Proteomic analysis of plasma samples from patients with acute myocardial infarction identifies haptoglobin as a potential prognostic biomarker

2011 ◽  
Vol 75 (1) ◽  
pp. 229-236 ◽  
Author(s):  
Benjamin Haas ◽  
Tommaso Serchi ◽  
Daniel R. Wagner ◽  
Georges Gilson ◽  
Sebastien Planchon ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Proteomic Analysis ◽  
Prognostic Biomarker ◽  
Plasma Samples

scholarly journals Full-Size and Partially Truncated Cardiac Troponin Complexes in the Blood of Patients with Acute Myocardial Infarction

2019 ◽  
Vol 65 (7) ◽  
pp. 882-892 ◽  
Author(s):  
Alexandra V Vylegzhanina ◽  
Alexander E Kogan ◽  
Ivan A Katrukha ◽  
Ekaterina V Koshkina ◽  
Anastasia V Bereznikova ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Troponin I ◽  
Troponin T ◽  
Gel Filtration ◽  
Free Form ◽  
Plasma Samples ◽  
Full Size ◽  
Troponin Complex ◽  
Almost All

AbstractBACKGROUNDThe measurement of cardiac isoforms of troponin I (cTnI) and troponin T (cTnT) is widely used for the diagnosis of acute myocardial infarction (AMI). However, there are conflicting data regarding what forms of cTnI and cTnT are present in the blood of AMI patients. We investigated cTnI and cTnT as components of troponin complexes in the blood of AMI patients.METHODSGel filtration techniques, sandwich fluoroimmunoassays, and Western blotting were used.RESULTSPlasma samples from patients with AMI contained the following troponin complexes: (a) a cTnI-cTnT-TnC complex (ITC) composed of full-size cTnT of 37 kDa or its 29-kDa fragment and full-size cTnI of 29 kDa or its 27-kDa fragments; (b) ITC with lower molecular weight (LMW-ITC) in which cTnT was truncated to the 14-kDa C-terminal fragments; and (c) a binary cTnI-cTnC complex composed of truncated cTnI of approximately 14 kDa. During the progression of the disease, the amount of ITC in AMI samples decreased, whereas the amounts of LMW-ITC and short 16- to 20-kDa cTnT central fragments increased. Almost all full-size cTnT and a 29-kDa cTnT fragment in AMI plasma samples were the components of ITC. No free full-size cTnT was found in AMI plasma samples. Only 16- to 27-kDa central fragments of cTnT were present in a free form in patient blood.CONCLUSIONSA ternary troponin complex exists in 2 forms in the blood of patients with AMI: full-size ITC and LMW-ITC. The binary cTnI-cTnC complex and free cTnT fragments are also present in patient blood.

scholarly journals Circulating miR-30a-5p as a prognostic biomarker of left ventricular dysfunction after acute myocardial infarction

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Agata Maciejak ◽  
Edyta Kostarska-Srokosz ◽  
Wlodzimierz Gierlak ◽  
Miroslaw Dluzniewski ◽  
Marek Kuch ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Left Ventricular Dysfunction ◽  
Ventricular Dysfunction ◽  
Prognostic Biomarker ◽  
Left Ventricular

Molecular imprinting coupled with electrochemical analysis for plasma samples classification in acute myocardial infarction diagnostic

2018 ◽  
Vol 99 ◽  
pp. 216-222 ◽  
Author(s):  
Victoria V. Shumyantseva ◽  
Tatiana V. Bulko ◽  
Larisa V. Sigolaeva ◽  
Alexey V. Kuzikov ◽  
Pavel V. Pogodin ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Molecular Imprinting ◽  
Electrochemical Analysis ◽  
Plasma Samples

scholarly journals Circulating MiR-19b-3p, MiR-134-5p and MiR-186-5p are Promising Novel Biomarkers for Early Diagnosis of Acute Myocardial Infarction

2016 ◽  
Vol 38 (3) ◽  
pp. 1015-1029 ◽  
Author(s):  
Ke-Jing Wang ◽  
Xin Zhao ◽  
Yu-Zhou Liu ◽  
Qiu-Tang Zeng ◽  
Xiao-Bo Mao ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Troponin I ◽  
Early Stage ◽  
Diagnostic Efficiency ◽  
Plasma Samples ◽  
Circulating Mirnas ◽  
Peak Expression ◽  
And Gender ◽  
The Impact

