Antibody immobilization using pneumatic spray: Comparison with the avidin–biotin bridge immobilization method

2012 ◽  
Vol 386 (1-2) ◽  
pp. 1-9 ◽  
Author(s):  
Jhon Figueroa ◽  
Sonia Magaña ◽  
Daniel V. Lim ◽  
Rudy Schlaf
Keyword(s):  
Antibody Immobilization ◽  
Immobilization Method

New antibody immobilization method via functional liposome layer for specific protein assays

2005 ◽  
Vol 21 (5) ◽  
pp. 833-838 ◽  
Author(s):  
H.Y. Lee ◽  
H.S. Jung ◽  
K. Fujikawa ◽  
J.W. Park ◽  
J.M. Kim ◽  
...  
Keyword(s):  
Specific Protein ◽  
Antibody Immobilization ◽  
Protein Assays ◽  
Immobilization Method

scholarly journals Immunomodulating nano-adaptors potentiate antibody-based cancer immunotherapy

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Cheng-Tao Jiang ◽  
Kai-Ge Chen ◽  
An Liu ◽  
Hua Huang ◽  
Ya-Nan Fan ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Immune Responses ◽  
Cancer Immunotherapy ◽  
Tumor Cells ◽  
Noncovalent Interactions ◽  
Killer Cell ◽  
Antibody Immobilization ◽  
Effector Cells ◽  
Nanoparticle Surface

AbstractModulating effector immune cells via monoclonal antibodies (mAbs) and facilitating the co-engagement of T cells and tumor cells via chimeric antigen receptor- T cells or bispecific T cell-engaging antibodies are two typical cancer immunotherapy approaches. We speculated that immobilizing two types of mAbs against effector cells and tumor cells on a single nanoparticle could integrate the functions of these two approaches, as the engineered formulation (immunomodulating nano-adaptor, imNA) could potentially associate with both cells and bridge them together like an ‘adaptor’ while maintaining the immunomodulatory properties of the parental mAbs. However, existing mAbs-immobilization strategies mainly rely on a chemical reaction, a process that is rough and difficult to control. Here, we build up a versatile antibody immobilization platform by conjugating anti-IgG (Fc specific) antibody (αFc) onto the nanoparticle surface (αFc-NP), and confirm that αFc-NP could conveniently and efficiently immobilize two types of mAbs through Fc-specific noncovalent interactions to form imNAs. Finally, we validate the superiority of imNAs over the mixture of parental mAbs in T cell-, natural killer cell- and macrophage-mediated antitumor immune responses in multiple murine tumor models.

scholarly journals Simultaneous Bacillus anthracis Spores Detection via Aminated-Poly(vinyl chloride) Coated Piezoelectric Crystal Immunosensor

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Ali Oztuna ◽  
Hasan Nazir ◽  
Mehmet Baysallar
Keyword(s):  
Bacillus Anthracis ◽  
Vinyl Chloride ◽  
Quartz Crystal ◽  
Adhesive Layer ◽  
Antibody Immobilization ◽  
Potential Threat ◽  
Amine Functionalized ◽  
Poly Vinyl Chloride ◽  
Bacillus Anthracis Spores ◽  
Flow Through

Bacillus anthracis spores are a potential threat to countries in the context of biodefense. We have already seen the destructiveness of the anthrax attacks in the recent past. This study presents an aminated-poly(vinyl chloride) (PVC-NH2) coated quartz crystal microbalance (QCM) immunosensor for simultaneous rapid detection of B. anthracis spores. PVC-NH2, synthesized in the laboratory, was used as an adhesive layer for monoclonal antibody immobilization on gold quartz crystal. The prepared QCM sensor was tested using a pathogen field strain of B. anthracis (GenBank number: GQ375871.1) under static addition and flow through procedures with different spore concentrations. Fourier transform infrared spectroscopy (FTIR-ATR) and scanning electron microscopy (SEM) were performed to characterize the surface of the sensor during the modification. Furthermore, a series of SEM micrographs were taken in order to investigate surface morphology and show the presence of the B. anthracis spores on the surface. It is concluded that B. anthracis spores can be accomplished by using amine functionalized polymer coated QCM sensors without requiring complicated immobilization procedures or expensive preliminary preparations.

scholarly journals Modification of silicon nitride surfaces with GOPES and APTES for antibody immobilization: computational and experimental studies

