Non-invasive proteomic analysis of human skin keratins: Screening of methionine oxidation in keratins by mass spectrometry

2011 ◽  
Vol 75 (2) ◽  
pp. 435-449 ◽  
Author(s):  
Seon Hwa Lee ◽  
Kohei Miyamoto ◽  
Takaaki Goto ◽  
Tomoyuki Oe
Keyword(s):  
Mass Spectrometry ◽  
Human Skin ◽  
Proteomic Analysis ◽  
Methionine Oxidation ◽  
Non Invasive

scholarly journals Mass spectrometry data from proteomic analysis of human skin keratins after exposure to UV radiation

Data in Brief ◽  
2016 ◽  
Vol 7 ◽  
pp. 100-106 ◽  
Author(s):  
Seon Hwa Lee ◽  
Keita Matsushima ◽  
Kohei Miyamoto ◽  
Tomoyuki Oe
Keyword(s):  
Mass Spectrometry ◽  
Human Skin ◽  
Uv Radiation ◽  
Proteomic Analysis ◽  
Mass Spectrometry Data

scholarly journals Mass spectrometry‐based top‐down and bottom‐up approaches for proteomic analysis of the Moroccan Buthus occitanus scorpion venom

FEBS Open Bio ◽  
2021 ◽  
Author(s):  
Khadija Daoudi ◽  
Christian Malosse ◽  
Ayoub Lafnoune ◽  
Bouchra Darkaoui ◽  
Salma Chakir ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Proteomic Analysis ◽  
Scorpion Venom ◽  
Top Down ◽  
Bottom Up

scholarly journals Flexible Potentiometric Sensor System for Non-Invasive Determination of Antioxidant Activity of Human Skin: Application for Evaluating the Effectiveness of Phytocosmetic Products

Chemosensors ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 76
Author(s):  
Aleksey V. Tarasov ◽  
Ekaterina I. Khamzina ◽  
Maria A. Bukharinova ◽  
Natalia Yu. Stozhko
Keyword(s):  
Antioxidant Activity ◽  
Human Skin ◽  
Skin Diseases ◽  
Silver Chloride ◽  
Potentiometric Sensor ◽  
Sensor System ◽  
Non Invasive ◽  
Pet Film ◽  
The Impact

In contemporary bioanalysis, monitoring the antioxidant activity (AOA) of the human skin is used to assess stresses, nutrition, cosmetics, and certain skin diseases. Non-invasive methods for skin AOA monitoring have certain advantages over invasive methods, namely cost-effectiveness, lower labor intensity, reduced risk of infection, and obtaining results in the real-time mode. This study presents a new flexible potentiometric sensor system (FPSS) for non-invasive determination of the human skin AOA, which is based on flexible film electrodes (FFEs) and membrane containing a mediator ([Fe(CN)6]3–/4–). Low-cost available materials and scalable technologies were used for FFEs manufacturing. The indicator FFE was fabricated based on polyethylene terephthalate (PET) film and carbon veil (CV) by single-sided hot lamination. The reference FFE was fabricated based on PET film and silver paint by using screen printing, which was followed by the electrodeposition of precipitate containing a mixture of silver chloride and silver ferricyanide (SCSF). The three-electrode configuration of the FPSS, including two indicator FFEs (CV/PET) and one reference FFE (SCSF/Ag/PET), has been successfully used for measuring the skin AOA and evaluating the impact of phytocosmetic products. FPSS provides reproducible (RSD ≤ 7%) and accurate (recovery of antioxidants is almost 100%) results, which allows forecasting its broad applicability in human skin AOA monitoring as well as for evaluating the effectiveness of topically and orally applied antioxidants.

Mass spectrometry-based proteomic analysis of parathyroid adenomas reveals PTH as a new human hormone-derived amyloid fibril protein

Amyloid ◽  
2021 ◽  
pp. 1-5
Author(s):  
Magali Colombat ◽  
Béatrice Barres ◽  
Claire Renaud ◽  
David Ribes ◽  
Sarah Pericard ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Proteomic Analysis ◽  
Amyloid Fibril ◽  
Parathyroid Adenomas ◽  
Amyloid Fibril Protein

scholarly journals Proteomic Analyses of Vitreous in Proliferative Diabetic Retinopathy: Prior Studies and Future Outlook

2021 ◽  
Vol 10 (11) ◽  
pp. 2309
Author(s):  
Sarah R. Weber ◽  
Yuanjun Zhao ◽  
Christopher Gates ◽  
Jingqun Ma ◽  
Felipe da Veiga Leprevost ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Diabetic Retinopathy ◽  
Proliferative Diabetic Retinopathy ◽  
Proteomic Analysis ◽  
Retinal Disease ◽  
Vitreous Fluid ◽  
Future Outlook ◽  
Novel Proteins ◽  
Popular Medium ◽  
Molecular Information

Vitreous fluid is becoming an increasingly popular medium for the study of retinal disease. Numerous studies have demonstrated that proteomic analysis of the vitreous from patients with proliferative diabetic retinopathy yields valuable molecular information regarding known and novel proteins and pathways involved in this disease. However, there is no standardized methodology for vitreous proteomic studies. Here, we share a suggested protocol for such studies and outline the various experimental and analytic methods that are currently available. We also review prior mass spectrometry-based proteomic studies of the vitreous from patients with proliferative diabetic retinopathy, discuss common pitfalls of these studies, and propose next steps for moving the field forward.

Sign in / Sign up

Export Citation Format

Share Document