A highly sensitive LC–MS/MS method for determination of ketoconazole in human plasma: Application to a clinical study of the exposure to ketoconazole in patients after topical administration

2016 ◽  
Vol 128 ◽  
pp. 504-509 ◽  
Author(s):  
Keli Wang ◽  
Yao Wu ◽  
Zhiyan Chi ◽  
Chang Shu ◽  
Lingjun Li ◽  
...  
Keyword(s):  
Clinical Study ◽  
Human Plasma ◽  
Topical Administration ◽  
Highly Sensitive

Highly sensitive determination of alendronate in human plasma and dialysate using metal-free HPLC-MS/MS

2018 ◽  
Vol 30 ◽  
pp. 14-20 ◽  
Author(s):  
Miho Yamada ◽  
Xiao-Pen Lee ◽  
Masaya Fujishiro ◽  
Ken Iseri ◽  
Makoto Watanabe ◽  
...  
Keyword(s):  
Human Plasma ◽  
Metal Free ◽  
Highly Sensitive ◽  
Sensitive Determination

Reversed phase UPLC/APCI-MS determination of Vitamin K1 and menaquinone-4 in human plasma: Application to a clinical study

2020 ◽  
Vol 183 ◽  
pp. 113147 ◽  
Author(s):  
Yuning Zhang ◽  
Yashpal S. Chhonker ◽  
Veenu Bala ◽  
Alex Hagg ◽  
Linda G. Snetselaar ◽  
...  
Keyword(s):  
Clinical Study ◽  
Human Plasma ◽  
Reversed Phase ◽  
Vitamin K1

scholarly journals Determination of nimodipine in plasma by HPLC-MS/MS and pharmacokinetic application

2010 ◽  
Vol 46 (4) ◽  
pp. 665-677 ◽  
Author(s):  
Demétrius Fernandes do Nascimento ◽  
Manoel Odorico de Moraes ◽  
Fernando Antônio Frota Bezerra ◽  
Andréa Vieira Pontes ◽  
Célia Regina Amaral Uchoa ◽  
...  
Keyword(s):  
Human Plasma ◽  
Internal Standard ◽  
Pharmacokinetic Profile ◽  
Plasma Samples ◽  
Linear Calibration ◽  
Limit Of Quantification ◽  
Standard Curve ◽  
Liquid Liquid Extraction ◽  
Highly Sensitive

To develop and validate a rapid, specific and highly sensitive method to quantify nimodipine in human plasma using dibucaine as the internal standard (IS). The analyte and IS were extracted from plasma samples by liquid-liquid extraction using hexane-ethyl acetate (1:1 v/v). The chromatographic separation was performed on a Varian® Polaris C18 analytical column (3 μm, 50 x 2.0 mm) and pre-column SecurityguardTM C18 (4.0 x 3.0 mm) with a mobile phase of Acetonitrile-Ammonium acetate 0.02 ml/L (80:20v/v). The method had a chromatographic run time of 4.5 min and linear calibration curve over the range of 0.1- 40 ng/mL (r > 0.9938). The limit of quantification was 100 pg/mL. Acceptable precision and accuracy were obtained for concentrations over the standard curve ranges. This validated method was successfully applied in determining the pharmacokinetic profile of nimodipine tablets of 30 mg administered to 24 healthy volunteers. The proposed method of analysis provided a sensitive and specific assay for nimodipine determination in human plasma. The time for the determination of one plasma sample was 4.5 min. This method is suitable for the analysis of nimodipine in human plasma samples collected for pharmacokinetic, bioavailability or bioequivalence studies in humans.

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