Preparation of monoclonal antibodies against poor immunogenic avian influenza virus proteins

2013 ◽  
Vol 387 (1-2) ◽  
pp. 43-50 ◽  
Author(s):  
Jie-Long He ◽  
Ming-Shou Hsieh ◽  
Yi-Chung Chiu ◽  
Rong-Huay Juang ◽  
Ching-Ho Wang
Keyword(s):  
Influenza Virus ◽  
Monoclonal Antibodies ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Virus Proteins

Production and Characterization of Monoclonal Antibodies Against Nucleoprotein of Avian Influenza Virus

2013 ◽  
Vol 32 (6) ◽  
pp. 413-418 ◽  
Author(s):  
Sushant Bhat ◽  
Sandeep Bhatia ◽  
Richa Sood ◽  
Himanshu Bhatnagar ◽  
Atul Pateriya ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Monoclonal Antibodies ◽  
Avian Influenza ◽  
Avian Influenza Virus

scholarly journals Antigen-capture ELISA and immunochromatographic test strip to detect the H9N2 subtype avian influenza virus rapidly based on monoclonal antibodies

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Yixin Xiao ◽  
Fan Yang ◽  
Fumin Liu ◽  
Hangping Yao ◽  
Nanping Wu ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Monoclonal Antibodies ◽  
Detection Limit ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Rapid Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Test ◽  
H9n2 Subtype ◽  
Antigen Capture

Abstract Background The H9N2 subtype of avian influenza virus (AIV) has become the most widespread subtype of AIV among birds in Asia, which threatens the poultry industry and human health. Therefore, it is important to establish methods for the rapid diagnosis and continuous surveillance of H9N2 subtype AIV. Methods In this study, an antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) and a colloidal gold immunochromatographic test (ICT) strip using monoclonal antibodies (MAbs) 3G4 and 2G7 were established to detect H9N2 subtype AIV. Results The AC-ELISA method and ICT strip can detect H9N2 subtype AIV quickly, and do not cross-react with other subtype AIVs or other viruses. The detection limit of AC-ELISA was a hemagglutinin (HA) titer of 4 for H9N2 subtype AIV per 100 μl sample, and the limit of detection of the HA protein of AIV H9N2 was 31.5 ng/ml. The ICT strip detection limit was an HA titer of 4 for H9N2 subtype AIV per 100 μl sample. Moreover, both detection methods exhibited good reproducibility and repeatability, with coefficients of variation < 5%. For detection in 200 actual poultry samples, the sensitivities and specificities of AC-ELISA were determined as 93.2% and 98.1%, respectively. The sensitivities and specificities of the ICT strips were determined as 90.9% and 97.4%, respectively. Conclusions The developed AC-ELISA and ICT strips displayed high specificity, sensitivity, and stability, making them suitable for rapid diagnosis and field investigation of H9N2 subtype AIV.

Immunological Detection of Avian Influenza Virus in Infected Ducks by Monoclonal Antibodies Against AIV-H5N1

2007 ◽  
Vol 1 (3) ◽  
pp. 119-124
Author(s):  
NYOMAN MANTIK ASTAWA ◽  
IDA BAGUS OKA WINAYA ◽  
LUH PUTU AGUSTINI ◽  
NINING HARTANINGSIH
Keyword(s):  
Influenza Virus ◽  
Monoclonal Antibodies ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Immunological Detection

Development and application of monoclonal antibodies against avian influenza virus nucleoprotein

2008 ◽  
Vol 147 (2) ◽  
pp. 265-274 ◽  
Author(s):  
Ming Yang ◽  
Yohannes Berhane ◽  
Tim Salo ◽  
Mingyi Li ◽  
Kate Hole ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Monoclonal Antibodies ◽  
Avian Influenza ◽  
Avian Influenza Virus
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