scholarly journals The beneficial effect of ginsenosides extracted by pulsed electric field against hydrogen peroxide-induced oxidative stress in HEK-293 cells

2017 ◽  
Vol 41 (2) ◽  
pp. 169-179 ◽  
Author(s):  
Di Liu ◽  
Ting Zhang ◽  
Zhifei Chen ◽  
Ying Wang ◽  
Shuang Ma ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Electric Field ◽  
Beneficial Effect ◽  
Pulsed Electric Field ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells

Phytochemical profiling and screening of protective effects of Artabotrys odoratissimus on H2O2 induced oxidative stress in HEK-293 cells and erythrocytes

2020 ◽  
Vol 167 (4) ◽  
pp. 471-484
Author(s):  
Meghana Pargi ◽  
Sandeep Kumar Jain Raviraj ◽  
Prashanth Narayanappa ◽  
Kumaraswamy Malleshappa Honnenahally
Keyword(s):  
Oxidative Stress ◽  
Protective Effects ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells

Effect of Curcumin on Cisplatin- and Oxaliplatin-Induced Oxidative Stress in Human Embryonic Kidney (HEK) 293 Cells

Renal Failure ◽  
2011 ◽  
Vol 33 (5) ◽  
pp. 518-523 ◽  
Author(s):  
Mostafa I. Waly ◽  
Mansour S. Al Moundhri ◽  
Badreldin H. Ali
Keyword(s):  
Oxidative Stress ◽  
Human Embryonic Kidney ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells

scholarly journals Characterization of the reversible phosphorylation and activation of ERK8

2006 ◽  
Vol 394 (1) ◽  
pp. 365-373 ◽  
Author(s):  
Iva V. Klevernic ◽  
Margaret J. Stafford ◽  
Nicholas Morrice ◽  
Mark Peggie ◽  
Simon Morton ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Growth Factor ◽  
Protein Kinase ◽  
Insect Cells ◽  
Osmotic Shock ◽  
Tyrosine Phosphatase ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells

ERK8 (extracellular-signal-regulated protein kinase 8) expressed in Escherichia coli or insect cells was catalytically active and phosphorylated at both residues of the Thr-Glu-Tyr motif. Dephosphorylation of the threonine residue by PP2A (protein serine/threonine phosphatase 2A) decreased ERK8 activity by over 95% in vitro, whereas complete dephosphorylation of the tyrosine residue by PTP1B (protein tyrosine phosphatase 1B) decreased activity by only 15–20%. Wild-type ERK8 expressed in HEK-293 cells was over 100-fold less active than the enzyme expressed in bacteria or insect cells, but activity could be increased by exposure to hydrogen peroxide, by incubation with the protein serine/threonine phosphatase inhibitor okadaic acid, or more weakly by osmotic shock. In unstimulated cells, ERK8 was monophosphorylated at Tyr-177, and exposure to hydrogen peroxide induced the appearance of ERK8 that was dually phosphorylated at both Thr-175 and Tyr-177. IGF-1 (insulin-like growth factor 1), EGF (epidermal growth factor), PMA or anisomycin had little effect on activity. In HEK-293 cells, phosphorylation of the Thr-Glu-Tyr motif of ERK8 was prevented by Ro 318220, a potent inhibitor of ERK8 in vitro. The catalytically inactive mutants ERK8[D154A] and ERK8[K42A] were not phosphorylated in HEK-293 cells or E. coli, whether or not the cells had been incubated with protein phosphatase inhibitors or exposed to hydrogen peroxide. Our results suggest that the activity of ERK8 in transfected HEK-293 cells depends on the relative rates of ERK8 autophosphorylation and dephosphorylation by one or more members of the PPP family of protein serine/threonine phosphatases. The major residue in myelin basic protein phosphorylated by ERK8 (Ser-126) was distinct from that phosphorylated by ERK2 (Thr-97), demonstrating that, although ERK8 is a proline-directed protein kinase, its specificity is distinct from ERK1/ERK2.

Sarco(endo)plasmic reticulum Ca2+ pump isoform SERCA3 is more resistant than SERCA2b to peroxide

1997 ◽  
Vol 273 (2) ◽  
pp. C420-C425 ◽  
Author(s):  
A. K. Grover ◽  
S. E. Samson ◽  
C. M. Misquitta
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Mast Cells ◽  
Inhibitory Concentration ◽  
Lymphoid Cells ◽  
Hek 293 ◽  
Aortic Endothelium ◽  
293 Cells ◽  
Plasmic Reticulum ◽  
Ic50 Values

Sarco(endo)plasmic reticulum Ca2+ pumps are encoded by genes SERCA1, SERCA2, and SERCA3. Most tissues express SERCA2 Ca2+ pumps (splice SERCA2b) which are inactivated by reactive oxygen. In contrast, SERCA3 is expressed in tissues such as tracheal epithelium, mast cells, lymphoid cells, and aortic endothelium, which are frequently exposed to oxidative stress. Therefore, we compared SERCA3 and SERCA2b proteins for their sensitivity to oxidation. We isolated microsomes from HEK-293 cells overexpressing SERCA3 or SERCA2b. We incubated the microsomes with different concentrations of hydrogen peroxide and then determined Ca2+ pump activities in them in the following three assay systems: ATP-dependent oxalate-stimulated azide-insensitive 45Ca2+ uptake by the microsomal vesicles, Ca(2+)-Mg(2+)-ATPase, and Ca(2+)-dependent acylphosphate formation. Peroxide inhibited the pump activities in microsomes with half-maximal inhibitory concentration (IC50) values of 69 +/- 14, 66 +/- 13, and 84 +/- 15 microM for the 45Ca2+ uptake, Ca(2+)-Mg(2+)-ATPase, and the acylphosphate formation reactions, respectively. However, for microsomes from SERCA3-expressing cells, the corresponding values of IC50 for peroxide were 274 +/- 47, 857 +/- 110, and 746 +/- 40 microM. Thus, in each assay system, the resistance to inactivation by peroxide was significantly (P < 0.05) higher for the SERCA3 protein than for SERCA2b. The SERCA3 resistance to oxidants may aid the cells expressing it to function during exposure to oxidative stress.

Adapted response of the antioxidant defense system to oxidative stress induced by deoxynivalenol in Hek-293 cells

Toxicon ◽  
2011 ◽  
Vol 57 (7-8) ◽  
pp. 1023-1032 ◽  
Author(s):  
Diana Dinu ◽  
Gabriela Oana Bodea ◽  
Corina Diana Ceapa ◽  
Maria Cristina Munteanu ◽  
Florentina Israel Roming ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells
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