scholarly journals Comparative analysis of constitutes and metabolites for traditional Chinese medicine using IDA and SWATH data acquisition modes on LC-Q-TOF MS

2020 ◽  
Vol 10 (6) ◽  
pp. 588-596
Author(s):  
Dian Kang ◽  
Qingqing Ding ◽  
Yangfan Xu ◽  
Xiaoxi Yin ◽  
Huimin Guo ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Data Acquisition ◽  
Tof Ms

scholarly journals The Correlation between Chemical Composition, as Determined by UPLC-TOF-MS, and Acute Toxicity ofVeratrum nigrumL. andRadix paeoniae alba

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Xianxie Zhang ◽  
Yuguang Wang ◽  
Qiande Liang ◽  
Zengchun Ma ◽  
Chengrong Xiao ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Acute Toxicity ◽  
Chemical Constituents ◽  
Two Agents ◽  
Veratrum Alkaloids ◽  
Radix Paeoniae Alba ◽  
Insight Into ◽  
Tof Ms

The eighteen incompatible medicaments is an important theory in traditional Chinese medicine. The theory suggests that drugs in the eighteen incompatible medicaments can be toxic when used together.Veratrum nigrumL. andRadix paeoniae albabelong to the eighteen incompatible medicaments and have been prohibited for thousands of years. This study offers preliminary insight into the mechanism and chemical constituents responsible for the incompatibility and toxicity of these two agents. Specifically, we performed toxicology studies to identify and quantify the constituent substances of the two agents. Experiments revealed that acute toxicity increases when the dose ofV. nigrumL. is higher than, or equal to, RPA. UPLC-TOF-MS analysis showed that, although the volumes ofV. nigrumL. were the same, the content of some veratrum alkaloids changed significantly and had a trend toward a highly positive correlationr≥0.8with toxicity. This suggests that the increased toxicity of theV. nigrumL. and RPA combination was due mainly to increased content of the special veratrum alkaloids. The cytotoxicity of veratridine in SH-SY5Y cells was decreased with increasing paeoniflorin concentrations. This study provides insight into the mechanism behind the incompatibility theory of TCM.

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