scholarly journals In-vitro evaluation of the antibacterial activity of the essential oils of Micromeria barbata, Eucalyptus globulus and Juniperus excelsa against strains of Mycobacterium tuberculosis (including MDR), Mycobacterium kansasii and Mycobacterium gordonae

2019 ◽  
Vol 12 (5) ◽  
pp. 615-618 ◽  
Author(s):  
Khaled El Omari ◽  
Monzer Hamze ◽  
Saer Alwan ◽  
Marwan Osman ◽  
Charafeddine Jama ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Mycobacterium Tuberculosis ◽  
Essential Oils ◽  
Eucalyptus Globulus ◽  
Mycobacterium Kansasii ◽  
In Vitro Evaluation ◽  
Juniperus Excelsa

In Vitro Evaluation of the Antibacterial Activity and Antibiotic- Modulatory Effect of Gracilaria cervicornis (Tuner) J. Agardh

2018 ◽  
Vol 15 (3) ◽  
Author(s):  
Israeliane F. Cordeiro ◽  
Henrique D. M. Coutinho ◽  
Joao V. A. Ferreira ◽  
Lucindo J. Quintans Junior ◽  
Irwin R. A. Menezes ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
In Vitro Evaluation

scholarly journals Evaluation of the in vitro antibacterial activity of some essential oils and their blends against Staphylococcus spp. isolated from episodes of sheep mastitis

2021 ◽  
Author(s):  
Filippo Fratini ◽  
Margherita Giusti ◽  
Simone Mancini ◽  
Francesca Pisseri ◽  
Basma Najar ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Diffusion Method ◽  
Minimal Bactericidal Concentration ◽  
Coagulase Negative Staphylococci ◽  
Disk Diffusion Method ◽  
In Vitro Antibacterial Activity ◽  
High Concentrations ◽  
Thymus Serpyllum

AbstractStaphylococcus aureus and coagulase-negative staphylococci are among the major causes of mastitis in sheep. The main goal of this research was to determine the in vitro antibacterial activity of several essential oils (EOs, n 30), then five of them were chosen and tested alone and in blends against staphylococci isolates. Five bacteria were isolated from episodes of ovine mastitis (two S. aureus and three S. xylosus). Biochemical and molecular methods were employed to identify the isolates and disk diffusion method was performed to determine their antimicrobial-resistance profile. The relative percentage of the main constituents in the tested essential oils and their blends was detected by GC-EIMS analysis. Antibacterial and bactericidal effectiveness of essential oils and blends were evaluated through minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). All of them showed sensitivity to the used antimicrobials. The EOs with the highest antibacterial activity were those belonging to the Lamiaceae family characterized by high concentrations of thymol, carvacrol and its precursor p-cymene, together with cinnamon EO, rich in cinnamaldehyde. In terms of both MIC and MBC values, the blend composed by Thymus capitatus EO 40%, Cinnamomum zeylanicum EO 20%, Thymus serpyllum EO 20% and Satureja montana EO 20% was found to be the most effective against all the isolates. Some essential oils appear to represent, at least in vitro, a valid tool against ovine mastitis pathogens. Some blends showed a remarkable effectiveness than the single oils, highlighting a synergistic effect in relation to the phytocomplex.

scholarly journals Analisa Aktivitas Antibakteri Rebusan Daun Sirih Dengan Rebusan Daun Kemangi Terhadap Pertumbuhan Bakteri Streptococcus mutans

2020 ◽  
Vol 18 (1) ◽  
pp. 36
Author(s):  
ENNY WILLIANTI ◽  
THEODORA THEODORA ◽  
WAHYUNI DYAH PARMASARI
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Streptococcus Mutans ◽  
Observational Research ◽  
Ocimum Sanctum ◽  
Piper Betle ◽  
Oral Flora ◽  
Betel Leaf ◽  
Growth Of Bacteria

