Comparison of ultrasound-enhanced air-assisted liquid–liquid microextraction and low-density solvent-based dispersive liquid–liquid microextraction methods for determination of nonsteroidal anti-inflammatory drugs in human urine samples

2015 ◽  
Vol 111 ◽  
pp. 297-305 ◽  
Author(s):  
Behruz Barfi ◽  
Alireza Asghari ◽  
Maryam Rajabi ◽  
Ahmad Goochani Moghadam ◽  
Nasim Mirkhani ◽  
...  
Keyword(s):  
Human Urine ◽  
Urine Samples ◽  
Low Density ◽  
Human Urine Samples ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

scholarly journals Analysis of chlorpheniramine in human urine samples using dispersive liquid-liquid microextraction combined with high-performance liquid chromatography

2014 ◽  
Vol 50 (3) ◽  
pp. 551-557 ◽  
Author(s):  
Mehdi Maham ◽  
Vahid Kiarostami ◽  
Syed Waqif-Husain ◽  
Malihe Khoeini Sharifabadi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Urine ◽  
High Performance ◽  
Low Cost ◽  
Test Tube ◽  
Urine Samples ◽  
Human Urine Samples ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

A simple and environmentally friendly microextraction technique was used for determination of chlorpheniramine (CPM), an antihistamine drug, in human urine samples using dispersive liquid-liquid microextraction (DLLME) followed by high performance liquid chromatography with diode array detection (HPLC-DAD). In this extraction technique, an appropriate mixture of acetonitrile (disperser solvent) and carbon tetrachloride (extraction solvent) was rapidly injected into the urine sample containing the target analyte. Tiny droplets of extractant were formed and dispersed into the sample solution and then sedimented at the bottom of the conical test tube by centrifugation. Under optimal conditions, the calibration curve was linear in the range of 0.055-5.5 µg mL-1, with a detection limit of 16.5 ng mL-1. This proposed method was successfully applied to the analysis of real urine samples. Low consumption of toxic organic solvents, simplicity of operation, low cost and acceptable figures of merit are the main advantages of the proposed technique.

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