Identification of proteins in susceptible and resistant Brassica oleracea responsive to Xanthomonas campestris pv. campestris infection

2016 ◽  
Vol 143 ◽  
pp. 278-285 ◽  
Author(s):  
Gabriela R.C. Villeth ◽  
Lílian S.T. Carmo ◽  
Luciano Paulino Silva ◽  
Mateus Figueiredo Santos ◽  
Osmundo Brilhante de Oliveira Neto ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Xanthomonas Campestris ◽  
Xanthomonas Campestris Pv Campestris

scholarly journals Molecular marking in breeding Brassica oleracea L. for resistance to Xanthomonas campestris pv. campestris

2021 ◽  
Vol 285 ◽  
pp. 03009
Author(s):  
Yuliya Makukha ◽  
Elena Dubina
Keyword(s):  
Brassica Oleracea ◽  
Ssr Markers ◽  
Microsatellite Loci ◽  
Xanthomonas Campestris ◽  
Black Rot ◽  
White Cabbage ◽  
Allelic State ◽  
Xanthomonas Campestris Pv Campestris ◽  
Segregating Population ◽  
Brassica Oleracea L

In the presented study, we have studied polymorphism of 30 microsatellite loci in terms of resistance to black rot (Xanthomonas campestris). Three SSR markers (Ol10-C01, Ol11-H06, BoESSR 726) were identified, showing polymorphism in white cabbage forms with contrasting resistance to black rot. Also, part of the F2 breeding material was analyzed using markers Ol10-C01, Ol11-H06. It was found that they reveal all types of allelic state of genes in a segregating population in accordance with the Mendelian rule of segregation, which makes these markers suitable for further use in studies on the basis of cabbage resistance to black rot.

Development of a marker for detection of Xanthomonas campestris pv. campestris races 1 and 2 in Brassica oleracea

2019 ◽  
Vol 60 (4) ◽  
pp. 511-517
Author(s):  
Mehede Hassan Rubel ◽  
Sathishkumar Natarajan ◽  
Ujjal Kumar Nath ◽  
Michael Immanuel Jesse Denison ◽  
Jung Hee-Jeong ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Xanthomonas Campestris ◽  
Xanthomonas Campestris Pv Campestris

scholarly journals Detecção, transmissão e efeito de Xanthomonas campestris pv. campestris na qualidade fisiológica de sementes de brócolis

2007 ◽  
Vol 33 (4) ◽  
pp. 416-418 ◽  
Author(s):  
Nilvanira Donizeti Tebaldi ◽  
Rita de Cássia Panizzi ◽  
Rubens Sader
Keyword(s):  
Brassica Oleracea ◽  
Xanthomonas Campestris ◽  
Xanthomonas Campestris Pv Campestris

A detecção, a transmissão e o efeito de Xanthomonas campestris pv. campestris (Xcc) na qualidade fisiológica de sementes de brócolis (Brassica oleracea var. italica) foram avaliados, a partir de sementes obtidas de plantas ("Baron, Flórida, Hana Midori Sakata, Precoce Piracicaba de Verão, Ramoso Santana e Sabre") inoculadas com a bactéria, em condições de campo. Para a detecção do patógeno nas sementes foram utilizados os meios de cultura semi-seletivos: SX ágar, NSCAA e BSCAA; a taxa de transmissão da bactéria pelas sementes às plântulas foi avaliada usando semeadura em areia e meio de cultura contido em tubo de ensaio. Para a avaliação da qualidade fisiológica de sementes foram realizados o teste padrão de germinação e os testes de vigor: envelhecimento acelerado, índice de velocidade de emergência, crescimento de plântulas e massa seca. De acordo com os resultados, o meio de cultura semi-seletivo NSCAA foi mais eficaz para detectar Xcc em sementes de brócolis; não houve diferença significativa entre os genótipos na taxa de transmissão da bactéria pelas sementes e Xcc não afetou a germinação e o vigor das sementes.

scholarly journals Occurrence of Xanthomonas campestris pv. campestris (Pammel, 1895) Dowson 1939, on brassicas in Montenegro

2012 ◽  
Vol 27 (2) ◽  
pp. 131-140 ◽  
Author(s):  
Dragana Radunovic ◽  
Jelica Balaz
Keyword(s):  
Brassica Oleracea ◽  
Xanthomonas Campestris ◽  
Vascular Tissue ◽  
Elisa Test ◽  
Vegetable Production ◽  
Vegetable Crops ◽  
Isolation And Identification ◽  
Laboratory Methods ◽  
Xanthomonas Campestris Pv Campestris ◽  
Successful Production

Brassicas form the most important group of vegetable crops in Montenegro. The cabbage (Brassica oleracea var. capitata) is most commonly grown, although other brassicas, particularly kale, Brussels sprout, cauliflower and broccoli, have been increasingly produced since recently. One of the specialties of vegetable production in Montenegro is growing of collard (Brassica oleracea var. acephala), which is the simplest variety of the Brassica oleracea species and in the nearest relation with their wild ancestor - the sylvestris variety. Diseases are the main restrictive factors for successful production of these vegetables. Susceptibility of the cultivars and inadequate control often result in more or less damaged crops in some plots. Causal agents of brassica diseases, especially bacterial, have not been investigated in Montenegro until 2009. Since the symptoms observed in 2009 were ?V? shaped leaf edge necrosis and black rot of vascular tissue, it was assumed that they were caused by plant pathogenic bacterium Xanthomonas campestris pv. campestris. Samples of the infected plants were collected from different localities in Montenegro. Isolation and identification of the bacterium were performed using laboratory methods according to Schaad (1980), Lelliott and Stead (1987) and Arsenijevic (1997). Examination of chosen bacterial isolates was conducted using both, classical bacteriological methods (examination of their pathogenic, morphological, cultivation and biochemical and physiological characteristics), and ELISA test. The obtained results confirmed the presence of X.campestris pv. campestris (Pammel, 1895) Dowson 1939, on cabbage, kale, broccoli and collard in Montenegro. This is the first experimental evidence that collard is the host of X. campestris pv. campestris in Montenegro.

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