<p><strong>ABSTRACT</strong><strong></strong></p><p><strong> </strong></p><p><strong>Background</strong>: Betel leaf contains essential oils consisting of bethelphenol, kavikol, sesquiterpenes, hydroxycavikol, cavibetol, estragol, eugenol and carvacrol. Essential oils are antibacterial due to the presence of phenol compounds and their derivatives that can denature the bacterial cell proteins. Basil leaves contain compounds from essential oils, namely 1,8-cineole, ß-bisabolene, and methyl eugenol. These three ingredients are soluble to ethanol and can cause damage to the cell membranes of the Streptococcus mutans bacteria, which are members of the normal oral flora but can turn into pathogens if the balance of normal flora is disturbed. The aim of this study was to determine the difference in the activity of the antibacterial  of decoction betel leaf (piper betle L. ) with a decoction of basil leaves (ocimum sanctum) against growth of bacteria <em>Streptococcus mutans</em> (in vitro study).</p><p><strong>M</strong><strong>ethod:</strong> this observational research with disk diffusion techniques. This study observed and measured the diameter of the inhibitory zone in MHA formed by decoction of betel leaf (piper betle L) and basil leaf (ocimum sanctum) in units of millimeters (mm). There were 2 groups with 16 replications.</p><p><strong>R</strong><strong>esults</strong>: the results of the description test showed that the antibacterial activity of the betel leaf decoction and the highest decoction of basil leaf was 17 mm and the lowest was 15 mm, but the average antibacterial value of betel leaf decoction (15,81) greater than the average value of antibacterial activity of basil leaf (15.75). This is because there are chemicals contained in betel leaf similar as contained in basil leaf, namely essential oils.</p><p><strong>Conclusion</strong>: there is no difference in the antibacterial activity of decoction  betel leaf with decoction basil leaf against growth of bacteria <em>Streptococcus mutans</em>.</p><p><strong> </strong></p><p><strong>Keywords</strong>: Betel leaf decoction, basil leaf  decoction, Streptococcus <strong>mutans.      </strong></p><p><strong> </strong></p><p><strong> </strong></p><p><strong>Abstrak</strong><strong></strong></p><p><strong> </strong></p><p><strong>Latar Belakang</strong>: Daun sirih mengandung minyak atsiri yang terdiri dari <em>bethelphenol, kavikol, </em>seskuiterpen, hydroxycavikol,cavibetol, estragol, eugenol dan carvacrol. Minyak atsiri bersifat antibakteri karena adanya senyawa phenol dan turunannya yang dapat mendenaturasi protein sel bakteri. Daun kemangi mengandung senyawa dari minyak atsiri yaitu <em>1,8-cineole</em>, <em>ß-bisabolene</em>, <em>metyl eugenol</em>. Ketiga bahan tersebut memiliki sifat larut terhadap etanol dan dapat menyebabkan kerusakan membran sel bakteri <em>streptococcus mutans</em> yang merupakan anggota flora normal rongga mulut tetapi dapat berubah menjadi patogen jika keseimbangan flora normal terganggu.Tujuan penelitian ini untuk mengetahui perbedaan aktivitas antibakteri rebusan daun sirih (<em>piper betle</em> L) dengan rebusan daun kemangi (<em>ocimum sanctum</em>) terhadap pertumbuhan bakteri <em>Streptococcus mutans</em> (penelitian in vitro).</p><p><strong>Metode</strong>: penelitian observasional ini dengan teknik difusi. Penelitian ini dilakukan dengan mengamati dan mengukur diameter zona hambat pada MHA yang dibentuk oleh rebusan daun sirih (<em>piper betle</em> L) dan daun kemangi (<em>ocimum sanctum</em>) dalam satuan milimeter (mm). Terdapat 2 kelompok dengan replikasi sebanyak 16.</p><p><strong>Hasil</strong> : Hasil uji deskripsi menunjukkan bahwa aktivitas antibakteri pada rebusan daun sirih maupun rebusan daun kemangi yang tertinggi sebesar 17 mm dan yang terendah 15 mm. Tetapi pada nilai rata-rata efektifitas antibakteri rebusan daun sirih (15,81) lebih besar daripada nilai rata-rata efektifitas antibakteri rebusan daun kemangi (15,75). Hal ini dikarenakan ada zat kimia yang terkandung dalam daun sirih mirip dengan yang terkandung dalam daun kemangi, yaitu minyak atsiri.</p><p><strong>Kesimpulan</strong> : tidak ada perbedaan aktivitas antibakteri rebusan daun sirih dengan rebusan daun kemangi terhadap pertumbuhan bakteri <em>Streptococcus </em><em>m</em><em>utans</em>.</p><p><strong> </strong></p><p><strong>Kata kunci</strong>:  rebusan daun sirih, rebusan daun kemangi<em>, Streptococcus mutans</em>.</p><p> </p><p>     </p>

scholarly journals Anti-bacterial activity of three essential oils - An in vitro study

2019 ◽  
Vol 10 (2) ◽  
pp. 1049-1053 ◽  
Author(s):  
Geetha RV ◽  
John Rozar Raj B ◽  
Lakshmi Thangavelu
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Streptococcus Mutans ◽  
Bacterial Species ◽  
In Vitro Study ◽  
Oral Bacteria ◽  
Zone Of Inhibition ◽  
Thyme Oil ◽  
Rosemary Oil

