scholarly journals Differences in the osteoarthritic synovial fluid composition and rheology between patients with or without flare-up. A pilot study

2012 ◽  
Vol 20 ◽  
pp. S244 ◽  
Author(s):  
T. Conrozier ◽  
J.-C. Balblanc ◽  
M. Piperno ◽  
M. Rinaudo
Keyword(s):  
Pilot Study ◽  
Synovial Fluid ◽  
Fluid Composition

scholarly journals Relationship of plasma and synovial fluid BMP-7 with disease severity in knee osteoarthritis patients: a pilot study

2009 ◽  
Vol 33 (4) ◽  
pp. 1171-1175 ◽  
Author(s):  
Sittisak Honsawek ◽  
Maneerat Chayanupatkul ◽  
Aree Tanavalee ◽  
Manoon Sakdinakiattikoon ◽  
Benjamad Deepaisarnsakul ◽  
...  
Keyword(s):  
Pilot Study ◽  
Synovial Fluid ◽  
Knee Osteoarthritis ◽  
Disease Severity ◽  
Relationship Of

Viability and cell death of synovial fluid neutrophils as diagnostic biomarkers in equine infectious joint disease: A pilot study

2012 ◽  
Vol 92 (1) ◽  
pp. 132-137 ◽  
Author(s):  
Jella Wauters ◽  
Ann Martens ◽  
Frederik Pille ◽  
Michèle Dumoulin ◽  
Frank Gasthuys ◽  
...  
Keyword(s):  
Cell Death ◽  
Pilot Study ◽  
Synovial Fluid ◽  
Joint Disease ◽  
Diagnostic Biomarkers

Synovial fluid β-endorphin level in avascular necrosis, rheumatoid arthritis, and osteoarthritis of the femoral head and knee. A controlled pilot study

2010 ◽  
Vol 30 (4) ◽  
pp. 537-540 ◽  
Author(s):  
Kálmán Toth ◽  
István Barna ◽  
György Nagy ◽  
Karoly Wellinger ◽  
Gyöngyi Horvath ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Pilot Study ◽  
Femoral Head ◽  
Synovial Fluid ◽  
Avascular Necrosis

scholarly journals Hyaluronic acid synthesis, degradation, and crosslinking in equine osteoarthritis: TNF-α-TSG-6-mediated HC-HA formation

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Diana C. Fasanello ◽  
Jin Su ◽  
Siyu Deng ◽  
Rose Yin ◽  
Marshall J. Colville ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hyaluronic Acid ◽  
Synovial Fluid ◽  
Complex Formation ◽  
Synovial Membrane ◽  
Fluid Composition ◽  
Fluid Viscosity ◽  
Tnf Α ◽  
Ha Synthase ◽  
And Cartilage

Abstract Background TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA). Here, we examined HA synthase and inflammatory gene expression; synovial fluid HA, TNF-α, and viscosity; and TSG-6-mediated HC-HA complex formation in an equine OA model. The objectives of this study were to (1) evaluate the TNF-α-TSG-6-HC-HA signaling pathway across multiple joint tissues, including synovial membrane, cartilage, and synovial fluid, and (2) determine the impact of OA on synovial fluid composition and biophysical properties. Methods HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage. HA molecular weight (MW) distributions were determined using agarose gel electrophoresis and solid-state nanopore measurements, and HC-HA complex formation was detected via immunoblotting and immunofluorescence. SEC-MALS was used to evaluate TSG-6-mediated HA crosslinking, and synovial fluid and HA solution viscosities were analyzed using multiple particle-tracking microrheology and microfluidic measurements, respectively. Results TNF-α concentrations were greater in OA synovial fluid, and TSG6 expression was upregulated in OA synovial membrane and cartilage. TSG-6-mediated HC-HA complex formation was greater in OA synovial fluid and tissues than controls, and HC-HA was localized to both synovial membrane and superficial zone chondrocytes in OA joints. SEC-MALS demonstrated macromolecular aggregation of low MW HA in the presence of TSG-6 and inter-α-inhibitor with concurrent increases in viscosity. Conclusions Synovial fluid TNF-α concentrations, synovial membrane and cartilage TSG6 gene expression, and HC-HA complex formation were increased in equine OA. Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking. The TNF-α-TSG-6-HC-HA pathway may represent a potential therapeutic target in OA.

Rheology of osteoarthritic synovial fluid mixed with viscosupplements: A pilot study

2011 ◽  
Vol 1 (4) ◽  
pp. 213-219 ◽  
Author(s):  
Petcharatana Bhuanantanondh ◽  
Dana Grecov ◽  
Ezra Kwok ◽  
Pierre Guy
Keyword(s):  
Pilot Study ◽  
Synovial Fluid

scholarly journals Characterization of endocannabinoids and related acylethanolamides in the synovial fluid of dogs with osteoarthritis: a pilot study

2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Carmela Valastro ◽  
Debora Campanile ◽  
Mariarosaria Marinaro ◽  
Delia Franchini ◽  
Fabiana Piscitelli ◽  
...  
Keyword(s):  
Pilot Study ◽  
Synovial Fluid

scholarly journals P116 ANALYSIS OF MOLECULAR WEIGHT OF HYALURONAN IN HUMAN SYNOVIAL FLUID AFTER GLUCOSAMINE TREATMENT: A PILOT STUDY

2006 ◽  
Vol 14 ◽  
pp. S74
Author(s):  
Y. Kanemoto ◽  
M. Watanabe ◽  
Y. Okada ◽  
S. Sugawara ◽  
Y. Nomura
Keyword(s):  
Molecular Weight ◽  
Pilot Study ◽  
Synovial Fluid ◽  
Human Synovial Fluid
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