scholarly journals Molecular characterization of antimicrobial resistance genes against Staphylococcus aureus isolates from Trinidad and Tobago

2017 ◽  
Vol 10 (3) ◽  
pp. 316-323 ◽  
Author(s):  
Patrick E. Akpaka ◽  
Rashida Roberts ◽  
Stefan Monecke
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Trinidad And Tobago ◽  
Molecular Characterization ◽  
Resistance Genes ◽  
Antimicrobial Resistance Genes

scholarly journals Molecular characterization of multidrug-resistant Shiga toxin-producing Escherichia coli harboring antimicrobial resistance genes obtained from a farmhouse

2019 ◽  
Vol 113 (6) ◽  
pp. 268-274 ◽  
Author(s):  
João Pedro Rueda Furlan ◽  
Inara Fernanda Lage Gallo ◽  
Anna Carolina Leonelli Pires de Campos ◽  
Jaqueline Passaglia ◽  
Juliana Pfrimer Falcão ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Resistance Genes ◽  
Shiga Toxin ◽  
Multidrug Resistant ◽  
Antimicrobial Resistance Genes

scholarly journals Culture-Independent Genotyping, Virulence and Antimicrobial Resistance Gene Identification of Staphylococcus aureus from Orthopaedic Implant-Associated Infections

2021 ◽  
Vol 9 (4) ◽  
pp. 707
Author(s):  
J. Christopher Noone ◽  
Fabienne Antunes Ferreira ◽  
Hege Vangstein Aamot
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Virulence Gene ◽  
Orthopaedic Implant ◽  
Metagenomic Sequencing ◽  
Gene Detection ◽  
Shotgun Metagenomic Sequencing ◽  
Antimicrobial Resistance Genes ◽  
Culture Independent

Our culture-independent nanopore shotgun metagenomic sequencing protocol on biopsies has the potential for same-day diagnostics of orthopaedic implant-associated infections (OIAI). As OIAI are frequently caused by Staphylococcus aureus, we included S. aureus genotyping and virulence gene detection to exploit the protocol to its fullest. The aim was to evaluate S. aureus genotyping, virulence and antimicrobial resistance genes detection using the shotgun metagenomic sequencing protocol. This proof of concept study included six patients with S. aureus-associated OIAI at Akershus University Hospital, Norway. Five tissue biopsies from each patient were divided in two: (1) conventional microbiological diagnostics and genotyping, and whole genome sequencing (WGS) of S. aureus isolates; (2) shotgun metagenomic sequencing of DNA from the biopsies. Consensus sequences were analysed using spaTyper, MLST, VirulenceFinder, and ResFinder from the Center for Genomic Epidemiology (CGE). MLST was also compared using krocus. All spa-types, one CGE and four krocus MLST results matched Sanger sequencing results. Virulence gene detection matched between WGS and shotgun metagenomic sequencing. ResFinder results corresponded to resistance phenotype. S. aureus spa-typing, and identification of virulence and antimicrobial resistance genes are possible using our shotgun metagenomics protocol. MLST requires further optimization. The protocol has potential application to other species and infection types.

Characterization of antimicrobial resistance genes in Enterobacteriaceae carried by suburban mesocarnivores and locally owned and stray dogs

2020 ◽  
Vol 67 (4) ◽  
pp. 460-466
Author(s):  
Katherine E. L. Worsley‐Tonks ◽  
Elizabeth A. Miller ◽  
Stanley D. Gehrt ◽  
Shane C. McKenzie ◽  
Dominic A. Travis ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Stray Dogs ◽  
Antimicrobial Resistance Genes
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