scholarly journals Chondro-protective effects of low intensity pulsed ultrasound

2016 ◽  
Vol 24 (11) ◽  
pp. 1989-1998 ◽  
Author(s):  
S.M.Z. Uddin ◽  
B. Richbourgh ◽  
Y. Ding ◽  
A. Hettinghouse ◽  
D.E. Komatsu ◽  
...  
Keyword(s):  
Protective Effects ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals Protective effects of low-intensity pulsed ultrasound on mandibular condylar cartilage exposed to mechanical overloading

2018 ◽  
Author(s):  
Mutsumi Fujita ◽  
Minami Sato-Shigeta ◽  
Hiroki Mori ◽  
Akihiko Iwasa ◽  
Nobuhiko Kawai ◽  
...  
Keyword(s):  
Trabecular Bone ◽  
Mandibular Condyle ◽  
Protective Effects ◽  
Micro Ct ◽  
Pulsed Ultrasound ◽  
Condylar Cartilage ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Early Progression ◽  
Experimental Group

AbstractThe aim of this study was to examine the role of low-intensity pulsed ultrasound (LIPUS) exposure in the onset and early progression of temporomandibular joint (TMJ) osteoarthritis (TMJ-OA) induced by mechanical overloading. Fifteen-week-old male Wistar rats were divided into two experimental groups and a control group (n = 5 each). In the experimental groups, both TMJs were subjected to mechanical overloading by forced mouth opening using a jaw-opening device for 3 h/day for 5 continuous days. After mechanical overloading, TMJs in one experimental group were exposed to LIPUS for 20 min/day. After the experiments, mandibles were resected from the rats, and the condyles were processed. The bones were analyzed using high-resolution microcomputed tomography (micro-CT). The resected TMJs were also subjected to histological analysis and immunohistochemical staining. Micro-CT images of the mandibular condyle showed severe subchondral trabecular bone loss in the experimental group with overloading. Treatment with LIPUS after overloading resulted in decreased subchondral trabecular bone resorption. In TMJ sections from the experimental group with overloading, cell-free regions and proteoglycan loss characterized the cartilage degradation; LIPUS exposure restricted these changes in the mandibular condyle. Furthermore, the number of tartrate-resistant acid phosphatase-positive osteoclasts in the mineralized layer of the condylar cartilage increased after mechanical overloading and decreased after LIPUS treatment. Our findings suggest that LIPUS exposure after mechanical TMJ overloading downregulates subchondral trabecular bone resorption and proteoglycan loss in the mandibular condylar cartilage. Thus, it may prove to be protective effects of LIPUS exposure on onset and early progression of TMJ-OA induced by mechanical overloading.

scholarly journals Improved activity of MC3T3-E1 cells by the exciting piezoelectric BaTiO3/TC4 using low-intensity pulsed ultrasound

2021 ◽  
Vol 6 (11) ◽  
pp. 4073-4082
Author(s):  
Kunzhan Cai ◽  
Yilai Jiao ◽  
Quan Quan ◽  
Yulin Hao ◽  
Jie Liu ◽  
...  
Keyword(s):  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals Osteocytes as main responders to low-intensity pulsed ultrasound treatment during fracture healing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tatsuya Shimizu ◽  
Naomasa Fujita ◽  
Kiyomi Tsuji-Tamura ◽  
Yoshimasa Kitagawa ◽  
Toshiaki Fujisawa ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Transcriptional Control ◽  
Target Genes ◽  
Pulsed Ultrasound ◽  
Activated T Cells ◽  
In Vivo Models ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Ultrasound Stimulation

AbstractUltrasound stimulation is a type of mechanical stress, and low-intensity pulsed ultrasound (LIPUS) devices have been used clinically to promote fracture healing. However, it remains unclear which skeletal cells, in particular osteocytes or osteoblasts, primarily respond to LIPUS stimulation and how they contribute to fracture healing. To examine this, we utilized medaka, whose bone lacks osteocytes, and zebrafish, whose bone has osteocytes, as in vivo models. Fracture healing was accelerated by ultrasound stimulation in zebrafish, but not in medaka. To examine the molecular events induced by LIPUS stimulation in osteocytes, we performed RNA sequencing of a murine osteocytic cell line exposed to LIPUS. 179 genes reacted to LIPUS stimulation, and functional cluster analysis identified among them several molecular signatures related to immunity, secretion, and transcription. Notably, most of the isolated transcription-related genes were also modulated by LIPUS in vivo in zebrafish. However, expression levels of early growth response protein 1 and 2 (Egr1, 2), JunB, forkhead box Q1 (FoxQ1), and nuclear factor of activated T cells c1 (NFATc1) were not altered by LIPUS in medaka, suggesting that these genes are key transcriptional regulators of LIPUS-dependent fracture healing via osteocytes. We therefore show that bone-embedded osteocytes are necessary for LIPUS-induced promotion of fracture healing via transcriptional control of target genes, which presumably activates neighboring cells involved in fracture healing processes.

Low-intensity pulsed ultrasound affects RUNX2 immunopositive osteogenic cells in delayed clinical fracture healing

Bone ◽  
2009 ◽  
Vol 45 (5) ◽  
pp. 862-869 ◽  
Author(s):  
Sjoerd Rutten ◽  
Peter A. Nolte ◽  
Clara M. Korstjens ◽  
Jenneke Klein-Nulend
Keyword(s):  
Fracture Healing ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Osteogenic Cells ◽  
Low Intensity ◽  
Clinical Fracture

scholarly journals The effect of low intensity pulsed ultrasound on mandibular condylar growth in young adult rats

Bone Reports ◽  
2021 ◽  
pp. 101122
Author(s):  
Yasamin Hadaegh ◽  
Hasan Uludag ◽  
Douglas Dederich ◽  
Tarek H. El-Bialy
Keyword(s):  
Young Adult ◽  
Pulsed Ultrasound ◽  
Adult Rats ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity
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