scholarly journals 603 Handheld dual-modality ultrasound and photoacoustic imaging for in vivo determination of melanoma depth

2016 ◽  
Vol 136 (5) ◽  
pp. S107
Author(s):  
S.V. Tripathi ◽  
Y. Zhou ◽  
G.P. Linette ◽  
I.S. Rosman ◽  
M. Council ◽  
...  
Keyword(s):  
Photoacoustic Imaging ◽  
Dual Modality

scholarly journals Organic nanoparticle-doped microdroplets as dual-modality contrast agents for ultrasound microvascular flow and photoacoustic imaging

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yu Xu ◽  
Guoyun Sun ◽  
Eshu Middha ◽  
Yu-Hang Liu ◽  
Kim Chuan Chan ◽  
...  
Keyword(s):  
Blood Flow ◽  
Nude Mice ◽  
Photoacoustic Imaging ◽  
Imaging Techniques ◽  
Contrast Effects ◽  
In Vivo Evaluation ◽  
Dual Modality ◽  
In Vivo Experiments

Abstract Tumor blood vessels are chaotic and abundantly distributed, owing to their heterogeneity. Therefore, imaging techniques which reveal abnormalities of tumor vasculature play significant roles in both mechanistic and clinical diagnostic tumor studies. Photoacoustic (PA) imaging uses the intrinsic characteristics of hemoglobin, to acquire tumor hemodynamic information, while ultrasound (US) imaging provides information about tumoral vessel structures and blood flow. To improve the imaging contrast performance, hydrogel-based microdroplets were designed for both US blood flow and PA imaging in this study. The microdroplets served as carriers for PA contrast agent solution in the innermost part while oil and hydrogel formed the inner and outer layers of the droplets. In vitro experiments firstly demonstrated the dual modality contrast effects of the microdroplets on US flow determination and PA imaging. In vivo experiments were then carried out in both healthy nude mice and nude mice with subcutaneous tumor to validate the contrast effects and to monitor the duration of contrast effects in animals. Using the dual-modality microdroplets, we were able to obtain distinct edges of tumor and blood flow mapping of the tumor microvascular with improved sensitivity up to 11.09 dB for PA and 6.69 dB for US flow. Besides, the in vivo evaluation with microdroplets showed US flow enhancement for more than 60 min. Therefore, the microdroplets are able to provide the contrast effects for both US flow and PA in a relative long duration and have potential to be applied in the tumor related diagnoses and studies.

A ratiometric photoacoustic imaging approach for semi-quantitative determination of aggregation efficiency in vivo

Nanoscale ◽  
2020 ◽  
Vol 12 (36) ◽  
pp. 18654-18662
Author(s):  
Bo Peng ◽  
Xiu-Mei Liu ◽  
Hsian-Rong Tseng ◽  
Li-Li Li ◽  
Hao Wang
Keyword(s):  
Quantitative Determination ◽  
Real Time ◽  
Pancreatic Tumor ◽  
Photoacoustic Imaging ◽  
High Contrast ◽  
Imaging Approach

We first proposed a ratiometric photoacoustic (PA) imaging approach for real-time semi-quantitative calculated aggregation efficiency in vivo. The designed probe with high contrast will used for pancreatic tumor bioimaging or operation navigation.

782 Determination of Differential Tumour Vascular Pathophysiology in Vivo by Photoacoustic Imaging

2012 ◽  
Vol 48 ◽  
pp. S186-S187 ◽  
Author(s):  
S.P. Johnson ◽  
J.G. Laufer ◽  
E.Z. Zhang ◽  
P.C. Beard ◽  
R.B. Pedleys
Keyword(s):  
Photoacoustic Imaging

scholarly journals Carbon-Coated Magnetic Nanoparticle Dedicated to MRI/Photoacoustic Imaging of Tumor in Living Mice

2021 ◽  
Vol 9 ◽  
Author(s):  
Yujing Li ◽  
Fei Ye ◽  
Shanxiang Zhang ◽  
Wenjun Ni ◽  
Liewei Wen ◽  
...  
Keyword(s):  
Near Infrared ◽  
Photoacoustic Imaging ◽  
Multimodality Imaging ◽  
Morphological Structure ◽  
Quantitative Information ◽  
Dual Modality ◽  
Carbon Coated ◽  
Ferromagnetic Cobalt

