Determination of the interactome of non-structural protein12 from highly pathogenic porcine reproductive and respiratory syndrome virus with host cellular proteins using high throughput proteomics and identification of HSP70 as a cellular factor for virus replication

2016 ◽  
Vol 146 ◽  
pp. 58-69 ◽  
Author(s):  
Shan Dong ◽  
Long Liu ◽  
Weining Wu ◽  
Stuart D. Armstrong ◽  
Dong Xia ◽  
...  
Keyword(s):  
Virus Replication ◽  
High Throughput ◽  
Cellular Proteins ◽  
Respiratory Syndrome Virus ◽  
Highly Pathogenic ◽  
Cellular Factor

scholarly journals Identification of Virulence Associated Region during Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus during Attenuation In Vitro: Complex Question with Different Strain Backgrounds

Viruses ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 40
Author(s):  
Yifeng Jiang ◽  
Wu Tong ◽  
Lingxue Yu ◽  
Liwei Li ◽  
Fei Gao ◽  
...  
Keyword(s):  
Virus Replication ◽  
Cytokine Response ◽  
Lung Damage ◽  
Respiratory Syndrome Virus ◽  
Cdna Clones ◽  
Highly Pathogenic ◽  
Prrsv Strain ◽  
Chimeric Viruses

Highly pathogenic porcine reproductive and respiratory syndrome virus PRRSV (HP-PRRSV) was one of the most devastating diseases of the pig industry, among various strategies, vaccination was one of the most useful tools for PRRS control. Attenuated live vaccine was used worldwide, however, the genetic basis of HP-PRRSV virulence change during attenuation remain to be determined. Here, to identify virulence associated regions of HP-PRRSV during attenuation in vitro, six full-length infectious cDNA clones with interchanges of 5′UTR + ORF1a, ORF1b, and ORF2-7 + 3′UTR regions between HP-PRRSV strain HuN4-F5 and its attenuated vaccine strain HuN4-F112 were generated, and chimeric viruses were rescued. Piglets were inoculated with chimeric viruses and their parental viruses, and rectal temperature were recorded daily, and serum were collected for future experiments. Our results showed that ORF1a played an important role on virus replication, cytokine response and lung damage, the exchange of ORF1b and ORF2-7 in different backbone led to different exhibition on virus replication in vivo/vitro and cytokine response. Among 9 PRRSV attenuated series, consistent amino acid changes during PRRSV attenuation were found in NSP4, NSP9, GP2, E, GP3 and GP4. Our study provides a fundamental data for the investigation of PRRSV attenuation, the different results of the virulence change among different studies indicated that different mechanisms might be used during PRRSV virulence enhancement in vivo and attenuation in vitro.

High-throughput determination of flavanone-O-glycosides in citrus beverages by paper spray tandem mass spectrometry

2021 ◽  
pp. 130060
Author(s):  
Fabio Mazzotti ◽  
Lucia Bartella ◽  
Ines Rosita Talarico ◽  
Anna Napoli ◽  
Leonardo Di Donna
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
High Throughput ◽  
Tandem Mass ◽  
Paper Spray
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