scholarly journals Functional Versatility of a Single Protein Surface in Two Protein:Protein Interactions

2012 ◽  
Vol 419 (3-4) ◽  
pp. 223-233 ◽  
Author(s):  
Poorni R. Adikaram ◽  
Dorothy Beckett
Keyword(s):  
Protein Surface ◽  
Single Protein ◽  
Functional Versatility ◽  
Protein:Protein Interactions

Orientation of actin filaments during motion in motility assay

1995 ◽  
Vol 53 ◽  
pp. 52-53
Author(s):  
J. Borejdo ◽  
S. Burlacu
Keyword(s):  
Actin Filaments ◽  
Thin Filament ◽  
Striated Muscle ◽  
Myosin Head ◽  
Classical Method ◽  
Polarization Of Fluorescence ◽  
Single Protein ◽  
Intracellular Organelles ◽  
Change Orientation ◽  
Active Entity

Polarization of fluorescence is a classical method to assess orientation or mobility of macromolecules. It has been a common practice to measure polarization of fluorescence through a microscope to characterize orientation or mobility of intracellular organelles, for example anisotropic bands in striated muscle. Recently, we have extended this technique to characterize single protein molecules. The scientific question concerned the current problem in muscle motility: whether myosin heads or actin filaments change orientation during contraction. The classical view is that the force-generating step in muscle is caused by change in orientation of myosin head (subfragment-1 or SI) relative to the axis of thin filament. The molecular impeller which causes this change resides at the interface between actin and SI, but it is not clear whether only the myosin head or both SI and actin change orientation during contraction. Most studies assume that observed orientational change in myosin head is a reflection of the fact that myosin is an active entity and actin serves merely as a passive "rail" on which myosin moves.

1P285 Exhaustive search for protein surface similarities using a grid computing system

2005 ◽  
Vol 45 (supplement) ◽  
pp. S103
Author(s):  
R. Minai ◽  
M. Iwasaki ◽  
H. Murakmai ◽  
Y. Matsuo
Keyword(s):  
Grid Computing ◽  
Computing System ◽  
Exhaustive Search ◽  
Protein Surface
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