In vitro evaluation of the biological activities of IgG in seven Chinese intravenous immunoglobulin preparations

2018 ◽  
Vol 151 ◽  
pp. 317-323 ◽  
Author(s):  
Shengliang Ye ◽  
Dong Li ◽  
Fengjuan Liu ◽  
Min Lei ◽  
Peng Jiang ◽  
...  
Keyword(s):  
Intravenous Immunoglobulin ◽  
Biological Activities ◽  
In Vitro Evaluation ◽  
Immunoglobulin Preparations

In vitro evaluation of biological activities of Orobanche crenata Forssk. leaves extract

2019 ◽  
Vol 34 (22) ◽  
pp. 3234-3238 ◽  
Author(s):  
Carlo Genovese ◽  
Rosaria Acquaviva ◽  
Simone Ronsisvalle ◽  
Gianna Tempera ◽  
Giuseppe Antonio Malfa ◽  
...  
Keyword(s):  
Biological Activities ◽  
Orobanche Crenata ◽  
In Vitro Evaluation

Vasoactive side effects of intravenous immunoglobulin preparations in a rat model and their treatment with recombinant platelet-activating factor acetylhydrolase

Blood ◽  
2000 ◽  
Vol 95 (5) ◽  
pp. 1856-1861 ◽  
Author(s):  
Wim K. Bleeker ◽  
Jessica L. Teeling ◽  
Arthur J. Verhoeven ◽  
Gemma M. M. Rigter ◽  
Jacques Agterberg ◽  
...  
Keyword(s):  
Side Effects ◽  
Intravenous Immunoglobulin ◽  
Rat Model ◽  
Platelet Activating Factor ◽  
Human Neutrophils ◽  
Ivig Infusion ◽  
Platelet Activating Factor Acetylhydrolase ◽  
Paf Acetylhydrolase ◽  
Immunoglobulin Preparations

Previously, we observed in a rat model that intravenous administration of intramuscular immunoglobulin preparations induced a long-lasting hypotension, which appeared to be associated with the presence of IgG polymers and dimers in the preparations, but unrelated to complement activation. We found evidence that this hypotensive response is mediated by platelet-activating factor (PAF) produced by macrophages. In this study, we compared the vasoactive effects of 16 intravenous immunoglobulin (IVIG) products from 10 different manufacturers, in anesthetized rats. Eight of the IVIG preparations showed no hypotensive effects (less than 15% decrease), whereas the other 8 had relatively strong effects (15%-50% decrease). The hypotensive effects correlated with the IgG dimer content of the preparations. Pretreatment of the rats with recombinant PAF acetylhydrolase completely prevented the hypotensive reaction on IVIG infusion, and administration after the onset of hypotension resulted in normalization of the blood pressure. We also observed PAF production on in vitro incubation of human neutrophils with IVIG, which could be blocked by anti-Fcγ receptor antibodies. This indicates that induction of PAF generation may also occur in a human system. Our findings support the hypothesis that the clinical side effects of IVIG in patients may be caused by macrophage and neutrophil activation through interaction of IgG dimers with Fcγ receptors. Because phagocyte activation may also lead to the release of other inflammatory mediators, recombinant PAF acetylhydrolase (rPAF-AH) provides a useful tool to determine whether PAF plays a role in the clinical side effects of IVIG. If so, rPAF-AH can be used for the treatment of those adverse reactions.

