Identification of five reptile egg whites protein using MALDI-TOF mass spectrometry and LC/MS-MS analysis

2012 ◽  
Vol 75 (6) ◽  
pp. 1940-1959 ◽  
Author(s):  
Bung-on Prajanban ◽  
Laoo Shawsuan ◽  
Sakda Daduang ◽  
Jintana Kommanee ◽  
Sittiruk Roytrakul ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Reptile Egg

scholarly journals Work Flow Analysis of Around-the-clock Processing of Blood Culture Samples and Integrated MALDI-TOF Mass Spectrometry Analysis for the Diagnosis of Bloodstream Infections

2013 ◽  
Vol 59 (11) ◽  
pp. 1649-1656 ◽  
Author(s):  
Wilhelm Schneiderhan ◽  
Alexander Grundt ◽  
Stefan Wörner ◽  
Peter Findeisen ◽  
Michael Neumaier
Keyword(s):  
Mass Spectrometry ◽  
Blood Culture ◽  
Real Time ◽  
Work Flow ◽  
Pathogen Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

BACKGROUND Because sepsis has a high mortality rate, rapid microbiological diagnosis is required to enable efficient therapy. The effectiveness of MALDI-TOF mass spectrometry (MALDI-TOF MS) analysis in reducing turnaround times (TATs) for blood culture (BC) pathogen identification when available in a 24-h hospital setting has not been determined. METHODS On the basis of data from a total number of 912 positive BCs collected within 140 consecutive days and work flow analyses of laboratory diagnostics, we evaluated different models to assess the TATs for batch-wise and for immediate response (real-time) MALDI-TOF MS pathogen identification of positive BC results during the night shifts. The results were compared to TATs from routine BC processing and biochemical identification performed during regular working hours. RESULTS Continuous BC incubation together with batch-wise MALDI-TOF MS analysis enabled significant reductions of up to 58.7 h in the mean TATs for the reporting of the bacterial species. The TAT of batch-wise MALDI-TOF MS analysis was inferior by a mean of 4.9 h when compared to the model of the immediate work flow under ideal conditions with no constraints in staff availability. CONCLUSIONS Together with continuous cultivation of BC, the 24-h availability of MALDI-TOF MS can reduce the TAT for microbial pathogen identification within a routine clinical laboratory setting. Batch-wise testing of positive BC loses a few hours compared to real-time identification but is still far superior to classical BC processing. Larger prospective studies are required to evaluate the contribution of rapid around-the-clock pathogen identification to medical decision-making for septicemic patients.

scholarly journals Klebsiella MALDI TypeR: a web-based tool for Klebsiella identification based on MALDI-TOF mass spectrometry

2021 ◽  
pp. 103835
Author(s):  
Sébastien Bridel ◽  
Stephen C. Watts ◽  
Louise M. Judd ◽  
Taylor Harshegyi ◽  
Virginie Passet ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Web Based ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

Partial Fluorination of Hydroxyl End-Capped Oligoesters Revealed by MALDI−TOF Mass Spectrometry

2001 ◽  
Vol 34 (7) ◽  
pp. 2389-2393 ◽  
Author(s):  
Weihua Ming ◽  
Xianwen Lou ◽  
Robert D. van de Grampel ◽  
Joost L. J. van Dongen ◽  
Rob van der Linde
Keyword(s):  
Mass Spectrometry ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

Catheter-related Mycobacterium fortuitum Bloodstream Infection: Rapid Identification Using MALDI-TOF Mass Spectrometry

2014 ◽  
Vol 226 (02) ◽  
pp. 68-71 ◽  
Author(s):  
M. Artacho-Reinoso ◽  
P. Olbrich ◽  
P. Solano-Paéz ◽  
P. Ybot-Gonzalez ◽  
J. Lepe ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Bloodstream Infection ◽  
Rapid Identification ◽  
Mycobacterium Fortuitum ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

scholarly journals Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry

2016 ◽  
Vol 120 ◽  
pp. 68-71 ◽  
Author(s):  
Gerald Larrouy-Maumus ◽  
Abigail Clements ◽  
Alain Filloux ◽  
Ronan R. McCarthy ◽  
Serge Mostowy
Keyword(s):  
Mass Spectrometry ◽  
Lipid A ◽  
Direct Detection ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

MiS-MALDI: microgel-selected detection of protein biomarkers by MALDI-ToF mass spectrometry

2010 ◽  
Vol 6 (11) ◽  
pp. 2214 ◽  
Author(s):  
Eleonora Cerasoli ◽  
Paulina D. Rakowska ◽  
Adrian Horgan ◽  
Jascindra Ravi ◽  
Melanie Bradley ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Biomarkers ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof
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