2-D difference gel electrophoresis approach to assess protein expression profiles in Bathymodiolus azoricus from Mid-Atlantic Ridge hydrothermal vents

2011 ◽  
Vol 74 (12) ◽  
pp. 2909-2919 ◽  
Author(s):  
Rui Company ◽  
Oreto Antúnez ◽  
Maria João Bebianno ◽  
Miren P. Cajaraville ◽  
Amparo Torreblanca
Keyword(s):  
Protein Expression ◽  
Gel Electrophoresis ◽  
Hydrothermal Vents ◽  
Expression Profiles ◽  
Difference Gel Electrophoresis ◽  
Mid Atlantic Ridge ◽  
Protein Expression Profiles

scholarly journals Calreticulin as A Novel Potential Metastasis-Associated Protein in Myxoid Liposarcoma, as Revealed by Two-Dimensional Difference Gel Electrophoresis

Proteomes ◽  
2019 ◽  
Vol 7 (2) ◽  
pp. 13
Author(s):  
Takashi Tajima ◽  
Fusako Kito ◽  
Akihiko Yoshida ◽  
Akira Kawai ◽  
Tadashi Kondo
Keyword(s):  
Gel Electrophoresis ◽  
Expression Profiles ◽  
Myxoid Liposarcoma ◽  
Clinical Applications ◽  
Two Dimensional ◽  
2D Dige ◽  
Tumor Tissues ◽  
Difference Gel Electrophoresis ◽  
Protein Expression Profiles

Myxoid liposarcoma (MLS) is a mesenchymal malignancy. To identify innovate seeds for clinical applications, we examined the proteomes of primary tumor tissues from 10 patients with MLS with different statuses of postoperative metastasis. The protein expression profiles of tumor tissues were created, and proteins with differential expression associated with postoperative metastasis were identified by two-dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry. The validation was performed using specific antibodies and in vitro analyses. Using 2D-DIGE, we observed 1726 protein species and identified proteins with unique expression levels in metastatic MLS. We focused on the overexpression of calreticulin in metastatic MLS. The higher expression of calreticulin was confirmed by Western blotting, and gene silencing assays demonstrated that reduced expression of calreticulin inhibited cell growth and invasion. Our findings suggested the important roles of calreticulin in MLS metastasis and supported its potential utility as a prognostic biomarker in MLS. Further investigations of the functional properties of calreticulin and other proteins identified in this study will improve our understanding of the biology of MLS and facilitate novel clinical applications.

P-652 Protein expression profiles in non-small cell lung cancer. A tissue microarray-based study of 142 cases

Lung Cancer ◽  
2005 ◽  
Vol 49 ◽  
pp. S290 ◽  
Author(s):  
E. Conde ◽  
R. García Luján ◽  
A. López Encuentra ◽  
L. Sánchez ◽  
M. Sánchez-Céspedes ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Protein Expression ◽  
Small Cell Lung Cancer ◽  
Tissue Microarray ◽  
Cell Lung Cancer ◽  
Expression Profiles ◽  
Small Cell ◽  
Small Cell Lung ◽  
Protein Expression Profiles

scholarly journals Brain death induces the alteration of liver protein expression profiles in rabbits

2014 ◽  
Vol 34 (2) ◽  
pp. 578-584 ◽  
Author(s):  
BING DU ◽  
LING LI ◽  
ZHIBIAO ZHONG ◽  
XIAOLI FAN ◽  
BINGBING QIAO ◽  
...  
Keyword(s):  
Protein Expression ◽  
Brain Death ◽  
Expression Profiles ◽  
Liver Protein ◽  
Protein Expression Profiles
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