Core–shell fluorinated methacrylate nanoparticles with Rhodamine-B for confocal microscopy and fluorescence correlation spectroscopy applications

2016 ◽  
Vol 183 ◽  
pp. 92-99 ◽  
Author(s):  
Maciej Jarzebski ◽  
Yingze Zhang ◽  
Tomasz Sliwa ◽  
Jarosław Mazuryk ◽  
Tobiasz Deptula ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Rhodamine B ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Core Shell ◽  
Fluorescence Correlation

scholarly journals Advanced processing and analysis of conventional confocal microscopy generated scanning FCS data

2017 ◽  
Author(s):  
Dominic Waithe ◽  
Falk Schneider ◽  
Jakub Chojnacki ◽  
Mathias Clausen ◽  
Dilip Shrestha ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Spatial Heterogeneity ◽  
Fluorescence Correlation Spectroscopy ◽  
Large Scale ◽  
Molecular Diffusion ◽  
Correlation Spectroscopy ◽  
The Other ◽  
Statistical Accuracy ◽  
Fluorescence Correlation ◽  
Diffusion Dynamics

AbstractScanning Fluorescence Correlation Spectroscopy (scanning FCS) is a variant of conventional point FCS that allows molecular diffusion at multiple locations to be measured simultaneously. It enables disclosure of potential spatial heterogeneity in molecular diffusion dynamics and also the acquisition of a large amount of FCS data at the same time, providing large statistical accuracy. Here, we optimize the processing and analysis of these large-scale acquired sets of FCS data. On one hand we present FoCuS-scan, scanning FCS software that provides an end-to-end solution for processing and analysing scanning data acquired on commercial turnkey confocal systems. On the other hand, we provide a thorough characterisation of large-scale scanning FCS data over its intended time-scales and applications and propose a unique solution for the bias and variance observed when studying slowly diffusing species. Our manuscript enables researchers to straightforwardly utilise scanning FCS as a powerful technique for measuring diffusion across a broad range of physiologically relevant length scales without specialised hardware or expensive software.

Fluorescence Correlation Spectroscopy for Detection of Trace Amount of Biological Agents

2002 ◽  
Author(s):  
De-Kui Qing ◽  
M. Pinar Mengu¨c¸ ◽  
Fred A. Payne ◽  
Mary-Grace C. Danao
Keyword(s):  
Confocal Microscopy ◽  
Fluorescence Correlation Spectroscopy ◽  
Trace Amount ◽  
Flow System ◽  
Detection System ◽  
Biological Agents ◽  
Correlation Spectroscopy ◽  
Special Flow ◽  
E Coli ◽  
Fluorescence Correlation

A procedure to the trace amount of biological agents in water, based on fluorescence correlation spectroscopy and confocal microscopy, is explored. A special flow system is developed to increase the probability of detecting Escherichia coli (E. coli), and confocal microscopy is used to increase the sensitivity of the detection system. It is observed that concentrations higher than 1.5×105 E. coli per milliliter (2.5×10−16 M) are detectable with the present setup. This concentration corresponds to about 1.0 nM level of Rhodamine 6-G dyes. For detection of lower concentrations, further improvements need to be implemented to increase signal/background ratio. A detailed analysis of the optical system is presented and further improvements for the procedure are discussed.

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