Mutational Specificities of Brominated DNA Adducts Catalyzed by Human DNA Polymerases

2011 ◽  
Vol 406 (5) ◽  
pp. 679-686 ◽  
Author(s):  
Akira Sassa ◽  
Toshihiro Ohta ◽  
Takehiko Nohmi ◽  
Masamitsu Honma ◽  
Manabu Yasui
Keyword(s):  
Dna Adducts ◽  
Dna Polymerases ◽  
Human Dna

Translesion Synthesis past Equine Estrogen-Derived 2‘-Deoxycytidine DNA Adducts by Human DNA Polymerases η and κ†

Biochemistry ◽  
2004 ◽  
Vol 43 (35) ◽  
pp. 11312-11320 ◽  
Author(s):  
Naomi Suzuki ◽  
Manabu Yasui ◽  
Y. R. Santosh Laxmi ◽  
Haruo Ohmori ◽  
Fumio Hanaoka ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Dna Polymerases ◽  
Translesion Synthesis ◽  
Human Dna

Translesion Synthesis Past Estrogen-Derived DNA Adducts by Human DNA Polymerases η and κ†

Biochemistry ◽  
2004 ◽  
Vol 43 (20) ◽  
pp. 6304-6311 ◽  
Author(s):  
Naomi Suzuki ◽  
Shinji Itoh ◽  
Kinning Poon ◽  
Chikahide Masutani ◽  
Fumio Hanaoka ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Dna Polymerases ◽  
Translesion Synthesis ◽  
Human Dna

scholarly journals Translesion Synthesis Past Tamoxifen-Derived DNA Adducts by Human DNA Polymerases η and κ†

Biochemistry ◽  
2006 ◽  
Vol 45 (39) ◽  
pp. 12167-12174 ◽  
Author(s):  
Manabu Yasui ◽  
Naomi Suzuki ◽  
Y. R. Santosh Laxmi ◽  
Shinya Shibutani
Keyword(s):  
Dna Adducts ◽  
Dna Polymerases ◽  
Translesion Synthesis ◽  
Human Dna

scholarly journals Translesion Synthesis acrossO6-Alkylguanine DNA Adducts by Recombinant Human DNA Polymerases

2006 ◽  
Vol 281 (50) ◽  
pp. 38244-38256 ◽  
Author(s):  
Jeong-Yun Choi ◽  
Goutam Chowdhury ◽  
Hong Zang ◽  
Karen C. Angel ◽  
Choua C. Vu ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Dna Polymerases ◽  
Translesion Synthesis ◽  
Human Dna

scholarly journals Translesion Synthesis Past Equine Estrogen-Derived 2‘-Deoxyadenosine DNA Adducts by Human DNA Polymerases η and κ†

Biochemistry ◽  
2006 ◽  
Vol 45 (19) ◽  
pp. 6187-6194 ◽  
Author(s):  
Manabu Yasui ◽  
Y. R. Santosh Laxmi ◽  
Sreenivasa R. Ananthoju ◽  
Naomi Suzuki ◽  
Sung Yeon Kim ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Dna Polymerases ◽  
Translesion Synthesis ◽  
Human Dna

Detection and characterization of DNA polymerase activity in Toxoplasma gondii

Parasitology ◽  
1993 ◽  
Vol 107 (2) ◽  
pp. 135-139 ◽  
Author(s):  
A. Makioka ◽  
B. Stavros ◽  
J. T. Ellis ◽  
A. M. Johnson
Keyword(s):  
Toxoplasma Gondii ◽  
Dna Polymerase ◽  
Dna Polymerases ◽  
Sedimentation Coefficient ◽  
Polymerase Activity ◽  
Magnesium Ions ◽  
Dna Polymerase Β ◽  
Human Dna ◽  
Approximate Molecular Weight

SUMMARYA DNA polymerase activity has been detected and characterized in crude extracts from tachzoites of Toxoplasma gondii. The enzyme has a sedimentation coefficient of 6·4 S, corresponding to an approximate molecular weight of 150000 assuming a globular shape. Like mammalian DNA polymerase α, the DNA polymerase of T. gondii was sensitive to N-ethylmaleimide and inhibited by high ionic strength. However, the enzyme activity was not inhibited by aphidicolin which is an inhibitor of mammalian DNA polymerases α, δ and ε and also cytosine-β-D-arabinofuranoside-5′-triphosphate which is an inhibitor of α polymerase. The activity was inhibited by 2′,3′-dideoxythymidine-5′-triphosphate which is an inhibitor of mammalian DNA polymerase β and γ. Magnesium ions (Mg2+) were absolutely required for activity and its optimal concentration was 6 mM. The optimum potassium (K+) concentration was 50 mM and a higher concentration of K+ markedly inhibited the activity. Activity was optimal at pH 8. Monoclonal antibodies against human DNA polymerase did not bind to DNA polymerase of T. gondii. Thus the T. gondii enzyme differs from the human enzymes and may be a useful target for the design of toxoplasmacidal drugs.

Enzymological Characterization of Human DNA Polymerases-α and β

1983 ◽  
pp. 17-55 ◽  
Author(s):  
David Korn ◽  
Paul A. Fisher ◽  
Teresa Shu-Fong Wang
Keyword(s):  
Dna Polymerases ◽  
Human Dna

scholarly journals DNA Polymerase: Structural Homology, Conformational Dynamics, and the Effects of Carcinogenic DNA Adducts

2010 ◽  
Vol 2010 ◽  
pp. 1-15 ◽  
Author(s):  
Richard G. Federley ◽  
Louis J. Romano
Keyword(s):  
Dna Replication ◽  
Dna Polymerase ◽  
Dna Adducts ◽  
Structural Changes ◽  
Dna Polymerases ◽  
Conformational Dynamics ◽  
Three Dimensional ◽  
Structural Homology ◽  
Human Right ◽  
Selection Of

DNA replication is vital for an organism to proliferate and lying at the heart of this process is the enzyme DNA polymerase. Most DNA polymerases have a similar three dimensional fold, akin to a human right hand, despite differences in sequence homology. This structural homology would predict a relatively unvarying mechanism for DNA synthesis yet various polymerases exhibit markedly different properties on similar substrates, indicative of each type of polymerase being prescribed to a specific role in DNA replication. Several key conformational steps, discrete states, and structural moieties have been identified that contribute to the array of properties the polymerases exhibit. The ability of carcinogenic adducts to interfere with conformational processes by directly interacting with the protein explicates the mutagenic consequences these adducts impose. Recent studies have identified novel states that have been hypothesised to test the fit of the nascent base pair, and have also shown the enzyme to possess a lively quality by continually sampling various conformations. This review focuses on the homologous structural changes that take place in various DNA polymerases, both replicative and those involved in adduct bypass, the role these changes play in selection of a correct substrate, and how the presence of bulky carcinogenic adducts affects these changes.

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