Small interfering RNA (siRNA) delivery into monocyte-derived dendritic cells by electroporation

2006 ◽  
Vol 311 (1-2) ◽  
pp. 139-152 ◽  
Author(s):  
Alexander T. Prechtel ◽  
Nadine M. Turza ◽  
Alexandros A. Theodoridis ◽  
Mirko Kummer ◽  
Alexander Steinkasserer
Keyword(s):  
Dendritic Cells ◽  
Small Interfering Rna ◽  
Sirna Delivery ◽  
Interfering Rna

Small interfering RNA (siRNA) delivery into murine bone marrow-derived dendritic cells by electroporation

2008 ◽  
Vol 337 (1) ◽  
pp. 71-77 ◽  
Author(s):  
Jonathan Jantsch ◽  
Nadine Turza ◽  
Melanie Volke ◽  
Kai-Uwe Eckardt ◽  
Michael Hensel ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Dendritic Cells ◽  
Small Interfering Rna ◽  
Sirna Delivery ◽  
Murine Bone Marrow ◽  
Interfering Rna

scholarly journals pH-sensitive zwitterionic polycarboxybetaine as a potential non-viral vector for small interfering RNA delivery

RSC Advances ◽  
2020 ◽  
Vol 10 (73) ◽  
pp. 45059-45066
Author(s):  
Huan Peng ◽  
Weihong Ji ◽  
Ruichen Zhao ◽  
Zhiguo Lu ◽  
Jun Yang ◽  
...  
Keyword(s):  
Small Interfering Rna ◽  
Viral Vector ◽  
Sirna Delivery ◽  
Ph Sensitive ◽  
Acidic Environment ◽  
Interfering Rna

pH-sensitive zwitterionic polycarboxybetaine could complex siRNA in an acidic environment and could be used as a non-viral vector for safe siRNA delivery.

scholarly journals Structurally modulated codelivery of siRNA and Argonaute 2 for enhanced RNA interference

2018 ◽  
Vol 115 (12) ◽  
pp. E2696-E2705 ◽  
Author(s):  
Jiahe Li ◽  
Connie Wu ◽  
Wade Wang ◽  
Yanpu He ◽  
Elad Elkayam ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Rna Interference ◽  
Small Interfering Rna ◽  
Sirna Delivery ◽  
Effector Protein ◽  
Side Group ◽  
Argonaute 2 ◽  
Fundamental Research ◽  
Delivery Vehicles ◽  
Interfering Rna

Small interfering RNA (siRNA) represents a promising class of inhibitors in both fundamental research and the clinic. Numerous delivery vehicles have been developed to facilitate siRNA delivery. Nevertheless, achieving highly potent RNA interference (RNAi) toward clinical translation requires efficient formation of RNA-induced gene-silencing complex (RISC) in the cytoplasm. Here we coencapsulate siRNA and the central RNAi effector protein Argonaute 2 (Ago2) via different delivery carriers as a platform to augment RNAi. The physical clustering between siRNA and Ago2 is found to be indispensable for enhanced RNAi. Moreover, by utilizing polyamines bearing the same backbone but distinct cationic side-group arrangements of ethylene diamine repeats as the delivery vehicles, we find that the molecular structure of these polyamines modulates the degree of siRNA/Ago2-mediated improvement of RNAi. We apply this strategy to silence the oncogene STAT3 and significantly prolong survival in mice challenged with melanoma. Our findings suggest a paradigm for RNAi via the synergistic coassembly of RNA with helper proteins.

scholarly journals Natural Polysaccharides for siRNA Delivery: Nanocarriers Based on Chitosan, Hyaluronic Acid, and Their Derivatives

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2570 ◽  
Author(s):  
Inés Serrano-Sevilla ◽  
Álvaro Artiga ◽  
Scott G. Mitchell ◽  
Laura De Matteis ◽  
Jesús M. de la Fuente
Keyword(s):  
Drug Delivery ◽  
Hyaluronic Acid ◽  
Small Interfering Rna ◽  
Drug Delivery Systems ◽  
Sirna Delivery ◽  
Delivery Systems ◽  
Low Toxicity ◽  
Interfering Rna

Natural polysaccharides are frequently used in the design of drug delivery systems due to their biocompatibility, biodegradability, and low toxicity. Moreover, they are diverse in structure, size, and charge, and their chemical functional groups can be easily modified to match the needs of the final application and mode of administration. This review focuses on polysaccharidic nanocarriers based on chitosan and hyaluronic acid for small interfering RNA (siRNA) delivery, which are highly positively and negatively charged, respectively. The key properties, strengths, and drawbacks of each polysaccharide are discussed. In addition, their use as efficient nanodelivery systems for gene silencing applications is put into context using the most recent examples from the literature. The latest advances in this field illustrate effectively how chitosan and hyaluronic acid can be modified or associated with other molecules in order to overcome their limitations to produce optimized siRNA delivery systems with promising in vitro and in vivo results.

