ◽  
Human Neuroblastoma Cell Line

There is striking evidence that heat shock protein 70 (Hsp70) negatively regulatesα-synuclein aggregation, which plays a significant role in the formation and progression of Parkinson disease (PD). However, how the Hsp70 in neurons fails to prevent or even reverseα-synuclein aggregation and toxicity in PD still remains to be determined. In the present study, we constructed anα-synuclein-overexpressed human neuroblastoma cell line, SH-SY5Y-Syn, in which the blockage of Hsp70 promotedα-synuclein aggregation. And we also found that miR-16-1 downregulated Hsp70 and promotedα-synuclein aggregation in the SH-SY5Y-Syn cells. This study revealed a novel regulatory mechanism of Hsp70 expression, which might contribute to the PD development.

Exogenous heat shock protein-70 inhibits cigarette smoke-induced intimal thickening

2008 ◽  
Vol 295 (4) ◽  
pp. R1320-R1327 ◽  
Author(s):  
Michiaki Matsumoto ◽  
Paul C. Dimayuga ◽  
Charles Wang ◽  
Jonathan Kirzner ◽  
Miha Cercek ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Cigarette Smoke ◽  
Heat Shock Protein 70 ◽  
Nuclear Antigen ◽  
Cigarette Smoke Exposure ◽  
Hsp70 Expression ◽  
Intimal Thickening ◽  
Hsp70 Mrna ◽  
Recombinant Hsp70

Cigarette smoke is associated with increased carotid intimal thickening or stroke. Preliminary work showed that exposure to smoke resulted in a 4.5-fold reduction of heat shock protein-70 (HSP70) expression in spleens of mice using gene microarray analysis. In the current study, we investigated the role of extracellular HSP70 in carotid intimal thickening of mice exposed to cigarette smoke. Intimal thickening was induced by placement of a cuff around the right carotid artery of mice. Cuff injury resulted in increased HSP70 mRNA expression in carotid arteries that persisted for 21 days. Cigarette smoke exposure decreased arterial HSP70 expression and significantly increased intimal thickening compared with mice exposed to air. Treatment of mice exposed to cigarette smoke with intravenous recombinant HSP70 attenuated intimal thickening through reduced phosphorylated extracellular signal-regulated kinase (pERK) expression in the arterial wall. In vitro experiments with rat aortic smooth muscle cells confirmed that recombinant HSP70 decreases pERK and proliferating cell nuclear antigen (PCNA) expression in cells exposed to cigarette smoke extract and H2O2. Our study suggests that decreased expression of arterial HSP70 is an important mechanism by which exposure to cigarette smoke augments intimal thickening. The effects of recombinant HSP70 suggest a role for extracellular HSP70.

scholarly journals THE EFFECT OF PSYCHOLOGICAL STRESS ON MPF INTRAFOLLICULAR

2019 ◽  
pp. 127-130
Author(s):  
REVI GH NOVIKA ◽  
BUDI SANTOSO ◽  
WIDJIATI WIDJIATI
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Psychological Stress ◽  
Heat Shock Protein 70 ◽  
Regulatory Protein ◽  
Control Group ◽  
Hsp70 Expression ◽  
Control Group Design ◽  
Cortisol Levels ◽  
Experimental Group

Objective: The aim of the study is to investigate the influence of cortisol and Heat Shock Protein 70 (HSP70) on the stressed mice to maturationpromoting factor (MPF) expression intrafollicular.Methods: Experimental laboratory with Randomized Post Test Only Control Group Design was carried out on intrafollicular mice. Divided into twogroups, experimental and control groups. The experimental group was given a 95 dB 4 hours/day noisy exposure for 5 days which was analogous topsychological stress in humans and the control group was not given noisy exposure. Furthermore, both groups were examined for cortisol levels toensure stress in mice. Heat shock protein 70 (HSP70) expression was examined as the main regulatory protein for stress response and Maturationpromoting factor (MPF) expression which is a mediator for oocyte maturation.Results: Psychological stress by 95 dB/4 hours/day noisy exposure for 5 days significantly increased serum cortisol levels in experimental group(p=0.000) and HSP70 expression (p=0.000). The effect of Increased cortisol levels and HSP70 expression significantly decrease in MPF expression(p=0.000).Conclusion: The study concluded that psychological stress could be seen by increasing cortisol and HSP70 expression affected to decreasing MPFexpression intrafollicular

Inducing heat shock protein 70 expression provides a robust anti-thrombotic effect with minimal bleeding risk

2017 ◽  
Vol 117 (09) ◽  
pp. 1722-1729 ◽  
Author(s):  
Mikel Allende ◽  
Eva Molina ◽  
Ramón Lecumberri ◽  
Juan Sánchez-Arias ◽  
Ana Ugarte ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Ferric Chloride ◽  
Heat Shock Protein 70 ◽  
Bleeding Risk ◽  
Rank Test ◽  
Control Group ◽  
Hsp70 Expression ◽  
Bleeding Tendency ◽  
Dual Inhibitor

SummaryAntithrombotic medications target coagulation factors. Their use is associated with an increased bleeding risk. Safer drugs are needed. The heat shock protein 70 (Hsp70) exhibits antithrombotic properties that do not influence bleeding. By using murine models, we aimed to test the hypothesis that overexpressing Hsp70 with CM-695, a first in class dual inhibitor of HDAC6 and phosphodiesterase 9, protects against thrombosis while leaves bleeding tendency unaltered. CM-695 was used to induce Hsp70 overexpression. Hsp70 overexpressing mice were submitted to three thrombosis-triggering procedures. The ferric chloride carotid artery model was used to compare the antithrombotic role of CM-695 and rivaroxaban, a direct oral anticoagulant. The mouse tail transection model was used to compare the bleeding tendency upon CM-695 or rivaroxaban administration. Intraperitoneal (i. p.) 20 mg/kg CM-695 increased Hsp70 expression markedly in the murine aortic tissue. This treatment delayed thrombosis in the collagen/epinephrine [p=0.04 (Log-Rank test), n=10], Rose Bengal/laser [median vessel occlusion time (OT): 58.6 vs 39.0 minutes (min) in the control group (CG), p=0.008, n≥10] and ferric chloride (OT: 14.7 vs 9.2 min in the CG, p=0.032, n≥10) models. I.p. 80 mg/kg CM-695 (n≥9) and intravenous 3 mg/kg rivaroxaban (n≥8) significantly delayed thrombosis. CM-695 did not induce bleeding [median bleeding time (BT): 8.5 vs 7.5 min in the CG, n≥10]. However, BT was dramatically increased by rivaroxaban (30.0 vs 13.7 min in the CG, p=0.001, n=10). In conclusion, CM-695 is a new antithrombotic small molecule devoid of bleeding risk that may be envisioned as a useful clinical tool.

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