scholarly journals An improved cell isolation method for flow cytometric and functional analyses of cutaneous wound leukocytes

2011 ◽  
Vol 373 (1-2) ◽  
pp. 161-166 ◽  
Author(s):  
Aleah L. Brubaker ◽  
David F. Schneider ◽  
Jessica L. Palmer ◽  
Douglas E. Faunce ◽  
Elizabeth J. Kovacs
Keyword(s):  
Cell Isolation ◽  
Isolation Method ◽  
Cutaneous Wound ◽  
Flow Cytometric ◽  
Functional Analyses

Fetal Cell Isolation from Maternal Blood Cultures by Flow Cytometric Hemoglobin Profiles

2002 ◽  
Vol 17 (2) ◽  
pp. 83-89 ◽  
Author(s):  
Ralph M. Bohmer ◽  
Helene P. Stroh ◽  
Kirby L. Johnson ◽  
Erik S. LeShane ◽  
Diana W. Bianchi
Keyword(s):  
Maternal Blood ◽  
Cell Isolation ◽  
Blood Cultures ◽  
Fetal Cell ◽  
Flow Cytometric

scholarly journals A universal tumor cell isolation method enabled by fibrin-coated microchannels

The Analyst ◽  
2016 ◽  
Vol 141 (2) ◽  
pp. 563-566 ◽  
Author(s):  
Jinling Zhang ◽  
Z. Hugh Fan
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Cell Isolation ◽  
Mesenchymal Tumor ◽  
Isolation Method

We found that the fibrin-immobilized surfaces on microchannels were able to capture both epithelial and mesenchymal tumor cells.

scholarly journals Precardiac deletion of Numb and Numblike reveals renewal of cardiac progenitors

eLife ◽  
2014 ◽  
Vol 3 ◽  
Author(s):  
Lincoln T Shenje ◽  
Peter Andersen ◽  
Hideki Uosaki ◽  
Laviel Fernandez ◽  
Peter P Rainer ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Cell Fate ◽  
Cardiac Cells ◽  
Cardiac Progenitor Cells ◽  
Pharyngeal Arches ◽  
Cardiac Progenitors ◽  
Intrinsic Factors ◽  
Flow Cytometric ◽  
Functional Analyses

Cardiac progenitor cells (CPCs) must control their number and fate to sustain the rapid heart growth during development, yet the intrinsic factors and environment governing these processes remain unclear. Here, we show that deletion of the ancient cell-fate regulator Numb (Nb) and its homologue Numblike (Nbl) depletes CPCs in second pharyngeal arches (PA2s) and is associated with an atrophic heart. With histological, flow cytometric and functional analyses, we find that CPCs remain undifferentiated and expansive in the PA2, but differentiate into cardiac cells as they exit the arch. Tracing of Nb- and Nbl-deficient CPCs by lineage-specific mosaicism reveals that the CPCs normally populate in the PA2, but lose their expansion potential in the PA2. These findings demonstrate that Nb and Nbl are intrinsic factors crucial for the renewal of CPCs in the PA2 and that the PA2 serves as a microenvironment for their expansion.

scholarly journals 0363 : Development of a cell isolation method in large mammals: regional differences in calcium signaling across left atrium from sheep

2015 ◽  
Vol 7 (2) ◽  
pp. 164-165
Author(s):  
Caroline Cros ◽  
Caroline Pascarel-Auclerc ◽  
David Benoist ◽  
Olivier Bernus ◽  
Pierre Jais ◽  
...  
Keyword(s):  
Calcium Signaling ◽  
Left Atrium ◽  
Regional Differences ◽  
Cell Isolation ◽  
Large Mammals ◽  
Isolation Method ◽  
A Cell

scholarly journals Collagen type I and decorin expression in tenocytes depend on the cell isolation method

2012 ◽  
Vol 13 (1) ◽  
Author(s):  
Markus U Wagenhäuser ◽  
Matthias F Pietschmann ◽  
Birte Sievers ◽  
Denitsa Docheva ◽  
Matthias Schieker ◽  
...  
Keyword(s):  
Collagen Type ◽  
Collagen Type I ◽  
Cell Isolation ◽  
Type I ◽  
Isolation Method

scholarly journals Femtosecond laser microdissection isolating regenerating C. elegans neurons for single cell RNA sequencing

2021 ◽  
Author(s):  
Adela Ben-Yakar ◽  
Peisen Zhao ◽  
Chris Martin ◽  
Ke-Yue Ma ◽  
Ning Jiang
Keyword(s):  
Femtosecond Laser ◽  
Single Cell ◽  
Rna Sequencing ◽  
Single Neuron ◽  
Laser Microdissection ◽  
Cell Isolation ◽  
Isolation Method ◽  
C Elegans ◽  
Single Cell Rna Sequencing ◽  
Single Cell Isolation

Abstract Our understanding of nerve regeneration can be enhanced by delineating its underlying molecular activities at single neuron resolution in small model organisms such as Caenorhabditis elegans. Existing cell isolation techniques cannot isolate regenerating neurons from the nematode. We present femtosecond laser microdissection (fs-LM), a new single cell isolation method that dissects intact cells directly from living tissue by leveraging the micron-scale precision of fs-laser ablation. We show that fs-LM facilitated sensitive and specific gene expression profiling by single cell RNA-sequencing, while mitigating the stress related transcriptional artifacts induced by tissue dissociation. Single cell RNA-sequencing of fs-LM isolated regenerating C. elegans neurons revealed transcriptional program leading to successful regeneration in wild-type animals or regeneration failure in animals lacking DLK-1/p38 kinase. The ability of fs-LM to isolate specific neurons based on phenotype of interest allowed us to study the molecular basis of regeneration heterogeneity displayed by neurons of the same type. We identified gene modules whose expression patterns were correlated with axon regrowth rate at a single neuron level. Our results establish fs-LM as a highly specific single cell isolation method ideal for precision and phenotype-driven studies.

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