Multimodality imaging can reveal complementary anatomic and functional information as they exploit different contrast mechanisms, which has broad clinical applications and promises to improve the accuracy of tumor diagnosis. Accordingly, to attain the particular goal, it is critical to exploit multimodal contrast agents. In the present work, we develop novel cobalt core/carbon shell–based nanoparticles (Cobalt at carbon NPs) with both magnetization and light absorption properties for dual-modality magnetic resonance imaging (MRI) and photoacoustic imaging (PAI). The nanoparticle consists of ferromagnetic cobalt particles coated with carbon for biocompatibility and optical absorption. In addition, the prepared Cobalt at carbon NPs are characterized by transmission electron microscope (TEM), visible–near-infrared spectra, Raman spectrum, and X-ray powder diffraction for structural analysis. Experiments verify that Cobalt at carbon NPs have been successfully constructed and the designed Cobalt at carbon NPs can be detected by both MRI and PAI in vitro and in vivo. Importantly, intravenous injection of Cobalt at carbon NPs into glioblastoma-bearing mice led to accumulation and retention of Cobalt at carbon NPs in the tumors. Using such a multifunctional probe, MRI can screen rapidly to identify potential lesion locations, whereas PAI can provide high-resolution morphological structure and quantitative information of the tumor. The Cobalt at carbon NPs are likely to become a promising candidate for dual-modality MRI/PAI of the tumor.

A Molecular Approach to Immunoscintigraphy: A Study of the T-Antigen Conformation on the Surface of Tumors

1987 ◽  
Vol 26 (01) ◽  
pp. 1-6 ◽  
Author(s):  
S. Selvaraj ◽  
M. R. Suresh ◽  
G. McLean ◽  
D. Willans ◽  
C. Turner ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Tumor Cell ◽  
T Antigen ◽  
Human Carcinomas ◽  
Animal Tumor ◽  
Synthetic Antigens

The role of glycoconjugates in tumor cell differentiation has been well documented. We have examined the expression of the two anomers of the Thomsen-Friedenreich antigen on the surface of human, canine and murine tumor cell membranes both in vitro and in vivo. This has been accomplished through the synthesis of the disaccharide terminal residues in both a and ß configuration. Both entities were used to generate murine monoclonal antibodies which recognized the carbohydrate determinants. The determination of fine specificities of these antibodies was effected by means of cellular uptake, immunohistopathology and immunoscintigraphy. Examination of pathological specimens of human and canine tumor tissue indicated that the expressed antigen was in the β configuration. More than 89% of all human carcinomas tested expressed the antigen in the above anomeric form. The combination of synthetic antigens and monoclonal antibodies raised specifically against them provide us with invaluable tools for the study of tumor marker expression in humans and their respective animal tumor models.

Storage of Human Blood Platelets

1974 ◽  
Vol 32 (02/03) ◽  
pp. 405-416 ◽  
Author(s):  
M. R Hardeman ◽  
Carina J L. Heynens
Keyword(s):  
Storage Temperature ◽  
Platelet Rich Plasma ◽  
Blood Platelets ◽  
Storage Period ◽  
Serotonin Uptake ◽  
Hypotonic Shock ◽  
Glycolytic Intermediates

SummaryStorage experiments were performed at 4°, 25° and 37° C with platelet-rich plasma under sterile conditions. In some experiments also the effect of storing platelets at 4° C in whole blood was investigated.Before, during and after three days of storage, the platelets were tested at 37° C for their serotonin uptake and response to hypotonic shock. In addition some glycolytic intermediates were determined.A fair correlation was noticed between the serotonin uptake and hypotonic shock experiments. Both parameters were best maintained at 25° C. Also platelet counting, performed after the storage period, indicated 25° C as the best storage temperature. Determination of glycolytic intermediates did not justify any conclusion regarding the optimal storage temperature. Of the various anticoagulants studied, ACD and heparin gave the best results as to the serotonin uptake and hypotonic shock response, either with fresh or stored platelets. The use of EDTA resulted in the lowest activity, especially after storage.The results of these storage experiments in vitro, correspond well with those in vivo reported in the literature.

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