Application of subproteomics in the characterization of Gram-positive bacteria

2012 ◽  
Vol 75 (10) ◽  
pp. 2803-2810 ◽  
Author(s):  
Xiao-Yan Yang ◽  
Jie Lu ◽  
Xuesong Sun ◽  
Qing-Yu He
Keyword(s):  
Gram Positive ◽  
Gram Positive Bacteria

scholarly journals Crystal structures and characterization of different thermostable Penicillin G acylases (PGAs) from Gram-positive bacteria

2018 ◽  
Vol 90 (9) ◽  
pp. 1277-1277
Author(s):  
J. Mayer ◽  
G. Günther ◽  
J. Pippel ◽  
A. Lauermann ◽  
M. Kubiak ◽  
...  
Keyword(s):  
Crystal Structures ◽  
Penicillin G ◽  
Gram Positive ◽  
Gram Positive Bacteria

scholarly journals Characterization of unusual tetracycline-resistant gram-positive bacteria.

1991 ◽  
Vol 35 (12) ◽  
pp. 2655-2657 ◽  
Author(s):  
M C Roberts ◽  
B J Moncla ◽  
S L Hillier
Keyword(s):  
Gram Positive ◽  
Gram Positive Bacteria

scholarly journals Molecular Characterization of Zinc (Zn) Resistant Bacteria in Banger River, Pekalongan, Indonesia

2018 ◽  
Vol 10 (3) ◽  
pp. 622-628
Author(s):  
Fitri Arum Sasi ◽  
Hermin Pancasakti Kusumaningrum ◽  
Anto Budiharjo
Keyword(s):  
Bacterial Species ◽  
Selective Medium ◽  
Resistant Bacteria ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Indigenous Bacteria ◽  
Gram Positive Bacteria ◽  
Sequences Analysis

Indigenous bacteria are able to remove the metals contamination in environment. This study aimed to assess the resistance of bacterial species to Zinc (Zn) in Banger River, Pekalongan City. The bacteria from three different parts of Banger River were isolated and inoculated in Zn-selective medium. Then, molecular identification to determine the bacteria species was conducted using polymerase chain reaction (PCR) by applying forward-reverse 16SrRNA gene primers. The sequences analysis was conducted using MUSCLE and MEGA6. There were seven dominant species that possibly resistant to Zn. Approximately, every isolate could reach more than 95 % from 2000 ppm of Zn in the medium. The higher absorption of Zn was found in Z5 isolate. The seven bacteria species were clustered into nine genera i.e. Klebsiela, Xenorhabdus, Cronobacter, Enterobacter, Escherichia, Shigella and Sporomusa known as Gram Negative bacteria and Clostridium and Bacillus as Gram Positive bacteria. In Gram Positive bacteria, especially Bacillus sp, carboxyl group in peptidoglycan play a role as metal binder. In Gram-negative bacteria, lipopolysaccharide (LPS) which is highly anionic component on the outer membrane, able to catch the Zn. Besides that, Enterobacter activates endogen antioxidants such as glutathione peroxidase (GSHPx), glutathione reductase (GR), catalase (CAT) and superoxide dismutase (SOD). The research found there was possible seven novel indigenous bacteria species in Banger that able to remove Zn from the sediment extremely. This finding can be developed as an eco-friendly approach to reduce metals pollution using local microorganisms.

Developments in the use ofBacillusspecies for industrial production

2004 ◽  
Vol 50 (1) ◽  
pp. 1-17 ◽  
Author(s):  
Marcus Schallmey ◽  
Ajay Singh ◽  
Owen P Ward
Keyword(s):  
Extracellular Enzymes ◽  
Protein Translocation ◽  
Bacillus Species ◽  
Heterologous Proteins ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Complex Protein ◽  
High Yields ◽  
Bacillus Strains

Bacillus species continue to be dominant bacterial workhorses in microbial fermentations. Bacillus subtilis (natto) is the key microbial participant in the ongoing production of the soya-based traditional natto fermentation, and some Bacillus species are on the Food and Drug Administration's GRAS (generally regarded as safe) list. The capacity of selected Bacillus strains to produce and secrete large quantities (20–25 g/L) of extracellular enzymes has placed them among the most important industrial enzyme producers. The ability of different species to ferment in the acid, neutral, and alkaline pH ranges, combined with the presence of thermophiles in the genus, has lead to the development of a variety of new commercial enzyme products with the desired temperature, pH activity, and stability properties to address a variety of specific applications. Classical mutation and (or) selection techniques, together with advanced cloning and protein engineering strategies, have been exploited to develop these products. Efforts to produce and secrete high yields of foreign recombinant proteins in Bacillus hosts initially appeared to be hampered by the degradation of the products by the host proteases. Recent studies have revealed that the slow folding of heterologous proteins at the membrane – cell wall interface of Gram-positive bacteria renders them vulnerable to attack by wall-associated proteases. In addition, the presence of thiol-disulphide oxidoreductases in B. subtilis may be beneficial in the secretion of disulphide-bond-containing proteins. Such developments from our understanding of the complex protein translocation machinery of Gram-positive bacteria should allow the resolution of current secretion challenges and make Bacillus species preeminent hosts for heterologous protein production. Bacillus strains have also been developed and engineered as industrial producers of nucleotides, the vitamin riboflavin, the flavor agent ribose, and the supplement poly-γ-glutamic acid. With the recent characterization of the genome of B. subtilis 168 and of some related strains, Bacillus species are poised to become the preferred hosts for the production of many new and improved products as we move through the genomic and proteomic era.Key words: Bacillus, fermentation, enzymes, insecticides, vitamins, antibiotics, D-ribose.

Gram-positive bacteria produce membrane vesicles: Proteomics-based characterization of Staphylococcus aureus-derived membrane vesicles

PROTEOMICS ◽  
2009 ◽  
Vol 9 (24) ◽  
pp. 5425-5436 ◽  
Author(s):  
Eun-Young Lee ◽  
Do-Young Choi ◽  
Dae-Kyum Kim ◽  
Jung-Wook Kim ◽  
Jung Ok Park ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Membrane Vesicles ◽  
Gram Positive ◽  
Gram Positive Bacteria
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