scholarly journals Candida albicans cell shaving uncovers new proteins involved in cell wall integrity, yeast to hypha transition, stress response and host–pathogen interaction

2015 ◽  
Vol 127 ◽  
pp. 340-351 ◽  
Author(s):  
Ana Gil-Bona ◽  
Claudia Marcela Parra-Giraldo ◽  
María Luisa Hernáez ◽  
Jose Antonio Reales-Calderon ◽  
Norma V. Solis ◽  
...  
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Stress Response ◽  
Cell Wall Integrity ◽  
Transition Stress ◽  
Host Pathogen Interaction ◽  
Host Pathogen

scholarly journals Proteomic Analysis of Caspofungin-Induced Responses in Planktonic Cells and Biofilms of Candida albicans

2021 ◽  
Vol 12 ◽  
Author(s):  
Peng Li ◽  
Chaminda J. Seneviratne ◽  
Qingxian Luan ◽  
Lijian Jin
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Stress Response ◽  
Laser Scanning ◽  
Metabolic Regulation ◽  
Antifungal Resistance ◽  
Laser Scanning Microscopy ◽  
Cell Wall Integrity ◽  
Confocal Laser ◽  
Planktonic Cells

Candida albicans biofilms display markedly increased antifungal resistance, and the underlying mechanisms remain unclear. This study investigated the signature profiles of C. albicans planktonic cells and biofilms in response to caspofungin (CAS) by mass spectrometry-based shotgun proteomics. We found that C. albicans biofilms were twofold more resistant to CAS with reference to planktonic cells. Notably, 9.6% of C. albicans biofilm cells survived the lethal treatment of CAS (128 μg/ml), confirmed by LIVE/DEAD staining, confocal laser scanning microscopy (CLSM) and scanning electron microscopy analyses. The responses of C. albicans planktonic cells and biofilms to CAS treatment at respective minimum inhibitory concentrations (MICs) were assessed by high-throughput proteomics and bioinformatics approaches. There were 148 and 224 proteins with >twofold difference identified from the planktonic cells and biofilms, respectively. CAS treatment downregulated several cell wall- and oxidative stress-related proteins. Whereas, CAS-induced action was compensated by markedly increased expression of many other proteins involved in cell wall integrity and stress response (e.g., heat shock proteins). Moreover, considerable expression changes were identified in metabolism-associated proteins like glycolysis, tricarboxylic acid (TCA) cycle and ATP biosynthesis. Importantly, various key proteins for cell wall integrity, stress response and metabolic regulation (e.g., PIL1, LSP1, HSP90, ICL1, and MLS1) were exclusively enriched and implicated in C. albicans biofilms. This study demonstrates that C. albicans biofilms undergo highly complicated yet complex regulation of multiple cellular pathways in response to CAS. Signature proteins essential for modulating cell wall integrity, stress response and metabolic activities may account for the antifungal resistance of C. albicans biofilms.

scholarly journals UDP-glucose 4, 6-dehydratase Activity Plays an Important Role in Maintaining Cell Wall Integrity and Virulence of Candida albicans

2011 ◽  
Vol 7 (11) ◽  
pp. e1002384 ◽  
Author(s):  
Manimala Sen ◽  
Bhavin Shah ◽  
Srabanti Rakshit ◽  
Vijender Singh ◽  
Bhavna Padmanabhan ◽  
...  
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Cell Wall Integrity ◽  
Dehydratase Activity

scholarly journals A novel connection between the Cell Wall Integrity and the PKA pathways regulates cell wall stress response in yeast

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Raúl García ◽  
Enrique Bravo ◽  
Sonia Diez-Muñiz ◽  
Cesar Nombela ◽  
Jose M. Rodríguez-Peña ◽  
...  
Keyword(s):  
Cell Wall ◽  
Stress Response ◽  
Wall Stress ◽  
Cell Wall Integrity ◽  
Cell Wall Stress

Deletion of the Candida albicans PIR32 Results in Increased Virulence, Stress Response, and Upregulation of Cell Wall Chitin Deposition

2012 ◽  
Vol 174 (2) ◽  
pp. 107-119 ◽  
Author(s):  
Wael Bahnan ◽  
Joseph Koussa ◽  
Samer Younes ◽  
Marybel Abi Rizk ◽  
Bassem Khalil ◽  
...  
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Stress Response

scholarly journals KRE5 Suppression Induces Cell Wall Stress and Alternative ER Stress Response Required for Maintaining Cell Wall Integrity in Candida glabrata

PLoS ONE ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. e0161371 ◽  
Author(s):  
Yutaka Tanaka ◽  
Masato Sasaki ◽  
Fumie Ito ◽  
Toshio Aoyama ◽  
Michiyo Sato-Okamoto ◽  
...  
Keyword(s):  
Cell Wall ◽  
Stress Response ◽  
Er Stress ◽  
Candida Glabrata ◽  
Wall Stress ◽  
Cell Wall Integrity ◽  
Er Stress Response ◽  
Cell Wall Stress

scholarly journals Critical role for CaFEN1 and CaFEN12 of Candida albicans in cell wall integrity and biofilm formation

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Md. Alfatah ◽  
Vinay K. Bari ◽  
Anubhav S. Nahar ◽  
Swati Bijlani ◽  
K. Ganesan
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Biofilm Formation ◽  
Critical Role ◽  
Cell Wall Integrity
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