scholarly journals Involvement of DEAD-box Proteins in Group I and Group II Intron Splicing. Biochemical Characterization of Mss116p, ATP Hydrolysis-dependent and -independent Mechanisms, and General RNA Chaperone Activity

2007 ◽  
Vol 365 (3) ◽  
pp. 835-855 ◽  
Author(s):  
Coralie Halls ◽  
Sabine Mohr ◽  
Mark Del Campo ◽  
Quansheng Yang ◽  
Eckhard Jankowsky ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Atp Hydrolysis ◽  
Group Ii Intron ◽  
Chaperone Activity ◽  
Intron Splicing ◽  
Rna Chaperone ◽  
Dead Box ◽  
Group I ◽  
Group Ii

scholarly journals A DEAD-box protein alone promotes group II intron splicing and reverse splicing by acting as an RNA chaperone

2006 ◽  
Vol 103 (10) ◽  
pp. 3569-3574 ◽  
Author(s):  
S. Mohr ◽  
M. Matsuura ◽  
P. S. Perlman ◽  
A. M. Lambowitz
Keyword(s):  
Group Ii Intron ◽  
Intron Splicing ◽  
Rna Chaperone ◽  
Dead Box ◽  
Group Ii

scholarly journals ATP-Dependent Roles of the DEAD-Box Protein Mss116p in Group II Intron Splicing In Vitro and In Vivo

2011 ◽  
Vol 411 (3) ◽  
pp. 661-679 ◽  
Author(s):  
Jeffrey P. Potratz ◽  
Mark Del Campo ◽  
Rachel Z. Wolf ◽  
Alan M. Lambowitz ◽  
Rick Russell
Keyword(s):  
Group Ii Intron ◽  
Intron Splicing ◽  
Dead Box ◽  
The Dead ◽  
Group Ii

scholarly journals ATP utilization by a DEAD-box protein during refolding of a misfolded group I intron ribozyme

2020 ◽  
pp. jbc.RA120.015029
Author(s):  
Inga Jarmoskaite ◽  
Pilar Tijerina ◽  
Rick Russell
Keyword(s):  
Neurospora Crassa ◽  
Atp Hydrolysis ◽  
Group I Intron ◽  
Group I Introns ◽  
Structural Rearrangements ◽  
Rna Chaperone ◽  
Dead Box ◽  
Group I ◽  
Rna Biology ◽  
Evolutionary Convergence

DEAD-box helicase proteins perform ATP-dependent rearrangements of structured RNAs throughout RNA biology. Short RNA helices are unwound in a single ATPase cycle, but the ATP requirement for more complex RNA structural rearrangements is unknown. Here we measure the amount of ATP used for native refolding of a misfolded group I intron ribozyme by CYT-19, a Neurospora crassa DEAD-box protein that functions as a general chaperone for mitochondrial group I introns. By comparing the rates of ATP hydrolysis and ribozyme refolding, we find that several hundred ATP molecules are hydrolyzed during refolding of each ribozyme molecule. After subtracting non-productive ATP hydrolysis that occurs in the absence of ribozyme refolding, we find that approximately 100 ATPs are hydrolyzed per refolded RNA as a consequence of interactions specific to the misfolded ribozyme. This value is insensitive to changes in ATP and CYT-19 concentration and decreases with decreasing ribozyme stability. Because of earlier findings that ~90% of global ribozyme unfolding cycles lead back to the kinetically preferred misfolded conformation and are not observed, we estimate that each global unfolding cycle consumes ~10 ATPs. Our results indicate that CYT-19 functions as a general RNA chaperone by using a stochastic, energy-intensive mechanism to promote RNA unfolding and refolding, suggesting an evolutionary convergence with protein chaperones.

scholarly journals A DEAD-Box Protein Functions as an ATP-Dependent RNA Chaperone in Group I Intron Splicing

Cell ◽  
2002 ◽  
Vol 109 (6) ◽  
pp. 769-779 ◽  
Author(s):  
Sabine Mohr ◽  
John M. Stryker ◽  
Alan M. Lambowitz
Keyword(s):  
Group I Intron ◽  
Intron Splicing ◽  
Rna Chaperone ◽  
Dead Box ◽  
Group I ◽  
Protein Functions

The DEAD-box protein PMH2 is required for efficient group II intron splicing in mitochondria of Arabidopsis thaliana

2009 ◽  
Vol 72 (4-5) ◽  
pp. 459-467 ◽  
Author(s):  
Daniela Köhler ◽  
Stephanie Schmidt-Gattung ◽  
Stefan Binder
Keyword(s):  
Arabidopsis Thaliana ◽  
Group Ii Intron ◽  
Intron Splicing ◽  
Dead Box ◽  
The Dead ◽  
Group Ii

scholarly journals The splicing of yeast mitochondrial group I and group II introns requires a DEAD-box protein with RNA chaperone function

2004 ◽  
Vol 102 (1) ◽  
pp. 163-168 ◽  
Author(s):  
H.-R. Huang ◽  
C. E. Rowe ◽  
S. Mohr ◽  
Y. Jiang ◽  
A. M. Lambowitz ◽  
...  
Keyword(s):  
Group Ii Introns ◽  
Rna Chaperone ◽  
Chaperone Function ◽  
Dead Box ◽  
Group I ◽  
Group Ii

scholarly journals Do DEAD-Box Proteins Promote Group II Intron Splicing without Unwinding RNA?

2007 ◽  
Vol 28 (1) ◽  
pp. 159-166 ◽  
Author(s):  
Mark Del Campo ◽  
Pilar Tijerina ◽  
Hari Bhaskaran ◽  
Sabine Mohr ◽  
Quansheng Yang ◽  
...  
Keyword(s):  
Group Ii Intron ◽  
Intron Splicing ◽  
Dead Box ◽  
Group Ii
Sign in / Sign up

Export Citation Format

Share Document