scholarly journals Molecular Characterization of Carbapenem Resistant Enterobacteriaceae in Tertiary Care Center

2020 ◽  
Vol 13 (2) ◽  
pp. 318-319
Author(s):  
S. Alyami ◽  
R. Cruzz ◽  
S. Aljohani
Keyword(s):  
Molecular Characterization ◽  
Care Center ◽  
Tertiary Care ◽  
Tertiary Care Center ◽  
Carbapenem Resistant ◽  
Carbapenem Resistant Enterobacteriaceae

Clinical and molecular characterization of children with neonatal diabetes mellitus at a tertiary care center in northern India

2017 ◽  
Vol 54 (6) ◽  
pp. 467-471 ◽  
Author(s):  
Vandana Jain ◽  
Amit Satapathy ◽  
Jaivinder Yadav ◽  
Rajni Sharma ◽  
Venkatesan Radha ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Molecular Characterization ◽  
Care Center ◽  
Tertiary Care ◽  
Neonatal Diabetes ◽  
Neonatal Diabetes Mellitus ◽  
Tertiary Care Center ◽  
Northern India

scholarly journals Prevalence of carbapenem resistance genes and corresponding MIC90 in Enterobacteriaceae at a tertiary care center in Lebanon

2018 ◽  
Vol 12 (02.1) ◽  
pp. 9S
Author(s):  
Kohar Annie Kissoyan ◽  
George F Araj ◽  
Ghassan M Matar
Keyword(s):  
Care Center ◽  
Outer Membrane Proteins ◽  
Tertiary Care ◽  
Carbapenem Resistance ◽  
Tertiary Care Center ◽  
E Coli ◽  
Carbapenem Resistant ◽  
Encoding Gene ◽  
Encoding Genes ◽  
Carbapenem Resistant Enterobacteriaceae

Introduction: The aim of this study was to correlate genes involved in carbapenem resistance to MIC levels among clinical ESBL and non-ESBL producing carbapenem resistant Enterobacteriaceae (CRE) isolates of Escherichia coli and Klebsiella pneumoniae. Methodology: E. coli (n = 76) and K. pneumoniae (n = 54), collected between July 2008 and July 2014, were analyzed. The MICs were determined against ertapenem (ERT), imipenem (IMP) and meropenem (MER). PCR was performed on all 130 isolates to amplify the resistance and outer membrane proteins (OMPs) encoding genes: blaOXA-48, blaNDM-1, blaTEM-1, blaCTX-M-15, ompC and ompF. Sequencing was performed on selected isolates. Results: The prevalence of blaOXA-48, blaNDM-1, blaTEM-1, and/or blaCTX-M-15 among E. coli isolates were 36%, 12%, 20% and 80%, respectively, while among K. pneumoniae they were 37%, 28%, 28% and 72%, respectively. K. pneumoniae isolates positive for any of these genes had an MIC90 > 32μg/mL against ERT, IMP and MER, while in E. coli isolates there was a variation in the MIC90 values. Porin impermeabilities were due to mutations in ompC and ompF genes in E. coli, and loss of ompC and ompF genes in K. pneumoniae, and increased MIC90 values. Conclusion: The presence of more than one carbapenem resistance encoding gene and/or ESBL encoding gene did not have an effect on the MIC90 value in K. pneumoniae isolates, while in E. coli it showed higher MIC90 values.

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