Origin, localization and binding abilities of boar DQH sperm surface protein tested by specific monoclonal antibodies

2007 ◽  
Vol 74 (1-2) ◽  
pp. 103-113 ◽  
Author(s):  
P. Maňásková ◽  
J. Pěknicová ◽  
F. Elzeinová ◽  
M. Tichá ◽  
V. Jonáková
Keyword(s):  
Monoclonal Antibodies ◽  
Surface Protein ◽  
Sperm Surface

scholarly journals The Ubiquitin-Proteasome System Does Not Regulate the Degradation of Porcine β-Microseminoprotein during Sperm Capacitation

2020 ◽  
Vol 21 (11) ◽  
pp. 4151
Author(s):  
Lucie Tumova ◽  
Michal Zigo ◽  
Peter Sutovsky ◽  
Marketa Sedmikova ◽  
Pavla Postlerova
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Protein Composition ◽  
Surface Protein ◽  
Ubiquitin Proteasome System ◽  
Sperm Capacitation ◽  
Sperm Surface ◽  
Ubiquitin Proteasome ◽  
Seminal Plasma Proteins

Sperm capacitation, one of the key events during successful fertilization, is associated with extensive structural and functional sperm remodeling, beginning with the modification of protein composition within the sperm plasma membrane. The ubiquitin-proteasome system (UPS), a multiprotein complex responsible for protein degradation and turnover, participates in capacitation events. Previous studies showed that capacitation-induced shedding of the seminal plasma proteins such as SPINK2, AQN1, and DQH from the sperm surface is regulated by UPS. Alterations in the sperm surface protein composition also relate to the porcine β-microseminoprotein (MSMB/PSP94), seminal plasma protein known as immunoglobulin-binding factor, and motility inhibitor. MSMB was detected in the acrosomal region as well as the flagellum of ejaculated boar spermatozoa, while the signal disappeared from the acrosomal region after in vitro capacitation (IVC). The involvement of UPS in the MSMB degradation during sperm IVC was studied using proteasomal interference and ubiquitin-activating enzyme (E1) inhibiting conditions by image-based flow cytometry and Western blot detection. Our results showed no accumulation of porcine MSMB either under proteasomal inhibition or under E1 inhibiting conditions. In addition, the immunoprecipitation study did not detect any ubiquitination of sperm MSMB nor was MSMB detected in the affinity-purified fraction containing ubiquitinated sperm proteins. Based on our results, we conclude that UPS does not appear to be the regulatory mechanism in the case of MSMB and opening new questions for further studies. Thus, the capacitation-induced processing of seminal plasma proteins on the sperm surface may be more complex than previously thought, employing multiple proteolytic systems in a non-redundant manner.

scholarly journals A Histoplasma capsulatum-Specific IgG1 Isotype Monoclonal Antibody, H1C, to a 70-Kilodalton Cell Surface Protein Is Not Protective in Murine Histoplasmosis

2010 ◽  
Vol 17 (7) ◽  
pp. 1155-1158 ◽  
Author(s):  
Livia Cristina Liporagi Lopes ◽  
Allan J. Guimarães ◽  
Mariana Duarte de Cerqueira ◽  
Beatriz L. Gómez ◽  
Joshua D. Nosanchuk
Keyword(s):  
Nitric Oxide ◽  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Histoplasma Capsulatum ◽  
Fungal Growth ◽  
Surface Protein ◽  
Cell Surface Protein ◽  
Fungal Burden ◽  
Nitric Oxide Release ◽  
Igg1 Isotype

ABSTRACT Monoclonal antibodies to Histoplasma capsulatum can modify pathogenesis. We now show that monoclonal antibody H1C to a 70-kDa antigen increases intracellular fungal growth and reduces macrophage nitric oxide release but has no effect on fungal burden or survival in murine infection. This further demonstrates the complexities of host-pathogen interactions.

A map of the guinea pig sperm surface constructed with monoclonal antibodies

1983 ◽  
Vol 98 (2) ◽  
pp. 417-428 ◽  
Author(s):  
Paul Primakoff ◽  
Diana Gold Myles
Keyword(s):  
Monoclonal Antibodies ◽  
Guinea Pig ◽  
Sperm Surface

scholarly journals Analysis of the murine sperm surface with monoclonal antibodies

Reproduction ◽  
1983 ◽  
Vol 68 (2) ◽  
pp. 497-505 ◽  
Author(s):  
D. N. Crichton ◽  
B. B. Cohen
Keyword(s):  
Monoclonal Antibodies ◽  
Sperm Surface

Monoclonal antibodies to sperm surface antigens

1983 ◽  
Vol 5 ◽  
pp. 25-26
Author(s):  
Chi-Yu Gregory Lee ◽  
Eric Wong ◽  
Vincent Lum ◽  
Evelyn Low ◽  
Alan C. Menge ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Surface Antigens ◽  
Sperm Surface
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