Simultaneous quantitative determination of 9 active components in traditional Chinese medicinal preparation ShuangDan oral liquid by RP-HPLC coupled with photodiode array detection

2011 ◽  
Vol 56 (4) ◽  
pp. 820-824 ◽  
Author(s):  
Hua Li ◽  
Si-Wang Wang ◽  
Bang-Le Zhang ◽  
Yan-Hua Xie ◽  
Qian Yang ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Medicinal Preparation ◽  
Active Components ◽  
Photodiode Array Detection ◽  
Rp Hplc ◽  
Oral Liquid ◽  
Photodiode Array ◽  
Array Detection

scholarly journals Quantitative Determination of Pregnanes from Aerial Parts of Caralluma Species Using HPLC-UV and Identification by LC-ESI-TOF

2011 ◽  
Vol 94 (5) ◽  
pp. 1383-1390
Author(s):  
Bharathi Avula ◽  
Yatin J Shukla ◽  
Yan-Hong Wang ◽  
Ikhlas A Khan
Keyword(s):  
Quantitative Determination ◽  
Dietary Supplements ◽  
Hplc Method ◽  
Photodiode Array Detection ◽  
Plant Materials ◽  
Aerial Parts ◽  
Photodiode Array ◽  
Array Detection ◽  
Na Ions

Abstract An HPLC method was developed for the quantitative determination of five pregnane derivatives from aerial parts of Caralluma species and dietary supplements. The method was validated for linearity, repeatability, LOD, and LOQ. The LOD and LOQ of five pregnane compounds were found to be in the range of 1–5 and 3–15 μg/mL, respectively, by HPLC using photodiode array detection. This method was applied to the identification of three plant materials of Caralluma species (C. fimbriata, C. umbellate, and C. attentuata) and seven dietary supplements claiming to contain C. fimbriata. An LC/MS coupled with electrospray ionization interface method was used for the identification of compounds and involved the use of [M+Na]+ ions in the positive ion mode with extracted ion chromatogram.

scholarly journals Development and Validation of an RP-HPLC-PDA Method for Determination of Paracetamol, Caffeine and Tramadol Hydrochloride in Pharmaceutical Formulations

2021 ◽  
Vol 14 (5) ◽  
pp. 466
Author(s):  
Fernando J. Pereira ◽  
Aida Rodríguez-Cordero ◽  
Roberto López ◽  
Luis C. Robles ◽  
A. Javier Aller
Keyword(s):  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Fluorescence Detector ◽  
Photodiode Array Detection ◽  
Tramadol Hydrochloride ◽  
Rp Hplc ◽  
Photodiode Array ◽  
Array Detection ◽  
Tablet Dosage

Paracetamol (acetaminophen) (PAR), caffeine (CAF) and tramadol hydrochloride (TRA) are important drugs widely used for many clinical purposes. Determination of their contents is of the paramount interest. In this respect, a quick, simple and sensitive isocratic RP-HPLC method with photodiode array detection was developed for the determination of paracetamol, caffeine and tramadol in pharmaceutical formulations. An improved sensitive procedure was also evolved for tramadol using a fluorescence detector system. A C18 column and a mobile phase constituted by methanol/phosphate were used. LODs were found to be 0.2 μg/mL, 0.1 μg/mL and 0.3 μg/mL for paracetamol, caffeine and tramadol hydrochloride, respectively, using photodiode-array detection. Alternatively, LOD for tramadol decreased to 0.1 μg/mL with the fluorescence detector. Other notable analytical figures of merit include the linear concentration ranges, 0.8–270 μg/mL, 0.4–250 μg/mL and 1.0–300 (0.2–40) μg/mL, for the same ordered analytes (including the fluorescence detector). The proposed method was successfully applied for the quantitative determination of the three drugs in tablet dosage forms.

Quantitative Determination of 14 Major Constituents in the Herbal Preparation Luan-Pao-Prescription Using HPLC Coupled with Photodiode Array Detection

2007 ◽  
Vol 66 (3-4) ◽  
pp. 267-270 ◽  
Author(s):  
Jin-qiang Zhang ◽  
Xiao-ming Wang ◽  
Zhi-qiang Lu ◽  
Hui-lian Huang ◽  
Guang-tong Chen ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Herbal Preparation ◽  
Photodiode Array Detection ◽  
Photodiode Array ◽  
Array Detection
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