Quantitative proteomics of psychotrophic diazotroph in response to nitrogen deficiency and cold stress

2018 ◽  
Vol 187 ◽  
pp. 235-242 ◽  
Author(s):  
Deep Chandra Suyal ◽  
Saurabh Kumar ◽  
Divya Joshi ◽  
Ravindra Soni ◽  
Reeta Goel
Keyword(s):  
Cold Stress ◽  
Quantitative Proteomics ◽  
Nitrogen Deficiency

iTRAQ-based quantitative proteomics analysis of cold stress-induced mechanisms in grafted watermelon seedlings

2019 ◽  
Vol 192 ◽  
pp. 311-320 ◽  
Author(s):  
Xianfeng Shi ◽  
Xibo Wang ◽  
Fei Cheng ◽  
Haishun Cao ◽  
Huang Liang ◽  
...  
Keyword(s):  
Cold Stress ◽  
Quantitative Proteomics ◽  
Proteomics Analysis

scholarly journals ITRAQ-based quantitative proteomic analysis of japonica rice seedling during cold stress

2021 ◽  
Author(s):  
Dongjin Qing ◽  
Yinghua Pan ◽  
Gaoxing Dai ◽  
Lijun Gao ◽  
Haifu Liang ◽  
...  
Keyword(s):  
Cold Stress ◽  
Stress Responses ◽  
Protein Level ◽  
Quantitative Proteomics ◽  
Rice Variety ◽  
Mrna Level ◽  
Growth And Yield ◽  
Pcr Analysis ◽  
Control Group ◽  
Japonica Rice

Low temperature is one of the important environmental factors that affect rice growth and yield. To better understand the japonica rice responses to cold stress, isobaric tags for relative and absolute quantification (iTRAQ) labeling based quantitative proteomics approach was used to detected changes in protein level. Two-week-old seedlings of the cold tolerance rice variety Kongyu131 were treated at 8? for 24, 48 and 72 h, then the total proteins were extracted from tissues and used for quantitative proteomics analysis. A total of 5082 proteins were detected for quantitative analysis, of which 289 proteins were significantly regulated, consisting of 169 uniquely up-regulated proteins and 125 uniquely down-regulated proteins in cold stress groups relative to control group. Functional analysis revealed most of regulation proteins involved in photosynthesis, metabolic pathway, biosynthesis of secondary metabolites and carbon metabolism. Western blot analysis showed that protein regulation was consistent with the iTRAQ data. The corresponding genes of 25 regulation proteins were used for quantitative real time PCR analysis, and the results showed that the mRNA level was not always parallel to the corresponding protein level. The importance of our study is providing new insights into cold stress responses in rice on proteomic aspect.

Pathological features of the Florida manatee cold stress syndrome

2003 ◽  
Vol 29 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Gregory D. Bossart ◽  
René A. Meisner ◽  
S. A. Rommel ◽  
Shin-Je Ghim ◽  
A. Bennett Jenson
Keyword(s):  
Cold Stress ◽  
Pathological Features ◽  
Florida Manatee ◽  
Stress Syndrome
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