Reliable on-line sample preparation of basic compounds from plasma using a reversed phase restricted access media in column-switching LC

2006 ◽  
Vol 42 (5) ◽  
pp. 587-592 ◽  
Author(s):  
Eiichi Yamamoto ◽  
Hatsue Igarashi ◽  
Yoshiaki Sato ◽  
Ikuo Kushida ◽  
Takashi Kato ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Column Switching ◽  
Reversed Phase ◽  
Restricted Access ◽  
Basic Compounds ◽  
Restricted Access Media ◽  
On Line ◽  
Line Sample

Rapid monitoring of benzodiazepines in clinical samples by using on-line column switching HPLC

1991 ◽  
Vol 37 (6) ◽  
pp. 804-808 ◽  
Author(s):  
C M Moore ◽  
K Sato ◽  
Y Katsumata
Keyword(s):  
Signal To Noise Ratio ◽  
Column Switching ◽  
Reversed Phase ◽  
Clinical Samples ◽  
Analytical Column ◽  
Drug Concentrations ◽  
Analytical Recovery ◽  
Rapid Monitoring ◽  
On Line ◽  
Line Sample

Abstract We have determined routinely administered benzodiazepines and some of their metabolites in clinical samples by using on-line column switching for sample clean-up and reversed-phase high-pressure liquid chromatography (HPLC). The plasma sample is directly injected onto a cyanopropyl (CN) pre-column, washed with buffer, and eluted onto an analytical column (C18, octadecylsilane). Analytical recovery from drug-supplemented plasma samples was greater than 85% for all the benzodiazepines tested. The response of the analytical column varied linearly with drug concentrations over the range 0.01-1 mg/L in plasma, with CVs ranging from 0.3% to 1.8% within-day and 5.5% to 15.7% between days. The detection limit (signal-to-noise ratio greater than 3) was 0.01 mg/L (1 ng on column) for all of the benzodiazepines and their metabolites. The advantages of the technique include the extreme cleanliness of the traces, no requirement for off-line sample preparation, and ease of automation.

scholarly journals On-line Sample Preparation System Using Column-Switching HPLC for the Structure Elucidation of Compounds in Mixtures by NMR.

2000 ◽  
Vol 16 (11) ◽  
pp. 1183-1188 ◽  
Author(s):  
Yumi YOKOYAMA ◽  
Naoya KISHI ◽  
Masayuki TANAKA ◽  
Naoki ASAKAWA
Keyword(s):  
Sample Preparation ◽  
Structure Elucidation ◽  
Column Switching ◽  
On Line ◽  
Line Sample
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