scholarly journals Elucidating the role of surface chemistry on cationic phosphorus dendrimer–siRNA complexation

Nanoscale ◽  
2018 ◽  
Vol 10 (23) ◽  
pp. 10952-10962 ◽  
Author(s):  
Marco A. Deriu ◽  
Nicolas Tsapis ◽  
Magali Noiray ◽  
Gianvito Grasso ◽  
Nabil El Brahmi ◽  
...  
Keyword(s):  
Surface Chemistry ◽  
Structural Properties ◽  
Crucial Role ◽  
Small Interfering Rna ◽  
Sirna Delivery ◽  
Interfering Rna

In the field of dendrimers targeting small interfering RNA (siRNA) delivery, dendrimer structural properties, such as the surface chemistry, play a crucial role in the efficiency of complexation.

scholarly journals Culture medium used during small interfering RNA (siRNA) transfection determines the maturation status of dendritic cells

2020 ◽  
Vol 479 ◽  
pp. 112748
Author(s):  
Mieke F. van Essen ◽  
Nicole Schlagwein ◽  
Daniëlle J. van Gijlswijk-Janssen ◽  
Jacqueline D.H. Anholts ◽  
Michael Eikmans ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Small Interfering Rna ◽  
Culture Medium ◽  
Sirna Transfection ◽  
Interfering Rna

scholarly journals Switching the intracellular pathway and enhancing the therapeutic efficacy of small interfering RNA by auroliposome

2020 ◽  
Vol 6 (30) ◽  
pp. eaba5379 ◽  
Author(s):  
Md. Nazir Hossen ◽  
Lin Wang ◽  
Harisha R. Chinthalapally ◽  
Joe D. Robertson ◽  
Kar-Ming Fung ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Gold Nanoparticles ◽  
Gene Silencing ◽  
Small Interfering Rna ◽  
Sirna Delivery ◽  
Delivery Systems ◽  
Ovarian Cancer Cells ◽  
Interfering Rna

Gene silencing using small-interfering RNA (siRNA) is a viable therapeutic approach; however, the lack of effective delivery systems limits its clinical translation. Herein, we doped conventional siRNA-liposomal formulations with gold nanoparticles to create “auroliposomes,” which significantly enhanced gene silencing. We targeted MICU1, a novel glycolytic switch in ovarian cancer, and delivered MICU1-siRNA using three delivery systems—commercial transfection agents, conventional liposomes, and auroliposomes. Low-dose siRNA via transfection or conventional liposomes was ineffective for MICU1 silencing; however, in auroliposomes, the same dose gave >85% gene silencing. Efficacy was evident from both in vitro growth assays of ovarian cancer cells and in vivo tumor growth in human ovarian cell line—and patient-derived xenograft models. Incorporation of gold nanoparticles shifted intracellular uptake pathways such that liposomes avoided degradation within lysosomes. Auroliposomes were nontoxic to vital organs. Therefore, auroliposomes represent a novel siRNA delivery system with superior efficacy for multiple therapeutic applications.

scholarly journals CD83 Knockdown in Monocyte-Derived Dendritic Cells by Small Interfering RNA Leads to a Diminished T Cell Stimulation

2007 ◽  
Vol 178 (9) ◽  
pp. 5454-5464 ◽  
Author(s):  
Alexander T. Prechtel ◽  
Nadine M. Turza ◽  
Alexandros A. Theodoridis ◽  
Alexander Steinkasserer
Keyword(s):  
Dendritic Cells ◽  
T Cell ◽  
Small Interfering Rna ◽  
Cell Stimulation ◽  
T Cell Stimulation ◽  
Interfering Rna

A biodegradable amphiphilic poly(aminoester) dendrimer for safe and effective siRNA delivery

2022 ◽  
Author(s):  
Chi Ma ◽  
Dandan Zhu ◽  
Wenyi Lin ◽  
Ying Li ◽  
Yuanzheng Huang ◽  
...  
Keyword(s):  
Small Interfering Rna ◽  
Sirna Delivery ◽  
Interfering Rna

Small interfering RNA (siRNA)-based therapeutics represent a novel and compelling drug modality, provided that safe and competent vectors are available for their delivery. Here, we report a biodegradable amphiphilic poly(aminoester)...

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