To conduct a study regarding the antibacterial activity of essential oils against bacteria causing Caries. Essential oils are distillates of the volatile compounds of a plant’s secondary metabolism and may act as photoprotective agents. Their curative effect has been known since antiquity. It is based on a variety of pharmacological properties which are specific for each plant species. The mouth contains a variety of oral bacteria, but only a few species of bacteria are believed to cause dental caries. Antibacterial activity of the three essential oils, Rosemary oil, Holy basil oil, Thyme oil was screened against Streptococcus mutans, using disc diffusion technique. The rosemary oil was more effective against Streptococcus mutans with a zone of inhibition of 52 mm diameter (at concentration 200 µl), Rosemary oil showed a zone of inhibition of 44 mm diameter and with thyme oil, the zone diameter was 30 mm. The results of this study showed that the essential oils at different concentrations exhibited antibacterial activity against the bacterial species tested.

scholarly journals Chemical Composition, Antioxidant and Antibacterial Activity of Bunium persicum, Eucalyptus globulus, and Rose Water on Multidrug-Resistant Listeria Species

2018 ◽  
Vol 23 ◽  
pp. 2515690X1775131 ◽  
Author(s):  
Farhad Sharafati Chaleshtori ◽  
Mohamad Saholi ◽  
Reza Sharafati Chaleshtori
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Eucalyptus Globulus ◽  
Multidrug Resistant ◽  
Bacterial Strains ◽  
Antioxidant Power ◽  
Listeria Species ◽  
Bunium Persicum

This research was aimed at investigating the antioxidant and antibacterial activity of Bunium persicum, Eucalyptus globulus, and rose water on multidrug-resistant Listeria species. The antibiotic resistance of Listeria spp obtained from seafood samples were determined by the Kirby-Bauer method. The antioxidant and antibacterial activity of the essential oils and extracts were evaluated using ferric reducing antioxidant power and microdilution methods, respectively. A total 2 samples (1.88%) were positive for Listeria spp. L monocytogenes was found to be resistant to ampicillin, amoxicillin/clavulanic acid, penicillin, vancomycin, and kanamycin. B persicum essential oil showed the greatest antioxidant activity (248.56 ± 1.09 µM Fe2+/g). The E globulus essential oil showed consistently strong antimicrobial activity against L monocytogenes and L grayi, while rose water showed no antimicrobial activity against any of the tested bacterial strains. The results showed that after adding the B persicum and E globulus essential oils to bacteria, the cell components’ release increased significantly.

scholarly journals CHEMICAL COMPOSITION OF ESSENTIAL OILS FROM VITEX AGNUS-CASTUS L. GROWING IN MOROCCO AND ITS IN VITRO ANTIBACTERIAL ACTIVITY AGAINST CLINICAL BACTERIA RESPONSIBLE FOR NOSOCOMIAL INFECTIONS

2018 ◽  
Vol 11 (10) ◽  
pp. 365 ◽  
Author(s):  
Fatima El Kamari ◽  
Amal Taroq ◽  
Yassine El Atki ◽  
Imane Aouam ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Nosocomial Infections ◽  
University Hospital ◽  
Mass Spectrometry Analysis ◽  
Diffusion Method ◽  
Strong Activity ◽  
Agnus Castus

Objective: The aim of the current study is to determine the chemical composition and evaluate antibacterial activity of Vitex agnus-castus L. (VAC) essential oils against some bacteria causing nosocomial infections in the neonatal and intensive care rooms at the university hospital center of Fez Morocco. Methods: The phytochemical screening of essential oils was determined using gas chromatography (GC) and GC-mass spectrometry analysis. The antibacterial test was evaluated against Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria species (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Proteus mirabilis) using disc diffusion method. Results: Twenty-nine components were identified in the fruits’ oil representing 93.1% of total oil. The major components in the fruits oil are 1,8-cineole (11.6%), α-thujene (9.3%), phyllocladene (8.2%), α-pinene (7.9%), caryophyllene (5.9%), and cubenol (5%). Furthermore, 28 components were identified in the leaf essential oil. The main component was caryophyllene (9.5%), followed by 1,8-cineole (8.7%), manoyl oxide (7.3%), eugenyl acetate (7.1%), phyllocladene (6.8%), and α-pinene (5.2%). Antibacterial activity of both oils showed a strong activity against nosocomial bacteria tested. Conclusion: Essential oils of Moroccan VAC could be exploited as natural drugs for bacteria, especially those who have acquired resistance to conventional antibiotics.

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