scholarly journals Isolation and characterization of Listeria monocytogenes and other Listeria species in foods of animal origin in Addis Ababa, Ethiopia

2011 ◽  
Vol 4 (1) ◽  
pp. 22-29 ◽  
Author(s):  
Simon Gebretsadik ◽  
Tesfu Kassa ◽  
Haile Alemayehu ◽  
Kahsay Huruy ◽  
Nigatu Kebede
Keyword(s):  
Listeria Monocytogenes ◽  
Addis Ababa ◽  
Animal Origin ◽  
Listeria Species ◽  
Isolation And Characterization

0602 Isolation and characterization of listeriaphages for control of growth of Listeria monocytogenes in dairy foods

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 285-286
Author(s):  
S. H. Lee ◽  
H. S. Lee ◽  
S. Heo ◽  
C. R. Lee ◽  
G. B. Kim
Keyword(s):  
Listeria Monocytogenes ◽  
Dairy Foods ◽  
Isolation And Characterization

Isolation and characterization of Listeria monocytogenes from commercial asazuke (Japanese light pickles)

2010 ◽  
Vol 139 (3) ◽  
pp. 134-139 ◽  
Author(s):  
Khuanwalai Maklon ◽  
Atsuka Minami ◽  
Akiko Kusumoto ◽  
Koichi Takeshi ◽  
Nguyen Thi Bich Thuy ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Isolation And Characterization

Isolation and characterization of Listeria monocytogenes in Chinese food obtained from the central area of China

Food Control ◽  
2017 ◽  
Vol 74 ◽  
pp. 9-16 ◽  
Author(s):  
Xin-jun Du ◽  
Xiang Zhang ◽  
Xiao-yi Wang ◽  
Yu-lan Su ◽  
Ping Li ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Central Area ◽  
Isolation And Characterization ◽  
Chinese Food

Isolation and characterization of Listeria species from tropical seafoods

Food Control ◽  
2008 ◽  
Vol 19 (6) ◽  
pp. 566-569 ◽  
Author(s):  
V.S. Parihar ◽  
S.B. Barbuddhe ◽  
M.L. Danielsson-Tham ◽  
W. Tham
Keyword(s):  
Listeria Species ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Coronavirus and Rotavirus Associated With Calves, in Central Part of Oromia, Ethiopia

2020 ◽  
Author(s):  
Umer Seid Geletu ◽  
Fufa Dawo Bari ◽  
Munera Ahmednur Usmael ◽  
Asamino Tesfaye
Keyword(s):  
Diarrheal Disease ◽  
Addis Ababa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infection Prevalence ◽  
Rt Pcr ◽  
Cross Sectional ◽  
Fecal Samples ◽  
Rotavirus Infection ◽  
Isolation And Characterization

Abstract Background: Coronavirus and Rotavirus are most commonly associated etiologies for calves’ diarrhea resulting in loss of productivity and economy of farmers. However, various facets of diarrheal disease caused by coronavirus and rotavirus in calves in Ethiopia are inadequately understood. A cross sectional study was conducted with the aim of isolation and molecular characterization of coronavirus and rotavirus from calves in central part of Oromia (Bishoftu, Sebata, Holeta and Addis Ababa), Ethiopia from November 2018 to May 2019. The four study areas were purposively selected and fecal samples were collected by simple random sampling for diagnosis of coronavirus and rotavirus infection by using antigen detection Enzyme linked immunosorbent assay (Ag-ELISA) kit. In addition, this study was carried out to have insight in prevalence and associated risk factors of coronavirus and rotavirus infection in calves. Result: During the study 83 diarrheic and 162 non-diarrheic fecal samples collected from calves less than 4 weeks of age were screened for coronavirus and rotavirus. Of the 83 diarrheic samples, 1 sample (1.2%) was positive for coronavirus antigen (Ag) and 6 samples (7.2%) were found to be positive for rotavirus antigen (Ag) by Ag-ELISA. All the non-diarrheic samples were negative for both coronavirus and rotavirus Ag. The overall prevalence of coronavirus and rotavirus infection in calves were estimated as 0.4% (1/245) and 2.45% (6/245) respectively. All samples (7) of ELISA test positive of both coronavirus and rotavirus were propagated in Madin Darby bovine kidney cells (MDBK). After 3 subsequent passages, progressive cytopathic effect (CPE) i.e. rounding, detachment as well as destruction of mono-layer cell of five sample (1 sample of coronavirus and 4 sample of rotavirus) (71.4%) were observed. At the molecular stage, reverse transcriptase polymerase chain reaction (RT-PCR) technique was used to determine the presence of coronavirus and rotavirus nucleic acid by using specific primers. The 5 samples that were coronavirus and rotavirus antigen positive by ELISA and develop CPE on cell culture were also positive on RT-PCR technique. Infection prevalence peaked have been obtained at 1st and 2nd weeks of age in male calves. Conclusion: Diarrheal disease caused by coronavirus and rotavirus has a great health problem in calves that interrupts production benefits with reduced weight gain and increased mortality, and its potential for zoonotic spread. So the present findings show coronavirus and rotavirus infection in calves in Ethiopia that needs to be addressed by practicing early colostrums feeding in newborn calves, using vaccine, or improving livestock management.

scholarly journals Isolation and Characterization of Clinical Listeria monocytogenes in Beijing, China, 2014–2016

2019 ◽  
Vol 10 ◽  
Author(s):  
Xiaoai Zhang ◽  
Yanlin Niu ◽  
Yuzhu Liu ◽  
Zheng Lu ◽  
Di Wang ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Isolation And Characterization ◽  
Beijing China
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