Dark coffee consumption protects human blood cells from spontaneous DNA damage

2019 ◽  
Vol 55 ◽  
pp. 285-295 ◽  
Author(s):  
Gudrun Pahlke ◽  
Eva Attakpah ◽  
Georg Aichinger ◽  
Katarina Ahlberg ◽  
Christina Maria Hochkogler ◽  
...  
Keyword(s):  
Dna Damage ◽  
Human Blood ◽  
Blood Cells ◽  
Coffee Consumption ◽  
Human Blood Cells

scholarly journals Corrigendum to “Dark coffee consumption protects human blood cells from spontaneous DNA damage” [J. Funct. Foods 55 (2019) 285–295]

2020 ◽  
Vol 71 ◽  
pp. 103989
Author(s):  
Gudrun Pahlke ◽  
Eva Attakpah ◽  
Georg Aichinger ◽  
Katarina Ahlberg ◽  
Christina Maria Hochkogler ◽  
...  
Keyword(s):  
Dna Damage ◽  
Human Blood ◽  
Blood Cells ◽  
Coffee Consumption ◽  
Human Blood Cells

In vitro DNA damage by Casiopeina II-gly in human blood cells

2016 ◽  
Vol 40 (2) ◽  
pp. 164-170 ◽  
Author(s):  
Juan José Rodríguez-Mercado ◽  
Diana Florín-Ramírez ◽  
Lucila Álvarez-Barrera ◽  
Mario Agustín Altamirano-Lozano
Keyword(s):  
Dna Damage ◽  
Human Blood ◽  
Blood Cells ◽  
Human Blood Cells

Evaluation of cytogenetic and DNA damage caused by thallium(I) acetate in human blood cells

2013 ◽  
Vol 30 (5) ◽  
pp. 572-580 ◽  
Author(s):  
Juan J. Rodríguez-Mercado ◽  
Heriberto Hernández-de la Cruz ◽  
Miriam Felipe-Reyes ◽  
Eduardo Jaramillo-Cruz ◽  
Mario A. Altamirano-Lozano
Keyword(s):  
Dna Damage ◽  
Human Blood ◽  
Blood Cells ◽  
Human Blood Cells

Genotoxicity of radiofrequency signals. I. Investigation of DNA damage and micronuclei induction in cultured human blood cells

2002 ◽  
Vol 23 (2) ◽  
pp. 113-126 ◽  
Author(s):  
Raymond R. Tice ◽  
Graham G. Hook ◽  
Maria Donner ◽  
Donald I. McRee ◽  
Arthur W. Guy
Keyword(s):  
Dna Damage ◽  
Human Blood ◽  
Blood Cells ◽  
Human Blood Cells

scholarly journals Usnic Acid and Usnea barbata (L.) F.H. Wigg. Dry Extracts Promote Apoptosis and DNA Damage in Human Blood Cells through Enhancing ROS Levels

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1171
Author(s):  
Violeta Popovici ◽  
Elena Matei ◽  
Georgeta Camelia Cozaru ◽  
Mariana Aschie ◽  
Laura Bucur ◽  
...  
Keyword(s):  
Dna Damage ◽  
Human Blood ◽  
Blood Cells ◽  
Cytotoxic Effect ◽  
Usnic Acid ◽  
Human Blood Cells ◽  
Late Apoptosis ◽  
High Concentrations ◽  
Induced Apoptosis ◽  
Nuclear Apoptosis

Nowadays, numerous biomedical studies performed on natural compounds and plant extracts aim to obtain highly selective pharmacological activities without unwanted toxic effects. In the big world of medicinal plants, Usnea barbata (L) F.H. Wigg (U. barbata) and usnic acid (UA) are well-known for their therapeutical properties. One of the most studied properties is their cytotoxicity on various tumor cells. This work aims to evaluate their cytotoxic potential on normal blood cells. Three dry U. barbata extracts in various solvents: ethyl acetate (UBEA), acetone (UBA), and ethanol (UBE) were prepared. From UBEA we isolated usnic acid with high purity by semipreparative chromatography. Then, UA, UBA, and UBE dissolved in 1% dimethyl sulfoxide (DMSO) and diluted in four concentrations were tested for their toxicity on human blood cells. The blood samples were collected from a healthy non-smoker donor; the obtained blood cell cultures were treated with the tested samples. After 24 h, the cytotoxic effect was analyzed through the mechanisms that can cause cell death: early and late apoptosis, caspase 3/7 activity, nuclear apoptosis, autophagy, reactive oxygen species (ROS) level and DNA damage. Generally, the cytotoxic effect was directly proportional to the increase of concentrations, usnic acid inducing the most significant response. At high concentrations, usnic acid and U. barbata extracts induced apoptosis and DNA damage in human blood cells, increasing ROS levels. Our study reveals the importance of prior natural products toxicity evaluation on normal cells to anticipate their limits and benefits as potential anticancer drugs.

Sign in / Sign up

Export Citation Format

Share Document