Secretomes of Mycoplasma hyopneumoniae and Mycoplasma flocculare reveal differences associated to pathogenesis

2017 ◽  
Vol 154 ◽  
pp. 69-77 ◽  
Author(s):  
Jéssica A. Paes ◽  
Karina R. Lorenzatto ◽  
Sofia N. de Moraes ◽  
Hercules Moura ◽  
John R. Barr ◽  
...  
Keyword(s):  
Mycoplasma Hyopneumoniae ◽  
Mycoplasma Flocculare

Mycoplasma hyopneumoniae and Mycoplasma flocculare differential domains from orthologous surface proteins induce distinct cellular immune responses in mice

2016 ◽  
Vol 190 ◽  
pp. 50-57 ◽  
Author(s):  
Fernanda Munhoz dos Anjos Leal ◽  
Veridiana Gomes Virginio ◽  
Carolina Lumertz Martello ◽  
Jéssica Andrade Paes ◽  
Thiago J. Borges ◽  
...  
Keyword(s):  
Immune Responses ◽  
Mycoplasma Hyopneumoniae ◽  
Surface Proteins ◽  
Cellular Immune Responses ◽  
Cellular Immune ◽  
Mycoplasma Flocculare

scholarly journals Mycoplasma hyopneumoniae Increases Intracellular Calcium Release in Porcine Ciliated Tracheal Cells

2002 ◽  
Vol 70 (5) ◽  
pp. 2502-2506 ◽  
Author(s):  
Seung-Chun Park ◽  
Sirintorn Yibchok-Anun ◽  
Henrique Cheng ◽  
Theresa F. Young ◽  
Eileen L. Thacker ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Epithelial Cells ◽  
Phospholipase C ◽  
Pertussis Toxin ◽  
Calcium Release ◽  
Mycoplasma Hyopneumoniae ◽  
Tracheal Epithelial Cells ◽  
Tracheal Epithelial ◽  
Mycoplasma Flocculare ◽  
Ciliated Epithelial Cells

ABSTRACT We investigated the effects of intact pathogenic Mycoplasma hyopneumoniae, nonpathogenic M. hyopneumoniae, and Mycoplasma flocculare on intracellular free Ca2+ concentrations ([Ca2+]i) in porcine ciliated tracheal epithelial cells. The ciliated epithelial cells had basal [Ca2+]i of 103 ± 3 nM (n = 217 cells). The [Ca2+]i increased by 250 ± 19 nM (n = 47 cells) from the basal level within 100 s of the addition of pathogenic M. hyopneumoniae strain 91-3 (300 μg/ml), and this increase lasted ∼60 s. In contrast, nonpathogenic M. hyopneumoniae and M. flocculare at concentrations of 300 μg/ml failed to increase [Ca2+]i. In Ca2+-free medium, pathogenic M. hyopneumoniae still increased [Ca2+]i in tracheal cells. Pretreatment with thapsigargin (1 μM for 30 min), which depleted the Ca2+ store in the endoplasmic reticulum, abolished the effect of M. hyoneumoniae. Pretreatment with pertussis toxin (100 ng/ml for 3 h) or U-73122 (2 μM for 100 s), an inhibitor of phospholipase C, also abolished the effect of M. hyopneumoniae. The administration of mastoparan 7, an activator of pertussis toxin-sensitive proteins Gi and Go, increased [Ca2+]i in ciliated tracheal cells. These results suggest that pathogenic M. hyopneumoniae activates receptors that are coupled to Gi or Go, which in turn activates a phospholipase C pathway, thereby releasing Ca2+ from the endoplasmic reticulum. Thus, an increase in Ca2+ may serve as a signal for the pathogenesis of M. hyopneumoniae.

Evidences of differential endoproteolytic processing on the surfaces of Mycoplasma hyopneumoniae and Mycoplasma flocculare

2020 ◽  
Vol 140 ◽  
pp. 103958 ◽  
Author(s):  
Lais Del Prá Netto Machado ◽  
Jéssica Andrade Paes ◽  
Priscila Souza dos Santos ◽  
Henrique Bunselmeyer Ferreira
Keyword(s):  
Mycoplasma Hyopneumoniae ◽  
Mycoplasma Flocculare

Co-infections by Mycoplasma hyopneumoniae, Mycoplasma hyorhinis and Mycoplasma flocculare in macroscopic lesions of lung consolidation of pigs at slaughter

2021 ◽  
pp. 109123
Author(s):  
Marcela Manduca Ferreira ◽  
Marina Lopes Mechler Dreibi ◽  
Karina Sonalio ◽  
Henrique Meiroz de Souza Almeida ◽  
Maria Eugênia Silveira Ferraz ◽  
...  
Keyword(s):  
Mycoplasma Hyopneumoniae ◽  
Mycoplasma Hyorhinis ◽  
Lung Consolidation ◽  
Mycoplasma Flocculare

scholarly journals Padronização de três ELISAs polivalentes com lipopolissacarídeos de cadeia longa dos sorotipos 1 e 5, 2, 3 e 7 ou 10 e 12 de Actinobacillus pleuropneumoniae

2008 ◽  
Vol 60 (2) ◽  
pp. 377-383
Author(s):  
S.S. Kuchiishi ◽  
L.F.O.S. Carvalho ◽  
I.A. Piffer ◽  
J.D. Kich ◽  
M.L.F. Ramenzoni
Keyword(s):  
Mycoplasma Hyopneumoniae ◽  
Actinobacillus Pleuropneumoniae ◽  
Haemophilus Parasuis ◽  
Mycoplasma Flocculare

Três ELISAs polivalentes baseados em lipopolissacarídeos de cadeia longa (LPS-CL) foram estabelecidos para detectar anticorpos para todos os sorotipos prevalentes de Actinobacillus pleuropneumoniae. Foram testadas amostras provenientes do banco de soros de suínos experimentalmente inoculados com todos os sorotipos de A. pleuropneumoniae. Os ELISAs foram sensíveis à detecção de anticorpos contra todos os LPS-CL. Foram observadas reações cruzadas no ELISA polivalente produzido com os sorotipos 1 e 5, com anti-soros específicos para os sorotipos 9 e 11, pois os sorotipos 1, 9 e 11 apresentaram antígenos somáticos comuns. No polivalente com os sorotipos 2, 3 e 7, observaram-se reações com anti-soros dos sorotipos 4, 6 e 8, devido à presença de antígenos somáticos entre os sorotipos 3, 6 e 8 e entre os sorotipos 4 e 7. Amostras de soros de animais infectados com Mycoplasma hyopneumoniae, Mycoplasma flocculare e Haemophilus parasuis, agentes que acometem o sistema respiratório dos suínos, não apresentaram reações cruzadas com os antígenos baseados em LPS-CL.

Sign in / Sign up

Export Citation Format

Share Document