Background/Aims: Recent studies have shown that circulating microRNAs (miRNAs) are emerging as promising biomarkers for cardiovascular diseases. This study aimed to determine whether miR-19b-3p, miR-134-5p and miR-186-5p can be used as novel indicators for acute myocardial infarction (AMI). Methods: To investigate the kinetic expression of the three selected miRNAs, we enrolled 18 patients with AMI and 20 matched controls. Plasma samples were collected from each participant, and total RNA was extracted. Quantitative real-time PCR and ELISA assays were used to investigate the expression of circulating miRNAs and cardiac troponin I (cTnI), respectively. Plasma samples from another age- and gender-matched cohort were collected to investigate the impact of medications for AMI on the expression of the selected miRNAs. Results: Levels of plasma miR-19b-3p, miR-134-5p and miR-186-5p were significantly increased in early stage of AMI. Plasma miR-19b-3p and miR-134-5p levels reached peak expression immediately after admission (T0), whereas miR-186-5p achieved peak expression at 4 h after T0. All of these times were earlier than the peak for cTnI (8 h after T0). In addition, all three miRNAs were positively correlated with cTnI. Receiver Operating Characteristic (ROC) analysis indicated that each single miRNA showed considerable diagnostic efficiency for predicting AMI. Furthermore, combining all three miRNAs in a panel increased the efficiency of distinguishing between patients with AMI and controls. Moreover, we found that heparin and medications for AMI did not impact the expression of these circulating miRNAs. Conclusion: Circulating miR-19b-3p, miR-134-5p and miR-186-5p could be considered promising novel diagnostic biomarkers for the early phase of AMI.

Plasma-Mediated Neutrophil Activation during Acute Myocardial Infarction: Role of Platelet-Activating Factor

1995 ◽  
Vol 89 (2) ◽  
pp. 171-176 ◽  
Author(s):  
Tomasz Siminiak ◽  
Robin M. Egdell ◽  
Daniel J. O'Gorman ◽  
Julian F. Dye ◽  
Desmond J. Sheridan
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Superoxide Anion ◽  
Platelet Activating Factor ◽  
Neutrophil Activation ◽  
Polymorphonuclear Neutrophils ◽  
Polymorphonuclear Neutrophil ◽  
Plasma Samples ◽  
Superoxide Anion Production ◽  
Anion Production

1. Polymorphonuclear neutrophils are involved in the development of myocardial injury during ischaemia through the release of free oxygen radicals and by adhesion of activated polymorphonuclear neutrophils to endothelium, resulting in plugging of coronary capillaries. Polymorphonuclear neutrophil activation may be a result of contact with ligands expressed by endothelial cells and/or a response to soluble stimuli released from ischaemic tissue to the plasma. 2. To investigate this we studied plasma-mediated polymorphonuclear neutrophil activation in vitro using plasma samples collected from 14 patients with acute myocardial infarction at time of admission and 6 h and 1, 2, 5 and 7 days later. Plasma samples were incubated with washed polymorphonuclear neutrophils isolated from healthy donors. Expression of adhesion molecules CD18/CD11b integrin and L-selectin (Leu-8) were measured by flow cytometry and superoxide anion production in polymorphonuclear neutrophils was measured by chemiluminescence. 3. Plasma samples obtained 6 h and 1 day after admission were capable of inducing CD18/CD11b antigen expression, superoxide anion production and L-selectin shedding in the washed polymorphonuclear neutrophils, and this effect was significant when compared with plasma taken at 5 and 7 days after admission. 4. The plasma-mediated polymorphonuclear neutrophil stimulation was prevented when the PMN were pretreated with platelet-activating factor receptor antagonists BN52021 or BN50739. The platelet-activating factor concentrations detected in the plasma samples were not higher than those detected in plasma from healthy subjects. 5. These findings suggest that during acute myocardial infarction peripheral plasma contains soluble stimuli capable of inducing polymorphonuclear neutrophil integrin expression, L-selectin shedding and oxygen free radical production and that platelet-activating factor appears to act as an autocrine polymorphonuclear neutrophil stimulus.

Cystatin C as Prognostic Biomarker in ST-Segment Elevation Acute Myocardial Infarction

2012 ◽  
Vol 109 (10) ◽  
pp. 1431-1438 ◽  
Author(s):  
Doroteia Silva ◽  
Nuno Cortez-Dias ◽  
Cláudia Jorge ◽  
J. Silva Marques ◽  
Pedro Carrilho-Ferreira ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Cystatin C ◽  
Prognostic Biomarker ◽  
St Segment Elevation ◽  
St Segment ◽  
Elevation Acute Myocardial Infarction

Copeptin as a prognostic biomarker in acute myocardial infarction

2019 ◽  
Vol 274 ◽  
pp. 337-341 ◽  
Author(s):  
Benoit Lattuca ◽  
Vuthy Sy ◽  
Lee S. Nguyen ◽  
Maguy Bernard ◽  
Michel Zeitouni ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Prognostic Biomarker
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