MdVHA-A encodes an apple subunit A of vacuolar H+-ATPase and enhances drought tolerance in transgenic tobacco seedlings

2013 ◽  
Vol 170 (6) ◽  
pp. 601-609 ◽  
Author(s):  
Qing-Long Dong ◽  
Chun-Rong Wang ◽  
Dan-Dan Liu ◽  
Da-Gang Hu ◽  
Mou-Jing Fang ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
Subunit A ◽  
Tobacco Seedlings

scholarly journals Overexpression of the pitaya phosphoethanolamine N-methyltransferase gene (HpPEAMT) enhanced simulated drought stress in tobacco

2021 ◽  
Author(s):  
Ai-Hua Wang ◽  
Lan Yang ◽  
Xin-Zhuan Yao ◽  
Xiao-Peng Wen
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Transgenic Plants ◽  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
Sequence Similarity ◽  
Amino Acid Sequence Similarity ◽  
Transgenic Tobacco Plants ◽  
Wild Type ◽  
Tobacco Plants

AbstractPhosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.

Leaf-specific overexpression of plastidic glutamine synthetase stimulates the growth of transgenic tobacco seedlings

Planta ◽  
2000 ◽  
Vol 210 (2) ◽  
pp. 252-260 ◽  
Author(s):  
Andrea Migge ◽  
Elisa Carrayol ◽  
Bertrand Hirel ◽  
Thomas W. Becker
Keyword(s):  
Glutamine Synthetase ◽  
Transgenic Tobacco ◽  
Tobacco Seedlings

A novel LEA gene from Tamarix androssowii confers drought tolerance in transgenic tobacco

Plant Science ◽  
2006 ◽  
Vol 171 (6) ◽  
pp. 655-662 ◽  
Author(s):  
Yucheng Wang ◽  
Jing Jiang ◽  
Xin Zhao ◽  
Guifeng Liu ◽  
Chuanping Yang ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
Lea Gene

scholarly journals Overexpression of a Voltage-Dependent Anion-Selective Channel (VDAC) Protein-Encoding Gene, MsVDAC, from Medicago sativa Confers Cold and Drought Tolerance to Transgenic Tobacco

Genes ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1706
Author(s):  
Mei Yang ◽  
Xinhang Duan ◽  
Zhaoyu Wang ◽  
Hang Yin ◽  
Junrui Zang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Cold Stress ◽  
Medicago Sativa ◽  
Transgenic Tobacco ◽  
Soluble Sugars ◽  
Ros Scavenging ◽  
Osmotic Homeostasis ◽  
Voltage Dependent ◽  
Stress Responsive Genes

Voltage-dependent anion channels (VDACs) are highly conserved proteins that are involved in the translocation of tRNA and play a key role in modulating plant senescence and multiple pathways. However, the functions of VDACs in plants are still poorly understood. Here, a novel VDAC gene was isolated and identified from alfalfa (Medicago sativa L.). MsVDAC localized to the mitochondria, and its expression was highest in alfalfa roots and was induced in response to cold, drought and salt treatment. Overexpression of MsVDAC in tobacco significantly increased MDA, GSH, soluble sugars, soluble protein and proline contents under cold and drought stress. However, the activities of SOD and POD decreased in transgenic tobacco under cold stress, while the O2− content increased. Stress-responsive genes including LTP1, ERD10B and Hxk3 were upregulated in the transgenic plants under cold and drought stress. However, GAPC, CBL1, BI-1, Cu/ZnSOD and MnSOD were upregulated only in the transgenic tobacco plants under cold stress, and GAPC, CBL1, and BI-1 were downregulated under drought stress. These results suggest that MsVDAC provides cold tolerance by regulating ROS scavenging, osmotic homeostasis and stress-responsive gene expression in plants, but the improved drought tolerance via MsVDAC may be mainly due to osmotic homeostasis and stress-responsive genes.

Drought tolerance through overexpression of monoubiquitin in transgenic tobacco

2008 ◽  
Vol 165 (16) ◽  
pp. 1745-1755 ◽  
Author(s):  
Qifang Guo ◽  
Jin Zhang ◽  
Qiang Gao ◽  
Shichao Xing ◽  
Feng Li ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Transgenic Tobacco
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