Immunoglobulin M–Enriched Human Intravenous Immunoglobulin Prevents Complement Activation In Vitro and In Vivo in a Rat Model of Acute Inflammation

Blood ◽  
1999 ◽  
Vol 93 (3) ◽  
pp. 942-951 ◽  
Author(s):  
Robert Rieben ◽  
Anja Roos ◽  
Yvonne Muizert ◽  
Caroline Tinguely ◽  
Arnout F. Gerritsen ◽  
...  
Keyword(s):  
Intravenous Immunoglobulin ◽  
Complement Activation ◽  
Inhibitory Activity ◽  
Solid Phase ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Glomerular Mesangial Cells ◽  
Immunoglobulin Preparations

An important antiinflammatory mechanism of intravenous immunoglobulin preparations (IVIG) is their ability to block complement activation. The purpose of this study was to compare the complement-inhibitory activity of four IVIG preparations differing in isotype composition. The preparations were: (1) IVIgG (48 g/L IgG, 2 g/L IgA; Intraglobin F); (2) Pentaglobin (38 g/L IgG, 6 g/L IgM, 6 g/L IgA); (3) IVIgM (35 g/L IgM, 12 g/L IgA, 3 g/L IgG); and (4) IVIgA (41 g/L IgA, 9 g/L IgG), all from Biotest Pharma GmbH, Dreieich, Germany. Their complement inhibitory activity was assessed in vitro by measurement of the blocking of C1q-, C4-, and C3 deposition on solid-phase aggregated rabbit IgG by enzyme-linked immunosorbent assay (ELISA). Complement inhibition in this ELISA was best for IVIgM, followed by Pentaglobin and IVIgG; IVIgA did not exhibit an inhibitory effect. Control experiments with excess concentrations of C1q as well as with C1q-depleted serum showed that the inhibitory effects of IVIG were not caused by complement activation and thus, consumption, but that C4 and C3 were scavenged by IgM and to a lesser extent by IgG. These results were confirmed in vivo in the rat anti-Thy 1 nephritis model, in which a single dose of 500 mg/kg of IVIgM prevented C3-, C6-, and C5b-9 deposition in the rat glomeruli, whereas the effect of IVIgG was much less pronounced. Reduction of complement deposition was paralleled by a diminished albuminuria, which was completely absent in the IVIgM-treated rats. IVIgM and to a lesser extent IVIgG also prevented rat C3 deposition on cultured rat glomerular mesangial cells in vitro, but did not influence anti-Thy 1 binding. Neither IVIgM nor Pentaglobin nor IVIgG negatively affected in vitro phagocytosis of Escherichia coli (E coli) by human granulocytes. In conclusion, we have shown that IgM enrichment of IVIG preparations enhances their effect to prevent the inflammatory effects of complement activation.

In vitro evaluation of anticancer and biological activities of synthesized manganese oxide nanoparticles

MedChemComm ◽  
2016 ◽  
Vol 7 (8) ◽  
pp. 1647-1653 ◽  
Author(s):  
Shahanavaj Khan ◽  
Anees A. Ansari ◽  
Abdul Arif Khan ◽  
Maha Abdulla ◽  
Omar Al- Obeed ◽  
...  
Keyword(s):  
Biological Activity ◽  
Manganese Oxide ◽  
Systematic Study ◽  
Biological Activities ◽  
Oxide Nanoparticles ◽  
Manganese Oxide Nanoparticles ◽  
In Vitro Evaluation

This paper presents the results from a systematic study into the characterization and anticancer and biological activity of synthesized super-paramagnetic manganese oxide nanoparticles (Mn3O4-NPs).

scholarly journals 4CPS-100 Quality of intratect: in vitro evaluation of biological activities

2019 ◽  
Author(s):  
C Schmidt ◽  
K Winterling ◽  
CC Heinz ◽  
M König ◽  
V Braun ◽  
...  
Keyword(s):  
Biological Activities ◽  
In Vitro Evaluation

scholarly journals In vitro and in vivo properties differ among liquid intravenous immunoglobulin preparations

Vox Sanguinis ◽  
2012 ◽  
Vol 104 (2) ◽  
pp. 115-126 ◽  
Author(s):  
F. Dhainaut ◽  
P.‐O. Guillaumat ◽  
H. Dib ◽  
G. Perret ◽  
A. Sauger ◽  
...  
Keyword(s):  
Intravenous Immunoglobulin ◽  
Immunoglobulin Preparations
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