scholarly journals Posttranscriptional Gene Regulation by Long Noncoding RNA

2013 ◽  
Vol 425 (19) ◽  
pp. 3723-3730 ◽  
Author(s):  
Je-Hyun Yoon ◽  
Kotb Abdelmohsen ◽  
Myriam Gorospe
Keyword(s):  
Gene Regulation ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Posttranscriptional Gene Regulation

Long noncoding RNA expression in dermal papilla cells contributes to hairy gene regulation

2014 ◽  
Vol 453 (3) ◽  
pp. 508-514 ◽  
Author(s):  
Chang-min Lin ◽  
Yang Liu ◽  
Keng Huang ◽  
Xian-cai Chen ◽  
Bo-zhi Cai ◽  
...  
Keyword(s):  
Gene Regulation ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Dermal Papilla ◽  
Rna Expression ◽  
Dermal Papilla Cells

scholarly journals The cardiac lncRNA Chantico directly regulates Cxcl1 chemokine transcription

2021 ◽  
Author(s):  
Johnathon M Hall ◽  
Amoldeep S Kainth ◽  
Megan J Rowton ◽  
Emery Lu ◽  
Gabriela Haddad ◽  
...  
Keyword(s):  
Gene Regulation ◽  
Transcription Factor ◽  
Nucleic Acid ◽  
Immune Cells ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Premature Termination ◽  
Chemokine Gene ◽  
Cis Acting

In local gene regulation, long noncoding RNA (lncRNA) loci can function at three distinct, but non-mutually exclusive levels: the RNA molecule itself, the process of its transcription, and/or the underlying DNA element. Yet for cis-acting lncRNA, these distinctions are particularly challenging with present tools. To address this problem, we developed Omegazymes, catalytic nucleic acid enzymes to specifically target lncRNA for degradation without triggering premature termination of their transcription. We use Omegazymes to selectively target one of a highly refined set of lncRNAs in the mouse cardiomyocytes, demonstrating that the Chantico lncRNA molecule directly potentiates the transcription of Cxcl1, a neighboring chemokine gene. We find that the Chantico locus also acts at the DNA-level, as the binding of an essential cardiac transcription factor to the Chantico promoter is necessary for Cxcl1 transcription in mature cardiomyocytes. This Chantico regulation of Cxcl1 impacts cardiomyocyte signaling to immune cells, potentially regulating tissue-residence and inflammation.

scholarly journals Long noncoding RNA and protein abundance in lncRNPs

RNA ◽  
2021 ◽  
pp. rna.078971.121
Author(s):  
Man Wu ◽  
Liang-Zhong Yang ◽  
Ling-Ling Chen
Keyword(s):  
Gene Regulation ◽  
Protein Expression ◽  
Relative Abundance ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Protein Complexes ◽  
Noncoding Rnas ◽  
Protein Abundance ◽  
Interacting Proteins ◽  
Low Levels

Although long noncoding RNAs (lncRNAs) are generally expressed at low levels, emerging evidence has revealed that many play important roles in gene regulation by a variety of mechanisms as they engage with proteins. Given that the abundance of proteins often greatly exceeds that of their interacting lncRNAs, quantification of the relative abundance, or even the exact stoichiometry in some cases, within lncRNA-protein complexes is helpful for understanding of the mechanism(s) of action of lncRNAs. We discuss methods used to examine lncRNA and protein expression at the single cell, sub-cellular and sub-organelle levels, the average and local lncRNA concentration in cells, as well as how lncRNAs can modulate the functions of their interacting proteins even at a low stoichiometric concentration.

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