Phosphoproteome analyses reveal specific implications of Hcls1, p21-activated kinase 1 and Ezrin in proliferation of a myeloid progenitor cell line downstream of wild-type and ITD mutant Fms-like tyrosine kinase 3 receptors

2013 ◽  
Vol 78 ◽  
pp. 231-244 ◽  
Author(s):  
Guillaume Habif ◽  
Marie-France Grasset ◽  
Sylvie Kieffer-Jaquinod ◽  
Lauriane Kuhn ◽  
Guy Mouchiroud ◽  
...  
Keyword(s):  
Tyrosine Kinase ◽  
Cell Line ◽  
Progenitor Cell ◽  
Wild Type ◽  
Myeloid Progenitor ◽  
Myeloid Progenitor Cell ◽  
P21 Activated Kinase ◽  
Progenitor Cell Line

Two New Pseudopod Morphologies Displayed by the Human Hematopoietic KG1a Progenitor Cell Line and by Primary Human CD34+Cells

Blood ◽  
1998 ◽  
Vol 92 (10) ◽  
pp. 3616-3623 ◽  
Author(s):  
Karl Francis ◽  
Ramprasad Ramakrishna ◽  
William Holloway ◽  
Bernhard O. Palsson
Keyword(s):  
Bone Marrow ◽  
Cell Line ◽  
Progenitor Cell ◽  
Fetal Liver ◽  
Human Cd34 ◽  
Myeloid Progenitor ◽  
Cd34 Cells ◽  
Cd44 Antigens ◽  
Adult Bone ◽  
Progenitor Cell Line

Abstract A primitive human hematopoietic myeloid progenitor cell line, KG1a, characterized by high expression of the CD34 surface antigen has been observed to extend long, thin pseudopodia. Once extended, these pseudopods may take on one of two newly described morphologies, tenupodia or magnupodia. Tenupodia are very thin and form in linear segments. They adhere to the substrate, can bifurcate multiple times, and often appear to connect the membranes of cells more than 300 μm apart. Magnupodia are much thicker and have been observed to extend more than 330 μm away from the cell. Magnupods are flexible and can exhibit rapid dynamic motion, extending or retracting in a few seconds. During retraction, the extended material often pools into a bulb located on the pod. Both morphologies can adhere to substrates coated with fibronectin, collagen IV, and laminin as well as plastic. The CD34 and CD44 antigens are also present on the surface of these podia. Primary human CD34+ cells from fetal liver, umbilical cord blood, adult bone marrow, and mobilized peripheral blood extend these podia as well. The morphology that these pseudopods exhibit suggest that they may play both sensory and mechanical roles during cell migration and homing after bone marrow transplantation.

Two New Pseudopod Morphologies Displayed by the Human Hematopoietic KG1a Progenitor Cell Line and by Primary Human CD34+Cells

Blood ◽  
1998 ◽  
Vol 92 (10) ◽  
pp. 3616-3623 ◽  
Author(s):  
Karl Francis ◽  
Ramprasad Ramakrishna ◽  
William Holloway ◽  
Bernhard O. Palsson
Keyword(s):  
Bone Marrow ◽  
Cell Line ◽  
Progenitor Cell ◽  
Fetal Liver ◽  
Human Cd34 ◽  
Myeloid Progenitor ◽  
Cd34 Cells ◽  
Cd44 Antigens ◽  
Adult Bone ◽  
Progenitor Cell Line

A primitive human hematopoietic myeloid progenitor cell line, KG1a, characterized by high expression of the CD34 surface antigen has been observed to extend long, thin pseudopodia. Once extended, these pseudopods may take on one of two newly described morphologies, tenupodia or magnupodia. Tenupodia are very thin and form in linear segments. They adhere to the substrate, can bifurcate multiple times, and often appear to connect the membranes of cells more than 300 μm apart. Magnupodia are much thicker and have been observed to extend more than 330 μm away from the cell. Magnupods are flexible and can exhibit rapid dynamic motion, extending or retracting in a few seconds. During retraction, the extended material often pools into a bulb located on the pod. Both morphologies can adhere to substrates coated with fibronectin, collagen IV, and laminin as well as plastic. The CD34 and CD44 antigens are also present on the surface of these podia. Primary human CD34+ cells from fetal liver, umbilical cord blood, adult bone marrow, and mobilized peripheral blood extend these podia as well. The morphology that these pseudopods exhibit suggest that they may play both sensory and mechanical roles during cell migration and homing after bone marrow transplantation.