2015 ◽  
Vol 6 (4) ◽  
pp. 045006 ◽  
Author(s):  
Thien Dien To ◽  
Anh Tuan Nguyen ◽  
Khoa Nhat Thanh Phan ◽  
An Thu Thi Truong ◽  
Tin Chanh Duc Doan ◽  
...  
Keyword(s):  
Silicon Nitride ◽  
Experimental Studies ◽  
Antibody Immobilization

Plasma functionalization procedure for antibody immobilization for SU-8 based sensor

2016 ◽  
Vol 86 ◽  
pp. 827-833 ◽  
Author(s):  
Immacolata Angelica Grimaldi ◽  
Genni Testa ◽  
Gianluca Persichetti ◽  
Fausta Loffredo ◽  
Fulvia Villani ◽  
...  
Keyword(s):  
Antibody Immobilization ◽  
Plasma Functionalization

Improvement of antibody immobilization using hyperbranched polymer and protein A

2011 ◽  
Vol 409 (1) ◽  
pp. 22-27 ◽  
Author(s):  
Guangyu Shen ◽  
Chenbo Cai ◽  
Kun Wang ◽  
Jilin Lu
Keyword(s):  
Hyperbranched Polymer ◽  
Protein A ◽  
Antibody Immobilization

Development of peptide immobilization method using PS-tagged cushion protein

2009 ◽  
Vol 108 ◽  
pp. S161
Author(s):  
Hiroyuki Imanaka ◽  
Daisuke Yamazumi ◽  
Toshinobu Kunikata ◽  
Koreyoshi Imamura ◽  
Kazuhiro Nakanishi
Keyword(s):  
Immobilization Method

scholarly journals Tunable control of antibody immobilization using electric field

2015 ◽  
Vol 112 (7) ◽  
pp. 1995-1999 ◽  
Author(s):  
Sam Emaminejad ◽  
Mehdi Javanmard ◽  
Chaitanya Gupta ◽  
Shuai Chang ◽  
Ronald W. Davis ◽  
...  
Keyword(s):  
Solid State ◽  
Electric Field ◽  
Signal To Noise Ratio ◽  
Physical Adsorption ◽  
Antibody Immobilization ◽  
Proof Of Concept ◽  
Force Microscopy ◽  
Atomic Force ◽  
Sensing Platforms ◽  
Glass Surfaces

The controlled immobilization of proteins on solid-state surfaces can play an important role in enhancing the sensitivity of both affinity-based biosensors and probe-free sensing platforms. Typical methods of controlling the orientation of probe proteins on a sensor surface involve surface chemistry-based techniques. Here, we present a method of tunably controlling the immobilization of proteins on a solid-state surface using electric field. We study the ability to orient molecules by immobilizing IgG molecules in microchannels while applying lateral fields. We use atomic force microscopy to both qualitatively and quantitatively study the orientation of antibodies on glass surfaces. We apply this ability for controlled orientation to enhance the performance of affinity-based assays. As a proof of concept, we use fluorescence detection to indirectly verify the modulation of the orientation of proteins bound to the surface. We studied the interaction of fluorescently tagged anti-IgG with surface immobilized IgG controlled by electric field. Our study demonstrates that the use of electric field can result in more than 100% enhancement in signal-to-noise ratio compared with normal physical adsorption.

Comparison of antibody immobilization strategies in detection ofVibrio choleraeby surface plasmon resonance

2016 ◽  
Vol 11 (4) ◽  
pp. 041006 ◽  
Author(s):  
Ramezan Ali Taheri ◽  
Ali Hossein Rezayan ◽  
Fereshteh Rahimi ◽  
Javad Mohammadnejad ◽  
Mehdi Kamali
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Antibody Immobilization

scholarly journals Advantages of the Biomimetic Nanostructured Films as an Immobilization Method vs. the Carbon Paste Classical Method

Catalysts ◽  
2012 ◽  
Vol 2 (4) ◽  
pp. 517-531 ◽  
Author(s):  
Constantin Apetrei ◽  
Jose Saja ◽  
Javier Zurro ◽  
Maria Rodríguez-Méndez
Keyword(s):  
Classical Method ◽  
Carbon Paste ◽  
Nanostructured Films ◽  
Immobilization Method
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