scholarly journals Notochordal cell conditioned medium enhances the cartilage matrix production and reduces catabolism by human articular chondrocytes

2016 ◽  
Vol 24 ◽  
pp. S169
Author(s):  
S. Müller ◽  
L. Acevedo ◽  
M. Karim ◽  
A. Matta ◽  
A. Mehrkens ◽  
...  
Keyword(s):  
Conditioned Medium ◽  
Articular Chondrocytes ◽  
Cartilage Matrix ◽  
Human Articular Chondrocytes ◽  
Notochordal Cell ◽  
Matrix Production ◽  
Cell Conditioned Medium

scholarly journals The Species-Specific Regenerative Effect of Notochordal Cell-conditioned Medium on Degenerated Human Nucleus Pulposus Cells

2016 ◽  
Vol 6 (1_suppl) ◽  
pp. s-0036-1582597-s-0036-1582597
Author(s):  
Frances Bach ◽  
Stefan de Vries ◽  
Ferdi van Heel ◽  
Anita Krouwels ◽  
Laura Creemers ◽  
...  
Keyword(s):  
Conditioned Medium ◽  
Nucleus Pulposus ◽  
Nucleus Pulposus Cells ◽  
Notochordal Cell ◽  
Regenerative Effect ◽  
Species Specific ◽  
Cell Conditioned Medium

scholarly journals The Lysine Specific Demethylase-1 Negatively Regulates the COL9A1 Gene in Human Articular Chondrocytes

2020 ◽  
Vol 21 (17) ◽  
pp. 6322
Author(s):  
Anne-Laure Durand ◽  
Alexandre Dufour ◽  
Elisabeth Aubert-Foucher ◽  
Christine Oger-Desfeux ◽  
Marielle Pasdeloup ◽  
...  
Keyword(s):  
Articular Chondrocytes ◽  
Cartilage Matrix ◽  
Human Articular Chondrocytes ◽  
Collagen Molecule ◽  
Sequencing Analysis ◽  
Loss Of Function ◽  
Gene Encoding ◽  
Lysine Specific Demethylase ◽  
Function Strategy

Osteoarthritis (OA) is a degenerative disease of the joints which is associated with an impaired production of the cartilage matrix by the chondrocytes. Here, we investigated the role of Lysine-Specific Demethylase-1 (LSD1), a chromatin remodeling enzyme whose role in articular chondrocytes was previously associated with a catabolic activity and which is potentially involved during OA. Following a loss of function strategy and RNA sequencing analysis, we detail the genes which are targeted by LSD1 in human articular chondrocytes and identify COL9A1, a gene encoding the α1 chain of the cartilage-specific type IX collagen, as negatively regulated by LSD1. We show that LSD1 interacts with the transcription factor SOX9 and is recruited to the promoter of COL9A1. Interestingly, we observe that OA cartilage displays stronger LSD1 immunostaining compared with normal, and we demonstrate that the depletion of LSD1 in OA chondrocytes prevents the decrease in COL9A1 following Il-1β treatment. These results suggest LSD1 is a new regulator of the anabolic activity of articular chondrocytes potentially destabilizing the cartilage matrix, since it negatively regulates COL9A1, a gene encoding a crucial anchoring collagen molecule. This newly identified role played by LSD1 may thus participate in the alteration of the cartilage matrix during OA.

scholarly journals The Stimulatory Effect of Notochordal Cell-Conditioned Medium in a Nucleus Pulposus Explant Culture

2016 ◽  
Vol 22 (1-2) ◽  
pp. 103-110 ◽  
Author(s):  
Stefan A.H. de Vries ◽  
Marina van Doeselaar ◽  
Björn P. Meij ◽  
Marianna A. Tryfonidou ◽  
Keita Ito
Keyword(s):  
Conditioned Medium ◽  
Nucleus Pulposus ◽  
Explant Culture ◽  
Notochordal Cell ◽  
Cell Conditioned Medium

scholarly journals Stimulatory Effect of Notochordal Cell-Conditioned Medium in a Nucleus Pulposus Explant Culture

2015 ◽  
Vol 5 (1_suppl) ◽  
pp. s-0035-1554235-s-0035-1554235
Author(s):  
Stefan de Vries ◽  
Marina van Doeselaar ◽  
Björn Meij ◽  
Marianna Tryfonidou ◽  
Keita Ito
Keyword(s):  
Conditioned Medium ◽  
Nucleus Pulposus ◽  
Explant Culture ◽  
Notochordal Cell ◽  
Cell Conditioned Medium
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