scholarly journals Activation of apoptosis associated with enforced myc expression in myeloid progenitor cells is dominant to the suppression of apoptosis by interleukin-3 or erythropoietin

Blood ◽  
1993 ◽  
Vol 82 (7) ◽  
pp. 2079-2087 ◽  
Author(s):  
DS Askew ◽  
JN Ihle ◽  
JL Cleveland
Keyword(s):  
Progenitor Cell ◽  
G1 Arrest ◽  
Interleukin 3 ◽  
Cycle Arrest ◽  
Myeloid Progenitor ◽  
Myeloid Progenitor Cell ◽  
Myeloid Progenitor Cells ◽  
Cell Densities ◽  
Inappropriate Expression ◽  
Progenitor Cell Line

Abstract The inappropriate expression of c-myc in cells deprived of growth factors has recently been implicated in the activation of programmed cell death (apoptosis). The studies described here examine the ability of interleukin-3 (IL-3) or erythropoietin (Epo) to suppress apoptosis that occurs in association with enforced myc expression during cell cycle arrest of a murine IL-3-dependent myeloid progenitor cell line, 32D. G1 arrest was observed when culturing 32D cells to high density in medium supplemented with IL-3, or at subconfluent densities in medium supplemented with Epo. Under both conditions, endogenous c-myc expression was downregulated and viability was maintained. In clones of cells in which c-myc is constitutively expressed from a retroviral vector, enforced c-myc expression was associated with the activation of apoptosis at high cell densities. Similarly, enforced c-myc expression was deleterious to cell survival when these cells were cultured in Epo, as apoptosis was evident within 6 hours. The results support the concept that inappropriate c-myc expression activates apoptosis and that neither IL-3 nor Epo can suppress this program under these conditions.

scholarly journals Activation of apoptosis associated with enforced myc expression in myeloid progenitor cells is dominant to the suppression of apoptosis by interleukin-3 or erythropoietin

Blood ◽  
1993 ◽  
Vol 82 (7) ◽  
pp. 2079-2087 ◽  
Author(s):  
DS Askew ◽  
JN Ihle ◽  
JL Cleveland
Keyword(s):  
Progenitor Cell ◽  
G1 Arrest ◽  
Interleukin 3 ◽  
Cycle Arrest ◽  
Myeloid Progenitor ◽  
Myeloid Progenitor Cell ◽  
Myeloid Progenitor Cells ◽  
Cell Densities ◽  
Inappropriate Expression ◽  
Progenitor Cell Line

The inappropriate expression of c-myc in cells deprived of growth factors has recently been implicated in the activation of programmed cell death (apoptosis). The studies described here examine the ability of interleukin-3 (IL-3) or erythropoietin (Epo) to suppress apoptosis that occurs in association with enforced myc expression during cell cycle arrest of a murine IL-3-dependent myeloid progenitor cell line, 32D. G1 arrest was observed when culturing 32D cells to high density in medium supplemented with IL-3, or at subconfluent densities in medium supplemented with Epo. Under both conditions, endogenous c-myc expression was downregulated and viability was maintained. In clones of cells in which c-myc is constitutively expressed from a retroviral vector, enforced c-myc expression was associated with the activation of apoptosis at high cell densities. Similarly, enforced c-myc expression was deleterious to cell survival when these cells were cultured in Epo, as apoptosis was evident within 6 hours. The results support the concept that inappropriate c-myc expression activates apoptosis and that neither IL-3 nor Epo can suppress this program under these conditions.

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