Identification of in vivo protein phosphorylation sites in human pathogen Schistosoma japonicum by a phosphoproteomic approach

2012 ◽  
Vol 75 (3) ◽  
pp. 868-877 ◽  
Author(s):  
Rong Luo ◽  
Chunjing Zhou ◽  
Jiaojiao Lin ◽  
Dehao Yang ◽  
Yaojun Shi ◽  
...  
Keyword(s):  
Protein Phosphorylation ◽  
Schistosoma Japonicum ◽  
Phosphorylation Sites ◽  
Human Pathogen

scholarly journals Analysis of Flagellar Phosphoproteins from Chlamydomonas reinhardtii

2009 ◽  
Vol 8 (7) ◽  
pp. 922-932 ◽  
Author(s):  
Jens Boesger ◽  
Volker Wagner ◽  
Wolfram Weisheit ◽  
Maria Mittag
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Protein Phosphorylation ◽  
Proteome Analysis ◽  
Physiological Status ◽  
Phosphorylation Sites ◽  
Cell Organelles ◽  
Flagellar Proteins ◽  
Reversible Protein Phosphorylation ◽  
Cilia And Flagella

ABSTRACT Cilia and flagella are cell organelles that are highly conserved throughout evolution. For many years, the green biflagellate alga Chlamydomonas reinhardtii has served as a model for examination of the structure and function of its flagella, which are similar to certain mammalian cilia. Proteome analysis revealed the presence of several kinases and protein phosphatases in these organelles. Reversible protein phosphorylation can control ciliary beating, motility, signaling, length, and assembly. Despite the importance of this posttranslational modification, the identities of many ciliary phosphoproteins and knowledge about their in vivo phosphorylation sites are still missing. Here we used immobilized metal affinity chromatography to enrich phosphopeptides from purified flagella and analyzed them by mass spectrometry. One hundred forty-one phosphorylated peptides were identified, belonging to 32 flagellar proteins. Thereby, 126 in vivo phosphorylation sites were determined. The flagellar phosphoproteome includes different structural and motor proteins, kinases, proteins with protein interaction domains, and many proteins whose functions are still unknown. In several cases, a dynamic phosphorylation pattern and clustering of phosphorylation sites were found, indicating a complex physiological status and specific control by reversible protein phosphorylation in the flagellum.

High-accuracy identification and bioinformatic analysis of in vivo protein phosphorylation sites in yeast

PROTEOMICS ◽  
2009 ◽  
Vol 9 (20) ◽  
pp. 4642-4652 ◽  
Author(s):  
Florian Gnad ◽  
Lyris M. F. de Godoy ◽  
Jürgen Cox ◽  
Nadin Neuhauser ◽  
Shubin Ren ◽  
...  
Keyword(s):  
Protein Phosphorylation ◽  
Bioinformatic Analysis ◽  
High Accuracy ◽  
Phosphorylation Sites

scholarly journals PhosphoGRID: a database of experimentally verified in vivo protein phosphorylation sites from the budding yeast Saccharomyces cerevisiae

Database ◽  
2010 ◽  
Vol 2010 (0) ◽  
pp. bap026-bap026 ◽  
Author(s):  
C. Stark ◽  
T.-C. Su ◽  
A. Breitkreutz ◽  
P. Lourenco ◽  
M. Dahabieh ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Phosphorylation ◽  
Budding Yeast ◽  
Phosphorylation Sites ◽  
Yeast Saccharomyces Cerevisiae

scholarly journals In vivo phosphorylation sites in fetal and adult rat tau.

1993 ◽  
Vol 268 (34) ◽  
pp. 25712-25717
Author(s):  
A Watanabe ◽  
M Hasegawa ◽  
M Suzuki ◽  
K Takio ◽  
M Morishima-Kawashima ◽  
...  
Keyword(s):  
Phosphorylation Sites ◽  
Adult Rat

scholarly journals Genome‐wide analysis of in vivo CcpA binding with and without its key co‐factor HPr in the major human pathogen group A Streptococcus

2020 ◽  
Author(s):  
Sruti DebRoy ◽  
Victor Aliaga‐Tobar ◽  
Gabriel Galvez ◽  
Srishtee Arora ◽  
Xiaowen Liang ◽  
...  
Keyword(s):  
Group A Streptococcus ◽  
Human Pathogen ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Group A

scholarly journals Frequency- and Afterload-Dependent Cardiac Modulation In Vivo by Troponin I With Constitutively Active Protein Kinase A Phosphorylation Sites

2004 ◽  
Vol 94 (4) ◽  
pp. 496-504 ◽  
Author(s):  
Eiki Takimoto ◽  
David G. Soergel ◽  
Paul M.L. Janssen ◽  
Linda B. Stull ◽  
David A. Kass ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Troponin I ◽  
Phosphorylation Sites ◽  
Active Protein ◽  
Cardiac Modulation ◽  
Kinase A ◽  
Constitutively Active

In vivo praziquantel efficacy of Schistosoma japonicum over time: a systematic review and meta-analysis

Acta Tropica ◽  
2021 ◽  
pp. 106048
Author(s):  
Qiu-Fu Yu ◽  
Jie-Ying Zhang ◽  
Meng-Tao Sun ◽  
Man-Man Gu ◽  
Hui-Ying Zou ◽  
...  
Keyword(s):  
Systematic Review ◽  
Schistosoma Japonicum ◽  
Meta Analysis ◽  
Over Time
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