Evaluation of different culture media for the isolation and growth of the fastidious Vibrio tapetis, the causative agent of brown ring disease

2012 ◽  
Vol 111 (1) ◽  
pp. 74-81 ◽  
Author(s):  
Sabela Balboa ◽  
Ana L. Dieguez ◽  
Alejandra Doce ◽  
Juan L. Barja ◽  
Jesús L. Romalde
Keyword(s):  
Causative Agent ◽  
Culture Media ◽  
Brown Ring Disease

Phagocytic and chemotactic responses of manila and carpet shell clam haemocytes against Vibrio tapetis, the causative agent of brown ring disease

1999 ◽  
Vol 9 (7) ◽  
pp. 543-555 ◽  
Author(s):  
Lourdes Lopez-Cortes ◽  
Dolores Castro ◽  
Jose I Navas ◽  
Juan J Borrego
Keyword(s):  
Causative Agent ◽  
Brown Ring Disease

scholarly journals Root rot of anthracnose mother plants of garden strawberry: morphological and cultural characteristics of the pathogen and the search for effective fungicides

2020 ◽  
Vol 25 ◽  
pp. 06003
Author(s):  
Julia Kashchits ◽  
Galina Yakuba
Keyword(s):  
Causative Agent ◽  
Root Rot ◽  
Culture Media ◽  
Fungal Spores ◽  
Cultural Characteristics ◽  
Mother Plants ◽  
Pure Cultures ◽  
Krasnodar Region ◽  
High Diversity

On the territory of the Krasnodar region, anthracnose root rot, the causative agent Colletotrichum аcutatum Simmonds, is one of the most harmful diseases in the mother plants of garden strawberry. The death of affected plants ranges from 33 to 100 %. Purpose of the research was to study the morphological and cultural characteristics of the causative agent of anthracnose root rot in the mother plant of garden strawberries in the region and to assess the effectiveness of fungicides in controlling the pathogen. The studies were carried out at FGBNU SKFNTSSVV in 2018-2020 using generally accepted techniques. The object of the research is pure cultures of C. acutatum. The morphological and cultural characteristics of C. acutatum were studied on three culture media. A high diversity of the identified morphotypes was shown in terms of the size of conidia and conidiophores, the shape and color of apressoria, and the method of formation of acervules. The influence of seven fungicides of various chemical classes on the development of C. аcutatum spores has been assessed. Under in vitro conditions at the indicated concentrations, the preparations Luna Tranquility, SC (0,15 %), Sercadis Plus, SC (0,1 and 0,15 %), Strobi, WG (0,05 %), Horus, WG (0,04 %) and Skor, EC (0,04 and 0,05 %) completely prevented the formation of fungal spores.

Virulence factors ofVibrioP1, the causative agent of brown ring disease in the Manila clam,Ruditapes philippinarum

1996 ◽  
Vol 9 (2) ◽  
pp. 125-136 ◽  
Author(s):  
Juan J. Borrego ◽  
Antonio Luque ◽  
Dolores Castro ◽  
Jesús A. Santamaría ◽  
Eduardo Martínez-Manzanares
Keyword(s):  
Virulence Factors ◽  
Causative Agent ◽  
Ruditapes Philippinarum ◽  
Manila Clam ◽  
Brown Ring Disease

scholarly journals Lyme disease: a search for a causative agent in ticks in south–eastern Australia

1994 ◽  
Vol 112 (2) ◽  
pp. 375-384 ◽  
Author(s):  
R. C. Russell ◽  
S. L. Doggett ◽  
R. Munro ◽  
J. Ellis ◽  
D. Avery ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Causative Agent ◽  
Culture Media ◽  
Gut Contents ◽  
Causative Organism ◽  
Definitive Evidence ◽  
South Wales ◽  
Eastern Australia ◽  
Tick Vectors ◽  
Human Infections

SUMMARYAttempts were made to identify the causative organism of Lyme disease in Australia from possible tick vectors.Ticks were collected in coastal areas of New South Wales, Australia, from localities associated with putative human infections. The ticks were dissected; a portion of the gut contents was examined for spirochaetes by microscopy, the remaining portion inoculated into culture media. The detection of spirochaetes in culture was performed using microscopy, and immunochemical and molecular (PCR) techniques. Additionally, whole ticks were tested with PCR for spirochaetes.From 1990 to 1992, approximately 12000 ticks were processed for spirochaetes. No evidence ofBorrelia burgdorferior any other spirochaete was recovered from or detected in likely tick vectors. Some spirochaete–like objects detected in the cultures were shown to be artifacts, probably aggregates of bacterial flagellae.There is no definitive evidence for the existence in Australia ofB. burgdorferithe causative agent of true Lyme disease, or for any other tick–borne spirochaete that may be responsible for a local syndrome being reported as Lyme disease.

scholarly journals THE PURE CULTIVATION OF SPIROCHÆTA ICTEROHÆMORRHAGIÆ (INADA)

1916 ◽  
Vol 23 (4) ◽  
pp. 557-562 ◽  
Author(s):  
Tetsuta Ito ◽  
Haruichiro Matsuzaki
Keyword(s):  
Causative Agent ◽  
Guinea Pigs ◽  
Culture Media ◽  
Biological Properties ◽  
Facultative Anaerobe ◽  
Fluid Medium ◽  
Weil’S Disease ◽  
Pure Cultures ◽  
Weil's Disease ◽  
Different Strains

Pure cultures of the spirochætal causative agent of the disease known as Weil's disease, or febrile icterus, in Japan, have been obtained by us in a solid, a semisolid, and a fluid medium. The spirochæta thus isolated remains pathogenic for guinea pigs for many generations. Up to the present time we have succeeded through the courtesy of Professor Nagayo, Dr. Konuma, and Dr. Ishihara, in cultivating three different strains. The spirochæta is a facultative anaerobe. The solid and semisolid culture media possess one disadvantage, in that they are opaque on account of the addition of red blood corpuscles; but it is hoped that this drawback may soon be overcome by further studies. We shall report later the results of investigations regarding various questions in immunity as well as further details regarding the biological properties of the spirochæta.

scholarly journals Vibrio tapetis sp. nov., the Causative Agent of the Brown Ring Disease Affecting Cultured Clams

1996 ◽  
Vol 46 (2) ◽  
pp. 480-484 ◽  
Author(s):  
J. J. BORREGO ◽  
D. CASTRO ◽  
A. LUQUE ◽  
C. PAILLARD ◽  
P. MAES ◽  
...  
Keyword(s):  
Causative Agent ◽  
Brown Ring Disease

Development of immunological techniques for the detection of the potential causative agent of the brown ring disease

Aquaculture ◽  
1995 ◽  
Vol 132 (1-2) ◽  
pp. 97-104 ◽  
Author(s):  
D. Castro ◽  
A. Luque ◽  
J.A. Santamaría ◽  
P. Maes ◽  
E. Martínez-Manzanares ◽  
...  
Keyword(s):  
Causative Agent ◽  
Brown Ring Disease
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