Adsorptive cathodic stripping voltammetric assay of the estrogen drug ethinylestradiol in pharmaceutical formulation and human plasma at a mercury electrode

2006 ◽  
Vol 40 (2) ◽  
pp. 255-261 ◽  
Author(s):  
E.M. Ghoneim ◽  
H.S. El-Desoky ◽  
M.M. Ghoneim
Keyword(s):  
Human Plasma ◽  
Mercury Electrode ◽  
Pharmaceutical Formulation ◽  
Voltammetric Assay ◽  
Cathodic Stripping

Voltammetric studies and assay of the anti-inflammatory drug ketoprofen in pharmaceutical formulation and human plasma at a mercury electrode

2003 ◽  
Vol 81 (8) ◽  
pp. 889-896 ◽  
Author(s):  
M M Ghoneim ◽  
A Tawfik
Keyword(s):  
Cyclic Voltammetry ◽  
Detection Limit ◽  
Human Plasma ◽  
Mercury Electrode ◽  
Pharmaceutical Formulation ◽  
Inflammatory Drug ◽  
Square Wave ◽  
Anti Inflammatory ◽  
Bulk Drug ◽  
Cathodic Stripping

The electrochemical reduction of the anti-inflammatory drug ketoprofen was studied in a Britton-Robinson (B-R.) buffer series of pH 2–11 using dc-polarography, cyclic voltammetry, and coulometry techniques. The electrode reaction pathway of the drug at the dropping mercury electrode was proposed and discussed. A new adsorptive cathodic stripping square-wave voltammetric procedure was optimized for the assay of bulk drug in a B-R. buffer of pH 2.0. The peak current was linear with the drug concentration over the ranges 2 × 10–9 to 2 × 10–7 M of the bulk drug, using a 60 s accumulation time period at –0.6 V (vs. Ag/AgCl/KCls). The percentage recovery of the bulk drug was 99.57 ± 0.54 and a detection limit of 0.10 ng mL–1 was achieved. The proposed procedure was successfully applied for the assay of ketoprofen in pharmaceutical formulation (Ketofan®) and human plasma. The percentage recoveries were 99.66 ± 0.47 and 101.76 ± 0.64 in pharmaceutical formulation and human plasma, respectively. A detection limit of 0.14 ng mL–1 plasma was achieved which was below that reported in literature using the different analytical techniques.Key words: ketoprofen (Ketofan®) determination, polarography, cyclic voltammetry, adsorptive cathodic stripping square-wave voltammetry, human plasma.

scholarly journals Mechanistic analysis of the cathodic stripping square-wave voltammetric response of the copper‑arsenic system at a mercury electrode

2020 ◽  
pp. 114833
Author(s):  
Jenny Gómez Avila ◽  
Jhan Carlos Burgos Gómez ◽  
Angélica C. Heredia ◽  
Mónica E. Crivello ◽  
Fernando Garay
Keyword(s):  
Mercury Electrode ◽  
Square Wave ◽  
Mechanistic Analysis ◽  
Copper Arsenic ◽  
Cathodic Stripping

HPTLC Separation of a Hepatoprotective Combination in Pharmaceutical Formulation and Human Plasma

2020 ◽  
Vol 58 (5) ◽  
pp. 411-417
Author(s):  
Maimana A Magdy ◽  
Rehab M Abdelfatah
Keyword(s):  
Human Plasma ◽  
High Performance ◽  
Limit Of Detection ◽  
Pharmaceutical Formulation ◽  
Limit Of Quantification ◽  
Ich Guidelines ◽  
Spiked Plasma ◽  
Significant Difference ◽  
Developing System

Abstract A binary mixture of Silymarin (SR) and Vitamin E (VE) acetate, of an antioxidant and a hepatoprotective effect, has been analyzed using a sensitive, selective and economic high performance thin layer chromatographic (HPTLC) method in their pure forms, pharmaceutical formulation and spiked human plasma. SR and VE were separated on 60F254 silica gel plates using hexane:acetone:formic acid (7:3:0.15, v/v/v) as a developing system with UV detection at 215 nm. The method was evaluated for linearity, accuracy, precision, selectivity, limit of detection (LOD) and limit of quantification (LOQ). SR and VE were detected in the linear range of 0.2–2.5 and 0.2–4.5 μg/band, respectively. Method validation was done as per ICH guidelines and acceptable results of accuracy of 99.86 ± 1.190 and 100.22 ± 1.609 for SR and VE, respectively were obtained. The method has been successfully applied for determination of the studied drugs in their pharmaceutical formulation without any interference from excipients, and in spiked plasma samples. Results obtained by the developed HPTLC-densitometric method were statistically compared to those obtained by the reported HPLC methods and no significant difference was found between them.

Three validated stripping voltammetric procedures for determination of the anti-prostate cancer drug flutamide in tablets and human serum at a mercury electrode

2004 ◽  
Vol 82 (9) ◽  
pp. 1386-1392 ◽  
Author(s):  
E Hammam ◽  
H S El-Desoky ◽  
K Y El-Baradie ◽  
A M Beltagi
Keyword(s):  
Prostate Cancer ◽  
Human Serum ◽  
Mercury Electrode ◽  
Stripping Voltammetry ◽  
Differential Pulse ◽  
Pharmaceutical Formulation ◽  
Cancer Drug ◽  
Linear Sweep ◽  
Square Wave

Flutamide is a nonsteriodal anti-androgen drug, which is commonly used in the treatment of advanced prostate cancer. Based on the reduction of the nitro organic moiety of the drug molecule in acetate buffer of pH 5 at the hanging mercury drop electrode, three adsorptive cathodic stripping voltammetric procedures were optimized for determination of flutamide in bulk, tablets, and human serum applying linear-sweep, differential-pulse, and square-wave waveforms. The achieved limits of detection of the bulk drug were 1.9 × 10–7, 8.7 × 10–8, and 9.7 × 10–9 mol L–1 by using the optimized differential-pulse, linear-sweep, and square-wave adsorptive stripping voltammetric procedures, respectively. Repeatability of the results was studied for 1 × 10–6 mol L–1 of the drug and the recoveries obtained were 98.51 ± 1.56% (LSV), 98.89 ± 0.87% (DPV), and 99.21 ± 1.03% (SWV). The proposed procedures were successfully applied to the determination of the drug in pharmaceutical formulation (Eulexin® tablets) and human serum. The detection limits of the drug in bulk, pharmaceutical formulation, and human serum achieved by means of the proposed procedures showed that the square-wave mode was more reliable for determination of the drug especially in its low concentration levels.Key words: flutamide, linear-sweep, differential-pulse, square-wave, cathodic adsorptive stripping voltammetry, determination, Eulexin® tablets, human serum.

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