scholarly journals Real-time tissue offset correction system for intravital multiphoton microscopy

2016 ◽  
Vol 438 ◽  
pp. 35-41 ◽  
Author(s):  
Mykhailo Vladymyrov ◽  
Jun Abe ◽  
Federica Moalli ◽  
Jens V. Stein ◽  
Akitaka Ariga
Keyword(s):  
Real Time ◽  
Multiphoton Microscopy ◽  
Offset Correction ◽  
Correction System

scholarly journals Real Time Trajectory Correction System Of Optical Head In Laser Welding

2015 ◽  
Vol 9 (4) ◽  
pp. 265-269 ◽  
Author(s):  
Wojciech Cieszyński ◽  
Michał Zięba ◽  
Jacek Reiner
Keyword(s):  
Laser Beam ◽  
Laser Welding ◽  
Real Time ◽  
Laser Processing ◽  
High Accuracy ◽  
Precise Positioning ◽  
Optical Head ◽  
Welding Technology ◽  
Trajectory Correction ◽  
Correction System

Abstract Application of laser welding technology requires that the laser beam is guided through the whole length of the joint with sufficiently high accuracy. This paper describes result of research on development of optomechatronic system that allows for the precise positioning of the laser head’s TCP point on the edge of welded elements during laser processing. The developed system allows for compensation of workpiece’s fixture inaccuracies, precast distortions and workpiece deformations occurring during the process.

scholarly journals Compact portable multiphoton microscopy reveals histopathological hallmarks of unprocessed lung tumor tissue in real time

2020 ◽  
Vol 2 (4) ◽  
Author(s):  
Laura M. G. Huizen ◽  
Teodora Radonic ◽  
Frank Mourik ◽  
Danielle Seinstra ◽  
Chris Dickhoff ◽  
...  
Keyword(s):  
Real Time ◽  
Tumor Tissue ◽  
Lung Tumor ◽  
Multiphoton Microscopy

ANATOMIC FOUNDATIONS FOR NERVE SPARING ROBOTIC PROSTATECTOMY: CORRELATIONS BETWEEN ANATOMIC, SURGICAL AND “REAL TIME TISSUE RECOGNITION” WITH MULTIPHOTON MICROSCOPY

2008 ◽  
Vol 179 (4S) ◽  
pp. 496-497 ◽  
Author(s):  
Ashutosh K Tewari ◽  
Rajiv Yadav ◽  
Atsushi Takenaka ◽  
Georg Bartsch ◽  
Jay K Jhaveri ◽  
...  
Keyword(s):  
Real Time ◽  
Multiphoton Microscopy ◽  
Robotic Prostatectomy ◽  
Nerve Sparing

2004 MULTIPHOTON MICROSCOPY OF RAT TESTIS FOR REAL-TIME SPERMATOGENESIS IDENTIFICATION

2011 ◽  
Vol 185 (4S) ◽  
Author(s):  
Ranjith Ramasamy ◽  
Erik Fisher ◽  
Joshua Sterling ◽  
Sushmita Mukherjee ◽  
David Huland ◽  
...  
Keyword(s):  
Real Time ◽  
Multiphoton Microscopy ◽  
Rat Testis

scholarly journals Real time closed orbit correction system

2003 ◽  
Author(s):  
L.H. Yu ◽  
R. Biscardi ◽  
J. Bittner ◽  
E. Bozoki ◽  
J. Galayda ◽  
...  
Keyword(s):  
Real Time ◽  
Closed Orbit ◽  
Orbit Correction ◽  
Correction System

scholarly journals Imaging IGF-I uptake in growth plate cartilage using in vivo multiphoton microscopy

2017 ◽  
Vol 123 (5) ◽  
pp. 1101-1109 ◽  
Author(s):  
Maria A. Serrat ◽  
Gabriela Ion
Keyword(s):  
Real Time ◽  
Growth Plate ◽  
Multiphoton Microscopy ◽  
Biologically Active ◽  
Growth Plate Chondrocytes ◽  
Growth Plates ◽  
Metatarsal Bones ◽  
Wide Range ◽  
Igf I

Bones elongate through endochondral ossification in cartilaginous growth plates located at ends of primary long bones. Linear growth ensues from a cascade of biochemical signals initiated by actions of systemic and local regulators on growth plate chondrocytes. Although cellular processes are well defined, there is a fundamental gap in understanding how growth regulators are physically transported from surrounding blood vessels into and through dense, avascular cartilage matrix. Intravital imaging using in vivo multiphoton microscopy is one promising strategy to overcome this barrier by quantitatively tracking molecular delivery to cartilage from the vasculature in real time. We previously used in vivo multiphoton imaging to show that hindlimb heating increases vascular access of large molecules to growth plates using 10-, 40-, and 70-kDa dextran tracers. To comparatively evaluate transport of similarly sized physiological regulators, we developed and validated methods for measuring uptake of biologically active IGF-I into proximal tibial growth plates of live 5-wk-old mice. We demonstrate that fluorescently labeled IGF-I (8.2 kDa) is readily taken up in the growth plate and localizes to chondrocytes. Bioactivity tests performed on cultured metatarsal bones confirmed that the labeled protein is functional, assessed by phosphorylation of its signaling kinase, Akt. This methodology, which can be broadly applied to many different proteins and tissues, is relevant for understanding factors that affect delivery of biologically relevant molecules to the skeleton in real time. Results may lead to the development of drug-targeting strategies to treat a wide range of bone and cartilage pathologies. NEW & NOTEWORTHY This paper describes and validates a novel method for imaging transport of biologically active, fluorescently labeled IGF-I into skeletal growth plates of live mice using multiphoton microscopy. Cellular patterns of fluorescence in the growth plate were completely distinct from our prior publications using biologically inert probes, demonstrating for the first time in vivo localization of IGF-I in chondrocytes and perichondrium. These results form important groundwork for future studies aimed at targeting therapeutics into growth plates.

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